Spore viability and germination of some ectomycorrhizal fungi from New Zealand and implications for forest restoration

2021 ◽  
pp. 1-17
Author(s):  
Julia Bohorquez ◽  
Andy R. Nilsen ◽  
Matthew J. Larcombe ◽  
David A. Orlovich ◽  
Janice M. Lord
Keyword(s):  
New Zealand ◽  
Ectomycorrhizal Fungi ◽  
Forest Restoration ◽  
Spore Viability

scholarly journals Back to Roots: The Role of Ectomycorrhizal Fungi in Boreal and Temperate Forest Restoration

2020 ◽  
Vol 3 ◽  
Author(s):  
Nahuel Policelli ◽  
Thomas R. Horton ◽  
Aimée T. Hudon ◽  
Taylor R. Patterson ◽  
Jennifer M. Bhatnagar
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Forest Restoration ◽  
Temperate Forest

scholarly journals Detecting and identifying ectomycorrhizal fungi in New Zealand silver beech (Lophozonia menziesii, Nothofagaceae) forest: a case study

2018 ◽  
Vol 2 ◽  
pp. e25982
Author(s):  
David Orlovich ◽  
Andy Nilsen ◽  
Suliana Teasdale ◽  
Paul Guy ◽  
Tina Summerfield
Keyword(s):  
New Zealand ◽  
Community Composition ◽  
Ectomycorrhizal Fungi ◽  
Dna Sequences ◽  
Amplicon Sequencing ◽  
Fungal Species ◽  
Study Data ◽  
Native Forest ◽  
Canopy Soil

Adventitious roots in canopy soils associated with silver beech (Lophozonia menziesii (Hook.f.) Heenan & Smissen (Nothofagaceae)) form ectomycorrhizal associations. We used amplicon sequencing of the internal transcribed spacer 2 region to compare diversity of ectomycorrhizal fungal species in canopy and terrestrial sites. The study data are archived as an NCBI BioProject (accession PRJNA421209), with the raw DNA sequence reads available from the NCBI Sequence Read Archive SRA637723 Community composition of canopy ectomycorrhizal fungi was significantly different to the terrestrial community composition, with several abundant ectomycorrhizal species significantly more represented in the terrestrial soil than the canopy soil. Additionally, we found evidence that an introduced ectomycorrhizal species was present in these native forest soils. We identified OTUs in two ways: (i) by manually curated BLAST searching of the NCBI nr database, and (ii) by comparison with Species Hypotheses on UNITE v.7.2. We desired to make species identifications where we could be reasonably confident they were robust, but had to avoid making identifications when an incorrect name could have implications for biosecurity or our understanding of biodiversity and biogeography. We found some UNITE Species Hypotheses included sequences of more than one taxon, which we were able to separate and distinguish by phylogenetic analysis. Consequently we exercised caution in reporting names based on the Species Hypotheses. Using data from this case study, we will illustrate the achievements and challenges faced in identifying species of ectomycorrhizal fungi from DNA barcodes. Most DNA sequences of ectomycorrhizal fungi matched closely New Zealand voucher specimens stored in either the New Zealand Fungal Herbarium (PDD) or the Otago Regional Herbarium (OTA), which facilitated the validation of identifications. In the case of PDD specimens, collection and DNA data were linked via the Systematics Collections Data database (https://scd.landcareresearch.co.nz). We are working towards a similar database for OTA specimens, using the Specify 6 database platform.

scholarly journals Spatial Distribution of the Mexican Daisy, Erigeron karvinskianus, in New Zealand under Climate Change

Climate ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 24 ◽  
Author(s):  
Lauren Hannah ◽  
Glenn Aguilar ◽  
Dan Blanchon
Keyword(s):  
Spatial Distribution ◽  
New Zealand ◽  
Species Distribution ◽  
Forest Restoration ◽  
Greenhouse Gas Emission ◽  
Species Distribution Modeling ◽  
Climatic Conditions ◽  
Native Forest ◽  
Climate Conditions ◽  
Distribution Modeling

