Effect of plasma membrane-associated proteins on acquisition of freezing tolerance in Arabidopsis thaliana.

Abstract Aquaporins play a major role in plant water uptake at both optimal and environmentally stressed conditions. However, the functional specificity of aquaporins under cold remains obscure. To get a better insight to the role of aquaporins in cold acclimation and freezing tolerance, we took an integrated approach of physiology, transcript profiling and cell biology in Arabidopsis thaliana. Cold acclimation resulted in specific upregulation of PIP1;4 and PIP2;5 aquaporin (plasma membrane intrinsic proteins) expression, and immunoblotting analysis confirmed the increase in amount of PIP2;5 protein and total amount of PIPs during cold acclimation, suggesting that PIP2;5 plays a major role in tackling the cold milieu. Although single mutants of pip1;4 and pip2;5 or their double mutant showed no phenotypic changes in freezing tolerance, they were more sensitive in root elongation and cell survival response under freezing stress conditions compared with the wild type. Consistently, a single mutation in either PIP1;4 or PIP2;5 altered the expression of a number of aquaporins both at the transcriptional and translational levels. Collectively, our results suggest that aquaporin members including PIP1;4 and PIP2;5 function in concert to regulate cold acclimation and freezing tolerance responses.

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Identification of MAMP-Responsive Plasma Membrane-Associated Proteins in Arabidopsis thaliana Following Challenge with Different LPS Chemotypes from Xanthomonas campestris

Pathogens ◽

10.3390/pathogens9100787 ◽

2020 ◽

Vol 9 (10) ◽

pp. 787

Author(s):

Raeesa H. Hussan ◽

Ian A. Dubery ◽

Lizelle A. Piater

Keyword(s):

Arabidopsis Thaliana ◽

Plasma Membrane ◽

Xanthomonas Campestris ◽

Induced Defense ◽

Defense Responses ◽

Structural Features ◽

Wild Type ◽

Core Oligosaccharide ◽

Associated Proteins ◽

Induced Defense Responses

Lipopolysaccharides (LPS) are recognized as microbe-associated molecular patterns (MAMPs) responsible for eliciting defense-related responses and while the effects have been well-documented in mammals, there is a lack of knowledge regarding the mechanism of perception in plant systems and recognized structural moieties within the macromolecular lipoglycan structure. Thus, identification of the LPS plasma membrane (PM) receptor(s)/receptor complex in Arabidopsis thaliana through proteomics will contribute to a deeper understanding of induced defense responses. As such, structurally characterized LPS chemotypes from Xanthomonas campestris pv. campestris (Xcc) wild-type 8004 (prototypical smooth-type LPS) and mutant 8530 (truncated core with no O–chain) strains were utilized to pre-treat A. thaliana plants. The associated proteomic response/changes within the PM were compared over a 24 h period using mass spectrometry-based methodologies following three variants of LPS-immobilized affinity chromatography. This resulted in the identification of proteins from several functional categories, but importantly, those involved in perception and defense. The distinct structural features between wild-type and mutant LPS are likely responsible for the differential changes to the proteome profiles, and many of the significant proteins were identified in response to the wild-type Xcc LPS where it is suggested that the core oligosaccharide and O-chain participate in recognition by receptor-like kinases (RLKs) in a multiprotein complex and, notably, varied from that of the mutant chemotype.

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Faculty Opinions recommendation of The plasma membrane-bound phospholipase Ddelta enhances freezing tolerance in Arabidopsis thaliana.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1018179.206660 ◽

2004 ◽

Author(s):

Ramón Serrano

Keyword(s):

Arabidopsis Thaliana ◽

Plasma Membrane ◽

Freezing Tolerance ◽

Membrane Bound

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Faculty Opinions recommendation of Overexpression of a plasma membrane Na+/H+ antiporter gene improves salt tolerance in Arabidopsis thaliana.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1011530.181017 ◽

2003 ◽

Author(s):

