Porcine Kidney Tissue Based Membrane Electrode for Glucosamine-6-Phosphate

1985 ◽  
Vol 18 (13) ◽  
pp. 1635-1646 ◽  
Author(s):  
Yi Long Ma ◽  
G. A. Rechnitz
Keyword(s):  
Kidney Tissue ◽  
Membrane Electrode ◽  
Porcine Kidney

The Course of Enzymatically Induced Lipid Peroxidation in Homogenized Porcine Kidney Tissue

1998 ◽  
Vol 53 (5-6) ◽  
pp. 431-438 ◽  
Author(s):  
Uwe Kießling ◽  
Gerhard Spiteller
Keyword(s):  
Lipid Peroxidation ◽  
Octadecadienoic Acid ◽  
Tissue Injury ◽  
Kidney Tissue ◽  
Oxidation Products ◽  
Mammalian Tissue ◽  
Porcine Kidney ◽  
Octadecanoic Acid ◽  
Biological Environment ◽  
Primary Oxidation

Homogenization of mammalian tissue - exemplified by porcine kidney - causes enzymatically induced lipid peroxidation (LPO) processes proven by measuring the amounts of the typical lipid peroxidation products 9- and 13-hydroxy-octadecadienoic acid (HODE) either after homogenization in aqueous (activation of enzymes) or an organic (inactivated enzymes) solvent. A kinetic study revealed that the level of the 9- and 13-isomer reached maximum values 6 hours after tissue injury. Within one day the amount of these primary oxidation products was reduced fast, indicating that they undergo degradation in their biological environment. In contrast, the level of 10-hydroxy-octadecanoic acid - obviously derived from LPO of oleic acid - increased continuously even after one day. These observations reflect that the generation and degradation of hydroperoxides occurs at different rates which might be of interest in pathological processes connected with tissue injury, e.g. myocardial infarction.

scholarly journals Integrative Omics Reveals Subtle Molecular Perturbations Following Ischemic Conditioning in a Porcine Kidney Transplant Model

2021 ◽  
Author(s):  
Darragh P. O’Brien ◽  
Adam M. Thorne ◽  
Honglei Huang ◽  
Xuan Yao ◽  
Peter Søndergaard Thyrrestrup ◽  
...  
Keyword(s):  
Kidney Transplant ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Kidney Tissue ◽  
Kidney Graft ◽  
Porcine Kidney ◽  
Matrix Assembly ◽  
Ischemic Conditioning ◽  
Transplant Model ◽  
Integrative Omics

ABSTRACTBackgroundRemote Ischemic Conditioning (RIC) has been proposed as a therapeutic intervention to circumvent the Ischemia/reperfusion injury (IRI) that is inherent to organ transplantation. Using a porcine kidney transplant model, we aimed to decipher the subclinical molecular effects of a RIC regime, compared to non-RIC controls.MethodsKidney pairs (n = 8+8) were extracted from brain dead donor pigs and transplanted in juvenile recipient pigs following a period of cold ischemia. One of the two kidney recipients in each pair was subjected to RIC prior to kidney graft reperfusion, while the other served as non-RIC control. We designed an advanced integrative Omics strategy combining transcriptomics, proteomics, and phosphoproteomics to deduce molecular signatures in kidney tissue that could be attributed to RIC.ResultsIn kidney grafts taken out 10 h after transplantation we detected minimal molecular perturbations following RIC compared to non-RIC at the transcriptome level, but more pronounced effects at the proteome level. In particular, we noted that RIC resulted in suppression of tissue inflammatory profiles. Furthermore, an accumulation of muscle extracellular matrix assembly proteins in kidney tissues was detected at the protein level, which may be in response to muscle tissue damage and/or fibrosis.ConclusionsOur data identifies subtle molecular phenotypes in porcine kidneys following RIC and this knowledge could potentially aid optimisation of remote ischaemia protocols in renal transplantation.

Freeze-Etch Crystal-Like Structures in Membranous Glomerulosclerosis

1969 ◽  
Vol 27 ◽  
pp. 394-395
Author(s):  
Burton B. Silver
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Sodium Hypochlorite ◽  
Kidney Tissue ◽  
Extraction Process ◽  
Donor Kidney ◽  
Unknown Substance ◽  
Etched Surface ◽  
Standard Fixation ◽  
Diseased Kidney

Tissue from a non-functional kidney affected with chronic membranous glomerulosclerosis was removed at time of trnasplantation. Recipient kidney tissue and donor kidney tissue were simultaneously fixed for electron microscopy. Primary fixation was in phosphate buffered gluteraldehyde followed by infiltration in 20 and then 40% glycerol. The tissues were frozen in liquid Freon and finally in liquid nitrogen. Fracturing and replication of the etched surface was carried out in a Denton freeze-etch device. The etched surface was coated with platinum followed by carbon. These replicas were cleaned in a 50% solution of sodium hypochlorite and mounted on 400 mesh copper grids. They were examined in an Siemens Elmiskop IA. The pictures suggested that the diseased kidney had heavy deposits of an unknown substance which might account for its inoperative state at the time of surgery. Such deposits were not as apparent in light microscopy or in the standard fixation methods used for EM. This might have been due to some extraction process which removed such granular material in the dehydration steps.