The invasive species Erigeron karvinskianus or Mexican daisy is considered a significant weed that impacts native forest restoration efforts in New Zealand. Mapping the potential distribution of this species under current and future predicted climatic conditions provides managers with relevant information for developing appropriate management strategies. Using occurrences available from global and local databases, spatial distribution characteristics were analyzed using geostatistical tools in ArcMap to characterize current distribution. Species distribution modeling (SDM) using Maxent was conducted to determine the potential spatial distribution of E. karvinskianus worldwide and in New Zealand with projections into future climate conditions. Potential habitat suitability under future climatic conditions were simulated using greenhouse gas emission trajectories under the Representative Concentration Pathway (RCP) models RCP2.6, RCP4.5, RCP6.0 and RCP8.5 for years 2050 and 2070. Occurrence data were processed to minimize redundancy and spatial autocorrelation; non-correlated environmental variables were determined to minimize bias and ensure robust models. Kernel density, hotspot and cluster analysis of outliers show that populated areas of Auckland, Wellington and Christchurch have significantly greater concentrations of E. karvinskianus. Species distribution modeling results find an increase in the expansion of range with higher RCP values, and plots of centroids show a southward movement of predicted range for the species.

Magellanic Cloud Eclipsing Binaries: Primary Distance Indicators

1999 ◽  
Vol 190 ◽  
pp. 563-566
Author(s):  
J. D. Pritchard ◽  
W. Tobin ◽  
J. V. Clausen ◽  
E. F. Guinan ◽  
E. L. Fitzpatrick ◽  
...  
Keyword(s):  
New Zealand ◽  
Magellanic Cloud ◽  
Eclipsing Binaries ◽  
Fundamental Parameters ◽  
Distance Indicators

Our collaboration involves groups in Denmark, the U.S.A. Spain and of course New Zealand. Combining ground-based and satellite (IUEandHST) observations we aim to determine accurate and precise stellar fundamental parameters for the components of Magellanic Cloud Eclipsing Binaries as well as the distances to these systems and hence the parent galaxies themselves. This poster presents our latest progress.

Heat-Etching with a Bullivant Type II Simple Freeze-Cleave Device

1970 ◽  
Vol 28 ◽  
pp. 106-107 ◽  
Author(s):  
Ronald S. Weinstein ◽  
N. Scott McNutt
Keyword(s):  
New Zealand ◽  
General Hospital ◽  
Massachusetts General Hospital ◽  
Type I ◽  
Type Ii ◽  
Collaborative Effort ◽  
Temperature And Pressure ◽  
The University

The Type I simple cold block device was described by Bullivant and Ames in 1966 and represented the product of the first successful effort to simplify the equipment required to do sophisticated freeze-cleave techniques. Bullivant, Weinstein and Someda described the Type II device which is a modification of the Type I device and was developed as a collaborative effort at the Massachusetts General Hospital and the University of Auckland, New Zealand. The modifications reduced specimen contamination and provided controlled specimen warming for heat-etching of fracture faces. We have now tested the Mass. General Hospital version of the Type II device (called the “Type II-MGH device”) on a wide variety of biological specimens and have established temperature and pressure curves for routine heat-etching with the device.

A Cytochemical Study of Glucose-6-Phosphatase (G-6-PASE) in Cells Participating in Atherogenesis of Rabbit Aorta

1975 ◽  
Vol 33 ◽  
pp. 460-461
Author(s):  
Sidney D. Kobernick ◽  
Edna A. Elfont ◽  
Neddra L. Brooks
Keyword(s):  
Lipid Metabolism ◽  
New Zealand ◽  
Aortic Wall ◽  
Rabbit Aorta ◽  
Plaque Formation ◽  
Metabolic Changes ◽  
Atherosclerotic Plaques ◽  
Cytochemical Study ◽  
Cellular Alteration ◽  
New Zealand White Rabbits

This cytochemical study was designed to investigate early metabolic changes in the aortic wall that might lead to or accompany development of atherosclerotic plaques in rabbits. The hypothesis that the primary cellular alteration leading to plaque formation might be due to changes in either carbohydrate or lipid metabolism led to histochemical studies that showed elevation of G-6-Pase in atherosclerotic plaques of rabbit aorta. This observation initiated the present investigation to determine how early in plaque formation and in which cells this change could be observed.Male New Zealand white rabbits of approximately 2000 kg consumed normal diets or diets containing 0.25 or 1.0 gm of cholesterol per day for 10, 50 and 90 days. Aortas were injected jin situ with glutaraldehyde fixative and dissected out. The plaques were identified, isolated, minced and fixed for not more than 10 minutes. Incubation and postfixation proceeded as described by Leskes and co-workers.

Sign in / Sign up

Export Citation Format

Share Document