Catalase Takes Part in Rat Liver Mitochondria Oxidative Stress Defense

: In recent years, the applications of nanoparticles have received a great attention due to their industrial and biomedical applications, while their beneficial effects suffer from controversial results at clinical stages. In the current study, cytotoxicity of cerium oxide (CeNP) nanoparticles (100 nm) were evaluated using mitochondria derived from wistar rat's liver. Isolated mitochondria from rat’s liver were divided into 7 groups including group 1 as control and group 2 to 7 as treatment group with different doses of CeNP (5, 10, 50, 100, 250 and 500mg/ml, respectively), for 24,48 and 72 hours. After exposure, oxidative stress biomarkers such as total ‎antioxidant capacity (TAC), lipid peroxidation (LPO), total thiol groups (TTG), catalase activity (CAT) and mitochondrial viability, were determined in isolated rat liver mitochondria. Results have shown that CeNPs increase TAC, TTG, CAT, LPO and viability of mitochondria in various exposure times and confirm antioxidant properties of CeNPs in mithocondria while mitochondria is a main source for the generation of reactive oxygen species (ROS).

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Alleviation of enhanced oxidative stress and oxygen consumption of l-thyroxine induced hyperthyroid rat liver mitochondria by vitamin E and curcumin

Chemico-Biological Interactions ◽

10.1016/j.cbi.2008.02.005 ◽

2008 ◽

Vol 173 (2) ◽

pp. 105-114 ◽

Cited By ~ 52

Author(s):

U. Subudhi ◽

K. Das ◽

B. Paital ◽

S. Bhanja ◽

G.B.N. Chainy

Keyword(s):

Oxidative Stress ◽

Oxygen Consumption ◽

Vitamin E ◽

Rat Liver ◽

Liver Mitochondria ◽

Rat Liver Mitochondria

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Tetrandrine concentrations not affecting oxidative phosphorylation protect rat liver mitochondria from oxidative stress

Mitochondrion ◽

10.1016/j.mito.2006.06.002 ◽

2006 ◽

Vol 6 (4) ◽

pp. 176-185 ◽

Cited By ~ 46

Author(s):

Maria A.S. Fernandes ◽

José B.A. Custódio ◽

Maria S. Santos ◽

António J.M. Moreno ◽

Joaquim A.F. Vicente

Keyword(s):

Oxidative Stress ◽

Oxidative Phosphorylation ◽

Rat Liver ◽

Liver Mitochondria ◽

Rat Liver Mitochondria

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Toxicity of thallium on isolated rat liver mitochondria: The role of oxidative stress and MPT pore opening

Environmental Toxicology ◽

10.1002/tox.21900 ◽

2013 ◽

Vol 30 (2) ◽

pp. 232-241 ◽

Cited By ~ 22

Author(s):

M R Eskandari ◽

Vida Mashayekhi ◽

Majid Aslani ◽

Mir-Jamal Hosseini

Keyword(s):

Oxidative Stress ◽

Rat Liver ◽

Liver Mitochondria ◽

Rat Liver Mitochondria ◽

Isolated Rat Liver ◽

Pore Opening ◽

Isolated Rat

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Toxicity of Evodiae fructus on Rat Liver Mitochondria: The Role of Oxidative Stress and Mitochondrial Permeability Transition

Molecules ◽

10.3390/molecules191221168 ◽

2014 ◽

Vol 19 (12) ◽

pp. 21168-21182 ◽

Cited By ~ 21

Author(s):

Qingyan Cai ◽

Jingjing Wei ◽

Wei Zhao ◽

Si Shi ◽

Yu Zhang ◽

...

Keyword(s):

Oxidative Stress ◽

Rat Liver ◽

Mitochondrial Permeability Transition ◽

Permeability Transition ◽

Liver Mitochondria ◽

Rat Liver Mitochondria ◽

Mitochondrial Permeability

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Oxaliplatin-induced Oxidative Stress Provokes Toxicity in Isolated Rat Liver Mitochondria

Archives of Medical Research ◽

10.1016/j.arcmed.2015.10.002 ◽

2015 ◽

Vol 46 (8) ◽

pp. 597-603 ◽

Cited By ~ 12

Author(s):

Heena Tabassum ◽

Mohammad Waseem ◽

Suhel Parvez ◽

M. Irfan Qureshi

Keyword(s):

Oxidative Stress ◽

Rat Liver ◽

Liver Mitochondria ◽

Rat Liver Mitochondria ◽

Isolated Rat Liver ◽

Isolated Rat

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BISPHENOL A DOSE- AND TIME-DEPENDENTLY INDUCES OXIDATIVE STRESS IN RAT LIVER MITOCHONDRIA EX VIVO

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i9.26750 ◽

2018 ◽

Vol 11 (9) ◽

pp. 98 ◽

Cited By ~ 2

Author(s):

Mousumi Dutta ◽

Goutam Paul

Keyword(s):

Oxidative Stress ◽

Bisphenol A ◽

Rat Liver ◽

Ex Vivo ◽

Liver Mitochondria ◽

Time Dependent ◽

Incubation Mixture ◽

Rat Liver Mitochondria ◽

Control Group ◽

Dependent Manner

Objective: The probable toxic effects of bisphenol A (BPA) on different physiological functions have been reported in animal models. The role of BPA in mitochondrial oxidative stress has not been reported till date. The present study is aimed to elucidate dose- and time-dependent oxidative stress generation by BPA, respectively, in rat liver mitochondria in ex vivo model. Methods: The incubation mixture of BPA-treated groups containing mitochondria, 50 mM potassium phosphate buffer (pH 7.4), and different concentrations of BPA (20–160 μM/ml) (dissolved in 12% DMSO) in a final volume of 1.0 ml was incubated at 37°C in incubator for different time durations (30 min–2 h). Whereas, the incubation mixture of control group contained DMSO (12%), mitochondria and 50 mM potassium phosphatebuffer (pH 7.4).’ will be replaced by ‘Whereas, the incubation mixture of control group contained the same constituents except BPA. Result: We have observed significant decrease in mitochondrial intactness incubated with BPA in dose- and time-dependent manner under bright field and confocal microscopic study compared to control. Further, we have observed a decrease in mitochondrial reduced glutathione (GSH) content and increase in lipid peroxidation and protein carbonylation levels in dose- and time-dependent manner in BPA-exposed mitochondria. We have found a significant increase in the activity of Mn-superoxide dismutase and decrease in the activities of GSH peroxidase, GSH reductase, pyruvate dehydrogenase, and other three enzymes of Kreb’s cycle dose and time dependently in BPA-exposed mitochondria. The results indicate that exposure to BPA leads to decrease in intactness of mitochondria and increase in oxidative stress in mitochondria isolated from rat liver in a dose- and time-dependent manner. Conclusion: It can be concluded that the incubation of mitochondria isolated from rat liver with BPA, caused oxidative stress-mediated damages in mitochondria in both dose- and time-dependent manners.

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