Secretome characterization of the lignocellulose-degrading fungi Pycnoporus sanguineus and Ganoderma resinaceum growing on Panicum prionitis biomass

ABSTRACTC4 grasses are common species in rangelands around the world and represent an attractive option for second-generation biofuels production. Although they display a high polysaccharide content and reach great levels of biomass accumulation, there is a major technical issue to be solved before they can be considered as biofuels feedstock: lignin removal. Concerning this, Pycnoporus and Ganoderma fungal genera have been highlighted due to their ability to hydrolyze lignocellulose. The goals here were to evaluate the pretreatment efficiency using P. sanguineus and G. applanatum secretomes harvested from a glucose-free inductive medium and to identify the fungal enzymatic activities responsible for the lignin degradation and glucose release. The findings show that P. sanguineus secretome exhibits a higher activity of lignocellulolytic enzymes compared to the one from G. applanatum. Interestingly, zymograms in presence of glucose suggest that a β-glucosidase isoform from P. sanguineus could be glucose-tolerant. The proteomic approach carried out allowed to identify 73 and 180 different proteins for G. applanatum and P. sanguineus secretomes, respectively, which were functionally classified in five main categories, and a miscellaneous group. Many uncharacterized proteins were found in both secretomes, reflecting that greater research is still needed for a better comprehension of lignocellulose degradation.

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Fabrication and Characterization of a Low-Cost Microfluidic System for the Manufacture of Alginate–Lacasse Microcapsules

Polymers ◽

10.3390/polym12051158 ◽

2020 ◽

Vol 12 (5) ◽

pp. 1158 ◽

Cited By ~ 4

Author(s):

Ana Lucia Campaña ◽

Diana Camila Sotelo ◽

Hector Alfonso Oliva ◽

Andres Aranguren ◽

Nancy Ornelas-Soto ◽

...

Keyword(s):

Fluorescent Dyes ◽

Low Cost ◽

Microfluidic System ◽

Universal Serial Bus ◽

Final Particle ◽

Pycnoporus Sanguineus ◽

Fluid Behavior ◽

Multiphysics Simulations ◽

Capsule Size

The development of microfluidics-based systems in the recent years has provided a rapid and controlled method for the generation of monodisperse microencapsulates for multiple applications. Here, we explore the design, manufacture and characterization of a low-cost microsystem for the encapsulation of the fungal laccase from Pycnoporus sanguineus CS43 in alginate microcapsules. Multiphysics simulations were used to overview the fluid behavior within the device and estimate the resulting capsule size. Polymethylmethacrylate (PMMA) sheets were used for final microsystem manufacture. Different flow rates of the continuous (Qc) and discrete (Qd) phases in the ranges of 83–293 mL/h and 1–5 mL/h, respectively, were evaluated for microcapsule fabrication. Universal Serial Bus (USB) microscope and image analysis was used to measure the final particle size. Laccase encapsulation was evaluated using spectrophotometry and with the aid of fluorescent dyes and confocal microscopy. Results showed microcapsule size was in the range of 203.13–716.00 μm and Qc was found as the dominant parameter to control capsule size. There was an effective enzyme encapsulation of 65.94% with respect to the initial laccase solution.

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