Jeff Woessner

Keyword(s):

Arabidopsis Thaliana ◽

Plasma Membrane ◽

Salt Tolerance

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Faculty Opinions recommendation of Cold-inducible zinc finger-containing glycine-rich RNA-binding protein contributes to the enhancement of freezing tolerance in Arabidopsis thaliana.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1026431.323264 ◽

2005 ◽

Author(s):

Michael Thomashow

Keyword(s):

Arabidopsis Thaliana ◽

Zinc Finger ◽

Freezing Tolerance ◽

Rna Binding ◽

Binding Protein ◽

Rna Binding Protein

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Faculty Opinions recommendation of A fast brassinolide-regulated response pathway in the plasma membrane of Arabidopsis thaliana.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.8044970.13563054 ◽

2011 ◽

Author(s):

Christian Hardtke

Keyword(s):

Arabidopsis Thaliana ◽

Plasma Membrane

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Biotinylation and Purification of Plasma Membrane-associated Proteins from Rodent Cultured Neurons

BIO-PROTOCOL ◽

10.21769/bioprotoc.1807 ◽

2016 ◽

Vol 6 (10) ◽

Author(s):

Margarida Caldeira ◽

Joana Ferreira ◽

Ana Carvalho ◽

Carlos Duarte

Keyword(s):

Plasma Membrane ◽

Cultured Neurons ◽

Associated Proteins

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A family of pathogen-induced cysteine-rich transmembrane proteins is involved in plant disease resistance

Planta ◽

10.1007/s00425-021-03606-3 ◽

2021 ◽

Vol 253 (5) ◽

Author(s):

Marciel Pereira Mendes ◽

Richard Hickman ◽

Marcel C. Van Verk ◽

Nicole M. Nieuwendijk ◽

Anja Reinstädler ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Plasma Membrane ◽

Disease Resistance ◽

Plant Disease Resistance ◽

Transmembrane Proteins ◽

Series Data ◽

Immune Gene ◽

Biological Processes ◽

Hypocotyl Growth ◽

Enhanced Resistance

Abstract Main conclusion Overexpression of pathogen-induced cysteine-rich transmembrane proteins (PCMs) in Arabidopsis thaliana enhances resistance against biotrophic pathogens and stimulates hypocotyl growth, suggesting a potential role for PCMs in connecting both biological processes. Abstract Plants possess a sophisticated immune system to protect themselves against pathogen attack. The defense hormone salicylic acid (SA) is an important player in the plant immune gene regulatory network. Using RNA-seq time series data of Arabidopsis thaliana leaves treated with SA, we identified a largely uncharacterized SA-responsive gene family of eight members that are all activated in response to various pathogens or their immune elicitors and encode small proteins with cysteine-rich transmembrane domains. Based on their nucleotide similarity and chromosomal position, the designated Pathogen-induced Cysteine-rich transMembrane protein (PCM) genes were subdivided into three subgroups consisting of PCM1-3 (subgroup I), PCM4-6 (subgroup II), and PCM7-8 (subgroup III). Of the PCM genes, only PCM4 (also known as PCC1) has previously been implicated in plant immunity. Transient expression assays in Nicotiana benthamiana indicated that most PCM proteins localize to the plasma membrane. Ectopic overexpression of the PCMs in Arabidopsis thaliana resulted in all eight cases in enhanced resistance against the biotrophic oomycete pathogen Hyaloperonospora arabidopsidis Noco2. Additionally, overexpression of PCM subgroup I genes conferred enhanced resistance to the hemi-biotrophic bacterial pathogen Pseudomonas syringae pv. tomato DC3000. The PCM-overexpression lines were found to be also affected in the expression of genes related to light signaling and development, and accordingly, PCM-overexpressing seedlings displayed elongated hypocotyl growth. These results point to a function of PCMs in both disease resistance and photomorphogenesis, connecting both biological processes, possibly via effects on membrane structure or activity of interacting proteins at the plasma membrane.

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