A study of the prevalence of hydrogen peroxide generatingLactobacilliin bacterial vaginosis: the determination of H2O2concentrations generated,in vitro, by isolated strains and the levels found in vaginal secretions of women with and without infection

1998 ◽  
Vol 18 (1) ◽  
pp. 63-67 ◽  
Author(s):  
S. AL-MUSHRIF B. M. JONES
Keyword(s):  
Hydrogen Peroxide ◽  
Bacterial Vaginosis ◽  
Vaginal Secretions

scholarly journals Determination of an effective method of Citrus aurantifolia lime and Citrus lemon varieties plant explants sterilization for in vitro introduction into the culture

2020 ◽  
pp. 185-191
Author(s):  
S. Shokh ◽  
Z. Sych ◽  
L. Karpuk
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Hypochlorite ◽  
Ethanol Solution ◽  
In Vitro Cultivation ◽  
Citrus Aurantifolia ◽  
Planting Material ◽  
Mother Plants ◽  
Cultivation Techniques

Potted citrus plants are in constant demand, but the propagation of such plants requires the use of a winter greenhouse and several well-developed mother plants to obtain planting material. The use of microclonal propagation can accelerate the production of seed material though it requires detailed development of in vitro cultivation techniques. The aim of the research was to study the effectiveness of sterilizing substances and methods of sterilization on the yield of viable micro shoots of lime plants (Citrus aurantifolia) as well as Meyer and Jubilee lemon varieties and their growth in vitro. The research was conducted in the interdepartmental "Biotechnological Laboratory" of the Agrobiotechnological Faculty of Bila Tserkva NAU. The source material was microshoots from lime plants (Citrus aurantifolia), and lemon varieties (Citrus lemon) Meyer and Jubilee. The objects of the study were selected according to the diversity of genotypes, development type and varietal characteristics. The possibility of using different parts of plants for reproduction is a feature of in vitro culture. We used microshoots with a bud in our research. To neutralize the exogenous bacterial and fungal microflora, we used 70 % ethanol solution C2 H5 OH, sodium hypochlorite 5 %, 15 % solution of hydrogen peroxide H2 O2 , sulema (0.1) and washed the material in sterile water (for 5–10 min) under several schemes. The study revealed the influence of different sterilization regimes for obtaining sterile explants and the introduction into plant culture of explants of the species of lime Citrus aurantifolia as well as Meyer and Jubilee lemon varieties. It was found that the use of step sterilization using 2.5 % solutions of sodium hypochlorite gives a high percentage of sterile viable material – 27.7 %, which is significantly higher than in other variants of the experiment. Key words: microshoots, lime, sterile material, micropropagation, sterilization, hydrogen peroxide, viability of explants.

Catecholamine interference with enzymatic determination of nonesterified fatty acids in two commercially available test kits

1995 ◽  
Vol 41 (3) ◽  
pp. 455-457 ◽  
Author(s):  
R E Carr ◽  
S M Humphreys ◽  
K N Frayn
Keyword(s):  
Hydrogen Peroxide ◽  
Fatty Acids ◽  
Adipose Tissue ◽  
Nonesterified Fatty Acids ◽  
Present Evidence ◽  
Adipose Tissue In Vitro ◽  
Enzymatic Determination ◽  
Test Kit

Abstract We present evidence that catecholamines, which are commonly used to stimulate lipolysis in adipose tissue in vitro, interfere with the enzymatic determination of non-esterified fatty acid (NEFA) in two commercially available kits. Measurement of a 100 mumol/L standard with the Wako "NEFA C" test kit was 60% inhibited by 100 mumol/L norepinephrine and was completely inhibited by 100 mumol/L isoproterenol or by 1 mmol/L norepinephrine or epinephrine. Measurement with the Boehringer Mannheim "Free Fatty acids, Half-micro test" was completely inhibited by 100 mumol/L norepinephrine and was also affected by concentrations as low as 0.1 mumol/L. We propose that this effect is due to the catecholamines interfering with a step common to the two kits, the generation of hydrogen peroxide and oxidation of a chromagen; furthermore, this interference appears to be stoichiometric. We also give details of an alternative in-house method, which does not depend on the generation of hydrogen peroxide and is not affected by catecholamines.

Hydrogen peroxide, antioxidant compounds and biological targets: An in vitro approach for determination of scavenging capacity using fluorimetric multisyringe flow injection analysis

Talanta ◽  
2010 ◽  
Vol 81 (4-5) ◽  
pp. 1840-1846 ◽  
Author(s):  
Joana P.N. Ribeiro ◽  
Luís M. Magalhães ◽  
Marcela A. Segundo ◽  
Salette Reis ◽  
José L.F.C. Lima
Keyword(s):  
Hydrogen Peroxide ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Antioxidant Compounds ◽  
Biological Targets ◽  
Multisyringe Flow Injection Analysis ◽  
Scavenging Capacity

scholarly journals Probiotic Lactobacillus sp. Strains Inhibit Growth, Adhesion, Biofilm Formation, and Gene Expression of Bacterial Vaginosis-Inducing Gardnerella vaginalis

