The Novel Murine Ca2+-binding Protein, Scarf, Is Differentially Expressed during Epidermal Differentiation

Estrus is an important factor for the fecundity of sows, and it is involved in ovulation and hormone secretion in ovaries. To better understand the molecular mechanisms of porcine estrus, the expression patterns of ovarian mRNA at proestrus and estrus stages were analyzed using RNA sequencing technology. A total of 2,167 differentially expressed genes (DEGs) were identified (P≤0.05, log2 Ratio≥1), of which 784 were upregulated and 1,383 were downregulated in the estrus compared with the proestrus group. Gene Ontology (GO) enrichment indicated that these DEGs were mainly involved in the cellular process, single-organism process, cell and cell part, and binding and metabolic process. In addition, a pathway analysis showed that these DEGs were significantly enriched in 33 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including cell adhesion molecules, ECM-receptor interaction, and cytokine-cytokine receptor interaction. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) confirmed the differential expression of 10 selected DEGs. Many of the novel candidate genes identified in this study will be valuable for understanding the molecular mechanisms of the sow estrous cycle.

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The gene for the Alzheimer-associated beta-amyloid-binding protein (ERAB) is differentially expressed in the testicular Leydig cells of the azoospermic by w/wv mouse

European Journal of Biochemistry ◽

10.1046/j.1432-1327.1998.2580053.x ◽

1998 ◽

Vol 258 (1) ◽

pp. 53-60 ◽

Cited By ~ 16

Author(s):

Christoph Hansis ◽

Detlev Jahner ◽

Andrej-Nikolai Spiess ◽

Kay Boettcher ◽

Richard Ivell

Keyword(s):

Leydig Cells ◽

Binding Protein ◽

Beta Amyloid ◽

Differentially Expressed

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The novel murine calmodulin-binding protein Sha1 disrupts mitotic spindle and replication checkpoint functions in fission yeast

Journal of Cell Science ◽

10.1242/jcs.111.24.3609 ◽

1998 ◽

Vol 111 (24) ◽

pp. 3609-3619

Author(s):

R. Craig ◽

C. Norbury

Keyword(s):

Fission Yeast ◽

Mitotic Spindle ◽

Binding Protein ◽

Protein S ◽

Spindle Pole ◽

The Novel ◽

Checkpoint Control ◽

Binding Motifs ◽

Replication Checkpoint ◽

Calmodulin Binding

Entry into mitosis is normally blocked in eukaryotic cells that have not completed replicative DNA synthesis; this ‘S-M’ checkpoint control is fundamental to the maintenance of genomic integrity. Mutants of the fission yeast Schizosaccharomyces pombe defective in the S-M checkpoint fail to arrest the cell cycle when DNA replication is inhibited and hence attempt mitosis and cell division with unreplicated chromosomes, resulting in the ‘cut’ phenotype. In an attempt to identify conserved molecules involved in the S-M checkpoint we have screened a regulatable murine cDNA library in S. pombe and have identified cDNAs that induce the cut phenotype in cells arrested in S phase by hydroxyurea. One such cDNA encodes a novel protein with multiple calmodulin-binding motifs that, in addition to its effects on the S-M checkpoint, perturbed mitotic spindle functions, although spindle pole duplication was apparently normal. Both aspects of the phenotype induced by this cDNA product, which we term Sha1 (for spindle and hydroxyurea checkpoint abnormal), were suppressed by simultaneous overexpression of calmodulin. Sha1 is structurally related to the product of the Drosophila gene abnormal spindle (asp). These data suggest that calmodulin-binding protein(s) are important in the co-ordination of mitotic spindle functions with mitotic entry in fission yeast, and probably also in multicellular eukaryotes.

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Plasma proteome profiling reveals differentially expressed lipopolysaccharide-binding protein among leptospirosis patients

Journal of Microbiology Immunology and Infection ◽

10.1016/j.jmii.2018.12.015 ◽

2020 ◽

Vol 53 (1) ◽

pp. 157-162 ◽

Cited By ~ 3

Author(s):

Cheng-Yee Fish-Low ◽

Leslie Thian Lung Than ◽

King-Hwa Ling ◽

Qingsong Lin ◽

Zamberi Sekawi

Keyword(s):

Binding Protein ◽

Differentially Expressed ◽

Plasma Proteome ◽

Lipopolysaccharide Binding Protein ◽

Proteome Profiling ◽

Lipopolysaccharide Binding

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Identifying the functional part of heparin-binding protein (HBP) as a monocyte stimulator and the novel role of monocytes as HBP producers

Innate Immunity ◽

10.1177/1753425909354763 ◽

2010 ◽

Vol 17 (1) ◽

pp. 60-69 ◽

Cited By ~ 3

Author(s):

Morten Schou ◽

René Djurup ◽

Kjeld Norris ◽

Hans Flodgaard

Keyword(s):

Binding Protein ◽

The Novel ◽

Heparin Binding ◽

Heparin Binding Protein ◽

Functional Part

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Potential genes and pathways along with immune cells infiltration in the progression of atherosclerosis identified via microarray gene expression dataset re-analysis

Vascular ◽

10.1177/1708538120922700 ◽

2020 ◽

Vol 28 (5) ◽

pp. 643-654 ◽

Cited By ~ 1

Author(s):

Jing Xu ◽

Yuejin Yang

Keyword(s):

Differentially Expressed Genes ◽

Immune Cells ◽

Interaction Network ◽

Wilcoxon Test ◽

Differentially Expressed ◽

P Value ◽

The Novel ◽

Microarray Gene Expression ◽

Progression Of Atherosclerosis ◽

Geo Database

Objective Atherosclerosis is a chronic inflammatory process characterized by the accumulation and formation of lipid-rich plaques within the layers of the arterial wall. Although numerous studies have reported the underlying pathogenesis, no data-based studies have been conducted to analyze the potential genes and immune cells infiltration in the different stages of atherosclerosis via bioinformatics analysis. Methods In this study, we downloaded GSE100927 and GSE28829 from NCBI-GEO database. Gene ontology and pathway enrichment were performed via the DAVID database. The protein interaction network was constructed via STRING. Enriched hub genes were analyzed by the Cytoscape software. The evaluation of the infiltrating immune cells in the dataset samples was performed by the CIBERSORT algorithm. Results We identified 114 common upregulated differentially expressed genes and 22 common downregulated differentially expressed genes. (adjust p value < 0.01 and log FC ≥ 1). A cluster of 10 genes including CYBA, SLC11A1, FCER1G, ITGAM, ITGB2, CD53, ITGAX, VAMP8, CLEC5A, and CD300A were found to be significant. Through the deconvolution algorithm CIBERSORT, we analyzed the significant alteration of immune cells infiltration in the progression of atherosclerosis with the threshold of the Wilcoxon test at p value <0.05. Conclusions These results may reveal the underlying correlations between genes and immune cells in atherosclerosis, which enable us to investigate the novel insights for the development of treatments and drugs.

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