An explorative study comparing skin surface lipids in the West Highland white terrier dog with and without atopic dermatitis

Abstract Background – The skin barrier is important in the pathogenesis of atopic dermatitis and stratum corneum lipids have a critical role. Skin surface lipids have been largely overlooked but also contribute to barrier function. An untargeted approach was used to compare the skin surface lipids from atopic and non-atopic West Highland White terrier dogs. The primary hypothesis was that a difference in the lipidome of atopic and non-atopic dogs would be found and the secondary hypothesis was that affected and unaffected skin would differ in lipid profile.Results – Thirty-nine dogs were classified into one of four disease status groups based on strict criteria. Samples for lipid analysis were collected from affected and unaffected skin, and liquid chromatography/mass spectrometry found 421 lipid soluble features. Ten lipids were positively identified. Statistical analysis could not distinguish between non-atopic and atopic dogs. Partial least squares-discriminant analysis revealed a difference in the lipid profiles from affected and non-affected skin irrespective of disease status. Conclusions – An untargeted approach found a large array of unidentified lipids from the skin surface. There was a difference in the lipidome between affected and unaffected skin that was not related to disease status. Investigation into the lipidome of the skin surface in health and disease is an emerging area of research which could have clinical and therapeutic applications.

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Influence of Hot Spring Water on Fatty Acid Composition of Skin Surface Lipids in Hairless Mouse Model of Atopic Dermatitis

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.b16-00275 ◽

2016 ◽

Vol 39 (10) ◽

pp. 1718-1722 ◽

Cited By ~ 2

Author(s):

Kazuhiko Nishimura ◽

Naoki Aoyanagi ◽

Eiji Uchino ◽

Yutaka Itabashi

Keyword(s):

Atopic Dermatitis ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Mouse Model ◽

Hot Spring ◽

Skin Surface ◽

Hairless Mouse ◽

Spring Water ◽

Surface Lipids ◽

Skin Surface Lipids

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Non-invasive human skin transcriptome analysis using mRNA in skin surface lipids

10.1101/2021.04.04.438351 ◽

2021 ◽

Author(s):

Takayoshi Inoue ◽

Tetsuya Kuwano ◽

Yuya Uehara ◽

Michiko Yano ◽

Naoki Oya ◽

...

Keyword(s):

Atopic Dermatitis ◽

Transcriptome Analysis ◽

Skin Diseases ◽

Skin Surface ◽

Hair Follicles ◽

Sebaceous Glands ◽

Non Invasive ◽

Skin Physiology ◽

Surface Lipids ◽

Skin Surface Lipids

Non-invasive acquisition of mRNA data from the skin would be extremely useful for understanding skin physiology and diseases. Inspired by the holocrine process, in which the sebaceous glands secrete cell contents into the sebum, we focused on the possible presence of mRNAs in skin surface lipids (SSLs). We found that measurable human mRNAs exist in SSLs, where sebum protects them from degradation by RNases. The AmpliSeq transcriptome analysis was modified to measure SSL-RNAs, and our results revealed that SSL-RNAs predominantly contained mRNAs derived from sebaceous glands, epidermis, and hair follicles. Analysis of SSL-RNAs non-invasively collected from patients with atopic dermatitis revealed significantly increased expression of inflammation-related genes and decreased expression of terminal differentiation-related genes, consistent with the results of previous reports. Further, we found that lipid synthesis-related genes were downregulated in the sebaceous glands of patients with atopic dermatitis. These results indicate that the analysis of SSL-RNAs is promising to understand the pathophysiology of skin diseases.

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