2021 ◽  
Vol 9 (4) ◽  
pp. 728
Author(s):  
Zhixiang Qian ◽  
Hui Zhu ◽  
Dan Zhao ◽  
Ping Yang ◽  
Fei Gao ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Bacterial Vaginosis ◽  
Biofilm Formation ◽  
Culture Supernatant ◽  
Short Chain Fatty Acids ◽  
Lactobacillus Delbrueckii ◽  
Gardnerella Vaginalis ◽  
Expression Of Genes ◽  
Suspended Cells

Gardnerella vaginalis contributes significantly to bacterial vaginosis, which causes an ecological imbalance in vaginal microbiota and presents with the depletion of Lactobacillus sp. Lactobacillus supplementation was reported to be an approach to treat bacterial vaginosis. We investigated the applicability of three Lactobacillus sp. strains (Lactobacillus delbrueckii DM8909, Lactiplantibacillus plantarum ATCC14917, and Lactiplantibacillus plantarum ZX27) based on their probiotic abilities in vitro. The three candidate Lactobacillus sp. strains for bacterial vaginosis therapy showed distinct properties in auto-aggregation ability, hydrophobicity, adhesion to cervical epithelial cells, and survivability in 0.01% hydrogen peroxide. Lpb. plantarum ZX27 showed a higher yield in producing short-chain fatty acids and lactic acid among the three candidate strains, and all three Lactobacillus sp. strains inhibited the growth and adhesion of G. vaginalis. Furthermore, we discovered that the culture supernatant of Lactobacillus sp. exhibited anti-biofilm activity against G. vaginalis. In particular, the Lpb. plantarum ZX27 supernatant treatment decreased the expression of genes related to virulence factors, adhesion, biofilm formation, metabolism, and antimicrobial resistance in biofilm-forming cells and suspended cells. Moreover, Lactobacillus sp. decreased the upregulated expression of interleukin−8 in HeLa cells induced by G. vaginalis or hydrogen peroxide. These results demonstrate the efficacy of Lactobacillus sp. application for treating bacterial vaginosis by limiting the growth, adhesion, biofilm formation, and virulence properties of G. vaginalis.

Bactericidal properties of peracetic acid and hydrogen peroxide, alone and in combination, and chlorine and formaldehyde against bacterial water strains

1992 ◽  
Vol 38 (7) ◽  
pp. 635-642 ◽  
Author(s):  
Anouar Alasri ◽  
Christine Roques ◽  
Georges Michel ◽  
Corinne Cabassud ◽  
Philippe Aptel
Keyword(s):  
Hydrogen Peroxide ◽  
Peracetic Acid ◽  
Culture Collection ◽  
Minimal Bactericidal Concentration ◽  
Ultrafiltration Membranes ◽  
Bactericidal Properties ◽  
Rapid Destruction ◽  
Bactericidal Activities

The bactericidal properties of peracetic acid, hydrogen peroxide, chlorine, and formaldehyde were compared in vitro using a rapid micromethod. A combination of peracetic acid and hydrogen peroxide was also tested to assess interactions. The activities of these agents, which are widely used as disinfectants, were evaluated against water isolates and culture collection strains. Peracetic acid and chlorine exhibited an excellent antimicrobial activity, with a relatively rapid destruction of 105 bacteria/mL. The time-dependent bactericidal activities of hydrogen peroxide and formaldehyde were the lowest. The combination of peracetic acid and hydrogen peroxide, tested by a checkerboard micromethod, was found to be synergistic. The minimal bactericidal concentration was established in terms of time for a given mixture of peracetic acid and hydrogen peroxide. Determination of bactericidal concentrations showed that synergy was maintained with increasing contact time. Concentrations for minimal times of treatment by chemicals that provided interesting activities in vitro were tested for disinfection of ultrafiltration membranes. The bactericidal activities of peroxygen compounds were confirmed and synergism was maintained in working conditions. Chlorine showed a loss of efficacy when used on membranes. Key words: peracetic acid, hydrogen peroxide, chlorine, formaldehyde, minimal bactericidal concentration, ultrafiltration membranes.

Selective LC determination of cabergoline in the bulk drug and in tablets: In vitro dissolution studies

2007 ◽  
Vol 65 (9-10) ◽  
pp. 561-567
Author(s):  
A ONAL ◽  
O SAGIRLI ◽  
D SENSOY
Keyword(s):  
In Vitro Dissolution ◽  
Dissolution Studies ◽  
Bulk Drug

Determination of in vitro „anti-aging„-effects of Ganoderma pfeifferi

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
W Jülich ◽  
J Pörksen ◽  
H Welzel ◽  
U Lindequist
Keyword(s):  
Aging Effects

In vitro determination of the skin anti-aging potential of eleven South African plants

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
GN Ndlovu ◽  
G Fouche ◽  
W Cordier ◽  
V Steenkamp ◽  
M Tselanyane
Keyword(s):  
South African ◽  
South African Plants ◽  
African Plants
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