Validation of a simple avidin-biotin detection method for Helix pomatia lectin (HPA) binding as a prognostic marker in cancer

2003 ◽  
Vol 105 (3) ◽  
pp. 205-212 ◽  
Author(s):  
Susan A. Brooks ◽  
Diane Wilkinson
Keyword(s):  
Prognostic Marker ◽  
Detection Method ◽  
Helix Pomatia ◽  
Helix Pomatia Lectin

Lectin-gold labeling of glycoconjugates in normal and Brattleboro rat papilla: effect of vasopressin

1988 ◽  
Vol 254 (3) ◽  
pp. C450-C458 ◽  
Author(s):  
P. Weyer ◽  
D. Brown ◽  
L. Orci
Keyword(s):  
Epithelial Cell ◽  
Plasma Membranes ◽  
Collecting Duct ◽  
Helix Pomatia ◽  
Major Effect ◽  
Urinary Concentration ◽  
Brattleboro Rats ◽  
Cell Plasma ◽  
Collecting Ducts ◽  
Helix Pomatia Lectin

Some reports suggest that the plasma membrane glycocalyx of collecting duct epithelial cells, as well as interstitial glycoconjugates, may be involved in vasopressin action and urinary concentration. In view of this, we have used the lectin-gold technique to map and quantify Helix pomatia lectin (HPL)-binding sites in the inner medulla of kidneys from normal Long-Evans rats, vasopressin-deficient Brattleboro rats, and Brattleboro rats treated for up to 5 wk with exogenous vasopressin. The results show that the labeling of epithelial cell plasma membranes from collecting ducts and thin limbs of Henle is not different between normal and Brattleboro rats, and the labeling is not modified by chronic vasopressin treatment. In contrast, the heavy interstitial labeling seen in normal rats is virtually absent from Brattleboro rats, but it is progressively restored by chronic vasopressin treatment of Brattleboro rats. These results show that vasopressin does not modify HPL-binding glycoconjugates on epithelial cell plasma membranes, but that vasopressin treatment has a major effect on HPL-binding glycoconjugates in the medullary interstitium.

scholarly journals Identification of group C Streptococcus with Helix pomatia lectin in use for blood grouping.

1994 ◽  
Vol 49 (5/6) ◽  
pp. 779-785
Author(s):  
Tatsuo NAGAI ◽  
Yuko YANAGISAWA ◽  
Werner Köhler ◽  
Otto Prokop
Keyword(s):  
Helix Pomatia ◽  
Helix Pomatia Lectin ◽  
Blood Grouping

scholarly journals Histochemistry to detect Helix pomatia lectin binding in breast cancer: methodology makes a difference.

1996 ◽  
Vol 44 (5) ◽  
pp. 519-524 ◽  
Author(s):  
S A Brooks ◽  
M Lymboura ◽  
U Schumacher ◽  
A J Leathem
Keyword(s):  
Breast Cancer ◽  
Indirect Method ◽  
Direct Method ◽  
Lectin Binding ◽  
Helix Pomatia ◽  
Biological Behavior ◽  
Breast Cancers ◽  
Term Survival ◽  
Direct Technique ◽  
Helix Pomatia Lectin

A number of studies have shown that altered cellular glycosylation, as detected by binding of Helix pomatia lectin to paraffin sections, is associated with metastatic disease and consequent poor patient prognosis in breast and other cancers. In a 24-year retrospective study, sections of 373 primary breast cancers were stained for binding of the lectin using two different histochemical techniques: a direct method (using peroxidase-conjugated lectin) and an indirect method (using native, unconjugated lectin). Similar percentages of cases were positive (79%) and negative (21%) for lectin binding with either technique, but there was enormous inconsistency when individual cases were examined. A total of 38/373 (10.2%) cases that were negative by the indirect method were positive by the direct method, and 37/373 (9.9%) cases that were negative by the direct method were positive by the indirect method. Life tables calculated for lectin staining vs nonstaining cases showed a very strong correlation between lectin binding and long-term survival (p < 0.0001) when staining was performed by the indirect method, but only very weak correlation with prognosis (p < 0.03, borderline significance) when the direct technique was employed. SDS-PAGE revealed that there were differences in breast cancer glycoproteins recognized by native lectin and peroxidase-conjugated lectin immobilized on Sepharose 4B affinity beads. Helix pomatia lectin binding appears to be an intriguing and potentially valuable marker of biological behavior in breast cancer. This study emphasizes the importance of selecting an appropriate immunohistochemical technique for its visualization.

HELIX POMATIA LECTIN-BINDING AND PREDICTIVE VALUE IN BREAST-CANCER

The Lancet ◽  
1987 ◽  
Vol 330 (8568) ◽  
pp. 1145
Author(s):  
F.J. Grundbacher ◽  
A. Leathem ◽  
Susan Brooks
Keyword(s):  
Breast Cancer ◽  
Predictive Value ◽  
Lectin Binding ◽  
Helix Pomatia ◽  
Helix Pomatia Lectin
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