scholarly journals Extraction and Purification of Capsaicin from Capsicum Oleoresin Using a Combination of Tunable Aqueous Polymer-Phase Impregnated Resin (TAPPIR) Extraction and Chromatography Technology

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3956 ◽  
Author(s):  
Lu ◽  
Cui
Keyword(s):  
Sodium Citrate ◽  
Extraction Process ◽  
Fractional Factorial ◽  
Phase System ◽  
Polymer Phase ◽  
Macroporous Resin ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Extraction And Purification ◽  
Extraction Step

Capsaicin, which mainly comes from pepper, exhibits anticancer, antioxidant, and anti-obesity properties. This work aims to construct a comprehensive technology for the extraction and purification of capsaicin from capsicum oleoresin. The tunable aqueous polymer phase impregnated HZ816 resins were selected in extraction step. In the extraction process, 3 g of impregnated HZ816 macroporous resin was employed per system. The results showed that a higher molecular weight of Polyethylene glycol (PEG) and 1-ethyl-3-methyl imidazolium acetate ([Emim] [OAc]) are more beneficial to the improvement of the yield of capsaicin. Screening experiment using fractional factorial designs indicated that the amount of sample loading, pH, and concentration of [Emim] [OAc] and PEG 6000 significantly affect the yield of capsaicin. Mathematical models of capsaicin yield in tunable aqueous polymer-phase impregnated resins were established and optimum condition was obtained using response surface methodology. The optimum impregnated phase was the polymer phase of an aqueous two-phase system which contained 18.5% (w/w) PEG6000, 15% (w/w) sodium citrate, and 10% (w/w) [Emim] [OAc] at pH 6.5. Under the optimal conditions, the yield of capsaicin reached 95.82% when the extraction system contains 0.25 g capsicum oleoresin. Ultimately, capsaicinoids extract was purified by reverse-phase resin (SKP-10-4300) chromatographic column. The capsaicin recovery and purity achieved 85% and 92%, respectively.

Ultrasonic-Assisted Aqueous Two-Phase Extraction Combined with Macroporous Resin Enrichment of Lignans from Flaxseed Meal and Their Antioxidant Activities

2021 ◽  
Author(s):  
Zhizhou Zhang ◽  
Yongchao Zhang ◽  
Yichao Wu ◽  
Yongxiang Xu ◽  
Yuanyuan Jiang ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Phase System ◽  
Extraction Temperature ◽  
Macroporous Resin ◽  
Two Phase ◽  
Solid Ratio ◽  
Flaxseed Meal ◽  
Extraction And Purification ◽  
Ultrasonic Assisted

Abstract Background Secoisolariciresinol di glucoside (SDG) is a natural antioxidant generally extracted from flaxseed, which is one of the most important oil crops in China, the by-product of the flaxseed oil, i.e., flaxseed meal, still contains a lot of lignans. However, flaxseed meal is generally treated as waste, resulting in a huge waste of resources. Objective To establish an efficient and convenient method for extraction and purification of lignans from flaxseed meal. Methods First, we used response surface methodology (RSM) to optimize the extraction conditions of the ultrasonic-assisted aqueous two-phase system (UAATPS), and we obtained the purified extracts by macroporous resin purification (MRP). Second, the antioxidant ability of the extracts was studied in vitro. Results The best extraction conditions obtained were as follows: 9.0 wt% sodium hydroxide, 30.0 wt% isopropanol, extraction time 39 min, liquid-to-solid ratio of 52.0 mL/g, ultrasonic wave 560 W, and extraction temperature 40 °C. Under the optimal conditions, the purity of crude extracts (SDG-APTS-C) reached 21.5%. The desorption conditions of MRP were as follows: eluting 3 BV with ultrapure water, and then eluting with 25% ethanol at 2 BV/h to collect eluents. The purified extracts (SDG-ATPS-P) had a purity quotient of 73.9%, 52.4% higher than that of SDG-ATPS-C. Additionally, experiments conducted in this paper revealed that SDG-ATPS-C and SDG-ATPS-P could effectively remove DPPH, ABTS, and hydroxyl free radicals in vitro. Conclusions The method was validated for extracting SDG from flaxseed meal, thus achieving the reuse of flaxseed meal. Highlights This research provides some references for the application of UAATPS combined with MRP in natural products.