A Comparison Between Sterological Point Counting and Digitizing Tablets

1985 ◽  
Vol 43 ◽  
pp. 666-667
Author(s):  
John M. Basgen ◽  
Eileen N. Ellis ◽  
S. Michael Mauer ◽  
Michael W. Steffes
Keyword(s):  
Electron Microscopy ◽  
Kidney Tissue ◽  
Volume Density ◽  
Diabetic Patients ◽  
Normal Kidney ◽  
Point Counting ◽  
Normal Kidney Tissue ◽  
Biopsy Specimens ◽  
Mitochondrial Volume ◽  
Kidney Lesions

To determine the efficiency of methods of quantitation of the volume density of components within kidney biopsies, techniques involving a semi-automatic digitizing tablet and stereological point counting were compared.Volume density (Vv) is a parameter reflecting the volume of a component to the volume that contains the component, e.g., the fraction of cell volume that is made up of mitochondrial volume. The units of Vv are μm3 /μm3.Kidney biopsies from 15 patients were used. Five were donor biopsies performed at the time of kidney transplantation (patients 1-5, TABLE 1) and were considered normal kidney tissue. The remaining biopsies were obtained from diabetic patients with a spectrum of diabetic kidney lesions. The biopsy specimens were fixed and embedded according to routine electron microscogy protocols. Three glomeruli from each patient were selected randomly for electron microscopy. An average of 12 unbiased and systematic micrographs were obtained from each glomerulus and printed at a final magnification of x18,000.

Electron microscopic study of the membrane glycoproteins in the rat kidney after cisplatin treatment

1989 ◽  
Vol 47 ◽  
pp. 912-913
Author(s):  
S.K. Aggarwal
Keyword(s):  
Alkaline Phosphatase ◽  
Rat Kidney ◽  
Light And Electron Microscopy ◽  
Kidney Tissue ◽  
Membrane Glycoproteins ◽  
Electron Microscopic ◽  
Before And After ◽  
Interstrand Cross Links ◽  
Cross Links

The proposed primary mechanism of action of the anticancer drug cisplatin (Cis-DDP) is through its interaction with DNA, mostly through DNA intrastrand cross-links or DNA interstrand cross-links. DNA repair mechanisms can circumvent this arrest thus permitting replication and transcription to proceed. Various membrane transport enzymes have also been demonstrated to be effected by cisplatin. Glycoprotein alkaline phosphatase was looked at in the proximal tubule cells before and after cisplatin both in vivo and in vitro for its inactivation or its removal from the membrane using light and electron microscopy.Outbred male Swiss Webster (Crl: (WI) BR) rats weighing 150-250g were given ip injections of cisplatin (7mg/kg). Animals were killed on day 3 and day 5. Thick slices (20-50.um) of kidney tissue from treated and untreated animals were fixed in 1% buffered glutaraldehyde and 1% formaldehyde (0.05 M cacodylate buffer, pH 7.3) for 30 min at 4°C. Alkaline phosphatase activity and carbohydrates were demonstrated according to methods described earlier.

Localization of cell-surface and basement-membrane heparan-sulfate proteoglycans using the malaria circumsporozoite protein

1994 ◽  
Vol 52 ◽  
pp. 294-295
Author(s):  
U. Frevert ◽  
S. Sinnis ◽  
C. Cerami ◽  
V. Nussenzweig
Keyword(s):  
Heparan Sulfate ◽  
Protein A ◽  
Kidney Tissue ◽  
Plasmodium Species ◽  
Its Region ◽  
Basement Membranes ◽  
Heparan Sulfate Proteoglycans ◽  
Infected Mosquito ◽  
Complement Components ◽  
Region Ii

Malaria sporozoites, which invade hepatocytes within minutes after transmission by an infected mosquito, are covered with the circumsporozoite (CS) protein, which in all Plasmodium species contains the conserved region II-plus. This region is also found as a cell-adhesive motif in a variety of host proteins like thrombospondin, properdin and the terminal complement components.The CS protein with its region II-plus specifically binds to heparan sulfate proteoglycans (HSPG) on the basolateral surface of hepatocytes in the space of Disse (FIG. 1), to certain basolateral cell membranes and basement membranes of the kidney (FIG. 2) as well as to heparin in the granules of connective tissue mast cells. The distribution of the HSPG receptors for the CS protein was examined by incubation of Lowicryl K4M or LR White sections of liver and kidney tissue with the recombinant CS ligand, whose binding sites were detected with a monoclonal anti-CS antibody and protein A gold.

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