Genotoxic potential of aqueous extracts ofArtemisia verlotorumon the cell cycle ofAllium cepa

Introduction: Bioflavonoids delphinidin (2-(3,4,5-Trihydroxyphenyl)chromenylium-3,5,7-triol) and luteolin (2-(3,4-Dihydroxyphenyl)-5,7-dihydroxy-4-chromenone) have been recognized as promising antioxidants and anticancer substances. Due to their extensive use, the goal of the research was to determine whether they have any genotoxic potential in vitro.Methods: Analysis of genotoxic potential was performed applying chromosome aberrations test in human lymphocyte culture, as this kind of research was not conducted abundantly for these two bioflavonoids. Delphinidin and luteolin were dissolved in DMSO and added to cultures in final concentrations of 25, 50 and 100 μM.Results: In human lymphocytes cultures Delphinidin induced PCDs in all treatments, potentially affecting the cell cycle and topoisomerase II activity. In concentration of 50 μM luteolin showed strong genotoxic effects and caused significant reduction of cell proliferation.Conclusion: Luteolin exhibited certain genotoxic and cytostatic potential. Delphinidin was not considered genotoxic, however its impact on mitosis, especially topoisomerase II activity, was revealed.

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Non-aqueous extracts of Curcuma mangga rhizomes induced cell death in human colorectal adenocarcinoma cell line (HT29) via induction of apoptosis and cell cycle arrest at G0/G1 phase

Asian Pacific Journal of Tropical Medicine ◽

10.1016/j.apjtm.2015.12.003 ◽

2016 ◽

Vol 9 (1) ◽

pp. 8-18 ◽

Cited By ~ 7

Author(s):

Gin Wah Hong ◽

Sok Lai Hong ◽

Guan Serm Lee ◽

Hashim Yaacob ◽

Sri Nurestri Abd Malek

Keyword(s):

Cell Cycle ◽

Cell Death ◽

Cell Line ◽

Colorectal Adenocarcinoma ◽

G1 Phase ◽

Aqueous Extracts ◽

Adenocarcinoma Cell Line ◽

Adenocarcinoma Cell ◽

Cycle Arrest ◽

Induction Of Apoptosis

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Antiproliferative action of aqueous extracts of Hymenaea stigonocarpa Mart. (Fabaceae) on the cell cycle of Allium cepa L.

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201420130163 ◽

2014 ◽

Vol 86 (3) ◽

pp. 1147-1150 ◽

Cited By ~ 10

Author(s):

LOURRAN P. LACERDA ◽

GEIZ MALAQUIAS ◽

ANA PAULA PERON

Keyword(s):

Cell Cycle ◽

Allium Cepa ◽

Test System ◽

Control Group ◽

Aqueous Extracts ◽

Root Cells ◽

Antiproliferative Action ◽

Allium Cepa L ◽

Meristematic Root ◽

Number Of Cells

In this study we evaluated the action of crude aqueous extracts obtained from rhytidome of Hymenaea stigonocarpa (jatobá-do-cerrado) on Allium cepa meristematic root cells in three concentrations: 0.082, 0.164, 0.328g/mL, at exposure times of 24 and 48 h. The slides were prepared by the crushing technique, and cells analyzed throughout the cell cycle, totaling 5000 for each control group and concentration. It was found that all three concentrations, including the lowest which is considered ideal for use, in all exposure times, had significant antiproliferative action on the cell cycle of this test system. For cells under division, we observed a high number of cells in prophase. Therefore, under the conditions studied H. stigonocarpa indicated to be cytotoxic.

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Anti-Proliferative and Genotoxic Activities of the Helichrysum petiolare Hilliard & B.L. Burtt

Scientia Pharmaceutica ◽

10.3390/scipharm88040049 ◽

2020 ◽

Vol 88 (4) ◽

pp. 49

Author(s):

Idowu Jonas Sagbo ◽

Wilfred Otang-Mbeng

Keyword(s):

Cell Cycle ◽

Chemical Constituents ◽

Scientific Evidence ◽

Micronucleus Assay ◽

Vero Cells ◽

Melanoma Cells ◽

Anticancer Properties ◽

Genotoxic Potential ◽

B16f10 Cells ◽

Cell Arrest

Helichrysum petiolare (Asteraceae family) is part of the Helichrysum genus which comprises of an estimated 600 species. Several parts of the plant have been used traditionally for the treatment of various ailments, such as cough, infection, asthma, chest problems, diabetes and wounds. Given its various chemical constituents with anticancer properties, there has been no scientific evidence of its usage for the treatment of cancer. This study aims to investigate the anti-proliferative and genotoxic activities of H. petiolare methanol extract. The cytotoxic effect and cell cycle analysis of mouse melanoma cells (B16F10) and human melanoma cells (MeWo) were assessed using the ImageXpress Micro XLS Widefield High-Content Analysis System. The genotoxic potential of the extract towards Vero cells was also assessed using the micronucleus assay. The extract displayed cytotoxicity towards B16F10 and MeWo skin melanoma cells, thereby showing a dose-dependent decrease in cell density. This was preceded by cell cycle arrest in B16F10 cells at the S phase and MeWo cell arrest at the early M phase with a significant increase in apoptosis in both cells. Furthermore, the extract displayed genotoxic potential at the tested concentrations (12.5–200 μg/mL). Overall, the results revealed that H. petiolare extract may have the potential to eradicate skin cancer.

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Cytotoxic and Genotoxic Effects of Macroptilium lathyroides

Planta Daninha ◽

10.1590/s0100-83582018360100043 ◽

2018 ◽

Vol 36 (0) ◽

Cited By ~ 3

Author(s):

M.S.A. SILVA ◽

O.M. YAMASHITA ◽

A.A.B. ROSSI ◽

G. CONCENÇO ◽

M.A.C. CARVALHO ◽

...

Keyword(s):

Cell Cycle ◽

Chromosomal Abnormalities ◽

Test Organism ◽

Optical Microscope ◽

Aqueous Extracts ◽

Genotoxic Effects ◽

Qualitative Level ◽

Leaves And Roots ◽

Macroptilium Lathyroides ◽

Number Of Cells

ABSTRACT: The monitoring of phytochemicals with potentially toxic properties can be assessed by observing the physiological and cellular alterations of the test organism exposed. This present study aimed to evaluate the cytotoxic and genotoxic potential of aqueous extracts of leaves and roots of Macroptilium lathyroides weed on the cell cycle of lettuce. Bioassays were conducted in a germinator (25 oC) with aqueous extract at concentrations of 0, 5, 10, 20 and 40% p v-1, obtained from fresh leaves and roots. For biological, lettuce root meristems were used for the preparation of slides using the technique of squashing. All blades were observed with an optical microscope at a magnitude of 400x. A total of 5,000 cells were analyzed for each treatment, and the number of cells in each phase of mitosis was recorded. Possible presence of chromosomal abnormalities was verified, such as chromatid breaks, anafasic bridges, loss of whole chromosomes or micronuclei formation. These analyzes were conducted only on a qualitative level. Results showed that aqueous extracts of M. lathyroides mitotic caused reduced index with increased concentration. Genotoxic activity was also observed for both extracts tested, since composition resulted in cell cycle changes and chromosomal abnormalities.

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