A Device for Countercurrent Distribution of Particles by an Aqueous Polymer Two-Phase System

Capsaicin, which mainly comes from pepper, exhibits anticancer, antioxidant, and anti-obesity properties. This work aims to construct a comprehensive technology for the extraction and purification of capsaicin from capsicum oleoresin. The tunable aqueous polymer phase impregnated HZ816 resins were selected in extraction step. In the extraction process, 3 g of impregnated HZ816 macroporous resin was employed per system. The results showed that a higher molecular weight of Polyethylene glycol (PEG) and 1-ethyl-3-methyl imidazolium acetate ([Emim] [OAc]) are more beneficial to the improvement of the yield of capsaicin. Screening experiment using fractional factorial designs indicated that the amount of sample loading, pH, and concentration of [Emim] [OAc] and PEG 6000 significantly affect the yield of capsaicin. Mathematical models of capsaicin yield in tunable aqueous polymer-phase impregnated resins were established and optimum condition was obtained using response surface methodology. The optimum impregnated phase was the polymer phase of an aqueous two-phase system which contained 18.5% (w/w) PEG6000, 15% (w/w) sodium citrate, and 10% (w/w) [Emim] [OAc] at pH 6.5. Under the optimal conditions, the yield of capsaicin reached 95.82% when the extraction system contains 0.25 g capsicum oleoresin. Ultimately, capsaicinoids extract was purified by reverse-phase resin (SKP-10-4300) chromatographic column. The capsaicin recovery and purity achieved 85% and 92%, respectively.

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Isolation of Plasma Membranes from the Nervous System by Countercurrent Distribution in Aqueous Polymer Two-Phase Systems

Proteomics - Methods in Molecular Biology™ ◽

10.1007/978-1-60761-157-8_19 ◽

2009 ◽

pp. 335-340 ◽

Cited By ~ 1

Author(s):

Jens Schindler ◽

Hans Gerd Nothwang

Keyword(s):

Nervous System ◽

Plasma Membranes ◽

Countercurrent Distribution ◽

Two Phase ◽

Aqueous Polymer ◽

Phase Systems

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PREPARATION AND CHARACTERIZATION OF INTACT AND FREE SPORES OF BACILLUS POPILLIAE DUTKY

Canadian Journal of Microbiology ◽

10.1139/m67-036 ◽

1967 ◽

Vol 13 (3) ◽

pp. 279-285 ◽

Cited By ~ 13

Author(s):

Grant St. Julian ◽

Thomas G. Pridham ◽

Harlow H. Hall

Keyword(s):

Malachite Green ◽

Potassium Phosphate ◽

Staining Technique ◽

Phase System ◽

Two Phase ◽

Aqueous Polymer ◽

Sonic Treatment ◽

Bacillus Popilliae ◽

Almost All

Spores of Bacillus popilliae are enclosed within a large sporangium. In an effort to characterize the spores precisely, a method was developed to free them from their sporangia by sonic vibration. Separation of the free spores from sporangial debris was made by lysozyme treatment, centrifugation, and the Sacks and Alderton aqueous polymer two-phase system. The intact spores are fully refractile; they accept and retain malachite green applied by a modified Wirtz spore-staining technique. The free spores are elongated with rounded ends and measure 1.30–2.25 μ × 0.90–1.40 μ. Outgrowth of intact germinated spores is lowest when heat-activated at 80 °C and highest when heat-activated at 50 °C. Free spores are more heat sensitive than are intact spores, possibly because of damage by the sonic treatment. L-Alanine, adenosine, and glucose separately and in combination do not influence germination of either intact or free spores. Although potassium phosphate somewhat increased germination of intact spores, other salts were ineffective, but almost all salts increased germination of free spores.

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