Effect of molecular weight and polymer concentration on the triple temperature/pH/ionic strength-sensitive behavior of poly(2-(dimethylamino)ethyl methacrylate)

2017 ◽  
Vol 66 (9) ◽  
pp. 455-461 ◽  
Author(s):  
Maryam Mohammadi ◽  
Mehdi Salami-Kalajahi ◽  
Hossein Roghani-Mamaqani ◽  
Marzieh Golshan
1987 ◽  
Vol 58 (04) ◽  
pp. 1064-1067 ◽  
Author(s):  
K Kodama ◽  
B Pasche ◽  
P Olsson ◽  
J Swedenborg ◽  
L Adolfsson ◽  
...  

SummaryThe mode of F Xa inhibition was investigated on a thromboresistant surface with end-point attached partially depoly-merized heparin of an approximate molecular weight of 8000. Affinity chromatography revealed that one fourth of the heparin used in surface coating had high affinity for antithrombin III (AT). The heparin surface adsorbed AT from both human plasma and solutions of purified AT. By increasing the ionic strength in the AT solution the existence of high and low affinity sites could be shown. The uptake of AT was measured and the density of available high and low affinity sites was found to be in the range of 5 HTid 11 pic.omoles/cmf, respectively Thus the estimated density of biologically active high and low ailmity heparm respectively would be 40 and 90 ng/cm2 The heparin coating did not take up or exert F Xa inhibition by itself. With AT adsorbed on both high and low affinity heparin the surface had the capacity to inhibit several consecutive aliquots of F Xa exposed to the surface. When mainly high affinity sites were saturated with AT the inhibition capacity was considerably lower. Tt was demonstrated that the density of AT on both high and low affinity heparin determines the F Xa inhibition capacity whereas the amount of AT on high affinity sites limits the rate of the reaction. This implies that during the inhibition of F Xa there is a continuous surface-diffusion of AT from sites of a lower class to the high affinity sites where the F Xa/AT complex is formed and leaves the surface. The ability of the immobilized heparin to catalyze inhibition of F Xa is likely to be an important component for the thromboresistant properties of a heparin coating with non-compromized AT binding sequences.


1993 ◽  
Vol 70 (05) ◽  
pp. 867-872 ◽  
Author(s):  
Dingeman C Rijken ◽  
Gerard A W de Munk ◽  
Annie F H Jie

SummaryIn order to define the possible effects of heparin on the fibrinolytic system under physiological conditions, we studied the interactions of this drug with plasminogen and its activators at various ionic strengths. As reported in recent literature, heparin stimulated the activation of Lys-plasminogen by high molecular weight (HMW) and low molecular weight (LMW) two-chain urokinase-type plasminogen activator (u-PA) and two-chain tissue-type plasminogen activator (t-PA) 10- to 17-fold. Our results showed, however, that this stimulation only occurred at low ionic strength and was negligible at a physiological salt concentration. Direct binding studies were performed using heparin-agarose column chromatography. The interaction between heparin and Lys-plasminogen appeared to be salt sensitive, which explains at least in part why heparin did not stimulate plasminogen activation at 0.15 M NaCl. The binding of u-PA and t-PA to heparinagarose was less salt sensitive. Results were consistent with heparin binding sites on both LMW u-PA and the amino-terminal part of HMW u-PA. Single-chain t-PA bound more avidly than two-chain t-PA. The interactions between heparin and plasminogen activators can occur under physiological conditions and may modulate the fibrinolytic system.


1973 ◽  
Vol 30 (01) ◽  
pp. 093-105 ◽  
Author(s):  
C.H.J Sear ◽  
L Poller ◽  
F.R.C Path

SummaryThe antiheparin activity of normal serum has been studied by comparing the antiheparin activities of sera obtained from normal whole blood, platelet-rich plasma and platelet-’free’ plasma with a purified platelet extract during differential isoelectric precipitation and by gel filtration chromatography.The mean values for the activity of PRP-serum and PFP-serum were 106% (S.D. 11) and 10% (S.D. 3) of untreated whole blood respectively. The activity of whole blood serum, PRP serum and whole blood serum plus platelet extract precipitated under identical physical conditions, i.e. pH 7.0, I =0.008, indicating that the activities of the three samples are probably associated with PF4. PF4 precipitated from human platelet extract at pH 4.0, but this is probably due to the difference in the two biochemical environments investigated, i.e. serum and platelet extract.The gel filtration experiments revealed striking similarities between the major antiheparin activities of serum and platelet extract. At physiological pH and ionic strength both activities were associated with high molecular weight material, but at physiological pH and elevated ionic strength both activities behaved as much smaller entities of molecular weight between 25,000 and 30,000 daltons and it seems very likely that both activities are associated with the same molecule, i.e. PF4.


2017 ◽  
Vol 812 ◽  
pp. 1076-1118 ◽  
Author(s):  
S. S. Srinivas ◽  
V. Kumaran

The modification of soft-wall turbulence in a microchannel due to small amounts of polymer dissolved in water is experimentally studied. The microchannels are of rectangular cross-section with height ${\sim}$160 $\unicode[STIX]{x03BC}\text{m}$, width ${\sim}$1.5 mm and length ${\sim}$3 cm, with three walls made of hard polydimethylsiloxane (PDMS) gel, and one wall made of soft PDMS gel with an elasticity modulus of ${\sim}$18 kPa. Solutions of polyacrylamide of molecular weight $5\times 10^{6}$ and mass fraction up to 50 ppm, and of molecular weight $4\times 10^{4}$ and mass fraction up to 1500 ppm, are used in the experiments. In all cases, the solutions are in the dilute limit below the critical overlap concentration, and the solution viscosity does not exceed that of water by more than 10 %. Two distinct types of flow modifications are observed below and above a threshold mass fraction for the polymer, $w_{t}$, which is ${\sim}$1 ppm and 500 ppm for the solutions of polyacrylamide with molecular weights $5\times 10^{6}$ and $4\times 10^{4}$, respectively. At or below $w_{t}$, there is no change in the transition Reynolds number, but there is significant turbulence attenuation, by up to a factor of 2 in the root-mean-square velocities and a factor of 4 in the Reynolds stress. When the polymer concentration increases beyond $w_{t}$, there is a decrease in the transition Reynolds number and in the intensity of the turbulent fluctuations. The lowest transition Reynolds number is ${\sim}$35 for the solution of polyacrylamide with molecular weight $5\times 10^{6}$ and mass fraction 50 ppm (in contrast to 260–290 for pure water). The fluctuating velocities in the streamwise and cross-stream directions are lower by a factor of 5, and the Reynolds stress is lower by a factor of 10, in comparison to pure water.


Soft Matter ◽  
2021 ◽  
Author(s):  
Athena E. Metaxas ◽  
Vishal Panwar ◽  
Ruth L. Olson ◽  
Cari S. Dutcher

A Taylor–Couette cell capable of radial injection was used to study the effects of varying solution ionic strength and polyelectrolyte molecular weight on the polyelectrolyte-driven flocculation of bentonite suspensions.


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