scholarly journals Partitioning of bovine lactoferrin in aqueous two-phase system containing poly(ethylene glycol) and sodium citrate

2015 ◽  
Vol 95 ◽  
pp. 118-124 ◽  
Author(s):  
Angélica Ribeiro da Costa ◽  
Jane Sélia dos Reis Coimbra ◽  
Luísa Alexandre Ferreira ◽  
João Carlos Marcos ◽  
Igor José Boggione Santos ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Sodium Citrate ◽  
Bovine Lactoferrin ◽  
Phase System ◽  
Poly Ethylene Glycol ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Poly Ethylene

scholarly journals Purification of alkaline protease from chicken intestine by aqueous two phase system of polyethylene glycol and sodium citrate

2010 ◽  
Vol 48 (1) ◽  
pp. 36-44 ◽  
Author(s):  
B. K. Sarangi ◽  
D. P. Pattanaik ◽  
K. Rathinaraj ◽  
N. M. Sachindra ◽  
M. C. Madhusudan ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Alkaline Protease ◽  
Sodium Citrate ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Chicken Intestine

PREPARATION AND CHARACTERIZATION OF INTACT AND FREE SPORES OF BACILLUS POPILLIAE DUTKY

1967 ◽  
Vol 13 (3) ◽  
pp. 279-285 ◽  
Author(s):  
Grant St. Julian ◽  
Thomas G. Pridham ◽  
Harlow H. Hall
Keyword(s):  
Malachite Green ◽  
Potassium Phosphate ◽  
Staining Technique ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Sonic Treatment ◽  
Bacillus Popilliae ◽  
Almost All

Spores of Bacillus popilliae are enclosed within a large sporangium. In an effort to characterize the spores precisely, a method was developed to free them from their sporangia by sonic vibration. Separation of the free spores from sporangial debris was made by lysozyme treatment, centrifugation, and the Sacks and Alderton aqueous polymer two-phase system. The intact spores are fully refractile; they accept and retain malachite green applied by a modified Wirtz spore-staining technique. The free spores are elongated with rounded ends and measure 1.30–2.25 μ × 0.90–1.40 μ. Outgrowth of intact germinated spores is lowest when heat-activated at 80 °C and highest when heat-activated at 50 °C. Free spores are more heat sensitive than are intact spores, possibly because of damage by the sonic treatment. L-Alanine, adenosine, and glucose separately and in combination do not influence germination of either intact or free spores. Although potassium phosphate somewhat increased germination of intact spores, other salts were ineffective, but almost all salts increased germination of free spores.

A Device for Countercurrent Distribution of Particles by an Aqueous Polymer Two-Phase System

1978 ◽  
Vol 13 (9) ◽  
pp. 745-752 ◽  
Author(s):  
Hiroyuki Nakazawa ◽  
Takenori Tanimura ◽  
Zenzo Tamura
Keyword(s):  
Countercurrent Distribution ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Polymer

Purification of glucosyltransferase from cell-lysate of Streptococcus mutans by counter-current chromatography using aqueous polymer two-phase system

2004 ◽  
Vol 805 (1) ◽  
pp. 155-160 ◽  
Author(s):  
Akio Yanagida ◽  
Mitsuhiro Isozaki ◽  
Yoichi Shibusawa ◽  
Heisaburo Shindo ◽  
Yoichiro Ito
Keyword(s):  
Streptococcus Mutans ◽  
Phase System ◽  
Two Phase ◽  
Cell Lysate ◽  
Aqueous Polymer ◽  
Counter Current
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