Oxidative and genotoxic effects of bisphenol A on primary gill cell culture of Lake Van Fish (Alburnus tarichi Güldenstädt, 1814)

Abstract Lake Van fish (Alburnus tarichi Guldenstadt 1814) is the only fish that can adapt to the extreme conditions (pH 9.8 salinity 0.2% and alkalinity 151.2 meq/L) of Lake Van. In this study, it was aimed to determine the cytotoxic and genotoxic effects of chlorpyrifos (CPF) on Lake Van fish primary gill cell culture. Gill epithelium from Lake Van fish was isolated enzymatically and grown in primary culture on Leibovitz’s L-15 medium. After different doses (0.01, 0.1, 1, and 10 μM) of CPF were applied to the gill cells, the total oxidant status (TOS), total antioxidant status (TAS), malondialdehyde (MDA), and DNA damage levels (8-hydroxyguanine (8-OHdG)) were examined at the end of 24 and 48 h. It was determined that the TOS, MDA, and 8-OHdG levels increased in the cells exposed to high doses (1 and 10 μM) of CPF and the TAS was decreased (P < 0.05). It was revealed from this study that CPF administered at a dose higher than 1 μM can cause oxidative stress and DNA damage in the primary gill cell culture of Lake Van fish. In addition, the findings showed that Lake Van fish primary gill cell culture was useful in determining the effects of toxic substances likely to be the contaminants of a lake.

Download Full-text

Determination of toxic effects of deltamethrin on the primary gill cell culture of Lake Van fish [Alburnus tarichi (Güldenstadt 1814)]

Commagene Journal of Biology ◽

10.31594/commagene.1007389 ◽

2021 ◽

pp. 156-160

Author(s):

Elif KAVAL OĞUZ

Keyword(s):

Cell Culture ◽

Toxic Effects ◽

Lake Van ◽

Gill Cell

Download Full-text

Effect of Water pH on the Uptake of Acidic (Ibuprofen) and Basic (Propranolol) Drugs in a Fish Gill Cell Culture Model

Environmental Science & Technology ◽

10.1021/acs.est.0c06803 ◽

2021 ◽

Author(s):

Elisabeth Dohmann Chang ◽

Raewyn M. Town ◽

Stewart F. Owen ◽

Christer Hogstrand ◽

Nic R. Bury

Keyword(s):

Cell Culture ◽

Cell Culture Model ◽

Fish Gill ◽

Gill Cell ◽

Effect Of Water ◽

Culture Model ◽

Water Ph

Download Full-text

Environmental monitoring of urban streams using a primary fish gill cell culture system (FIGCS)

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2015.06.012 ◽

2015 ◽

Vol 120 ◽

pp. 279-285 ◽

Cited By ~ 8

Author(s):

Sabine Schnell ◽

Kafilat Bawa-Allah ◽

Adebayo Otitoloju ◽

Christer Hogstrand ◽

Thomas H. Miller ◽

...

Keyword(s):

Cell Culture ◽

Environmental Monitoring ◽

Culture System ◽

Urban Streams ◽

Cell Culture System ◽

Fish Gill ◽

Gill Cell

Download Full-text

Genotoxic Effects of Nonylphenol and Bisphenol A Exposure in Aquatic Biomonitoring Species: Freshwater Crustacean, Daphnia magna, and Aquatic Midge, Chironomus riparius

Bulletin of Environmental Contamination and Toxicology ◽

10.1007/s00128-009-9745-1 ◽

2009 ◽

Vol 83 (4) ◽

pp. 463-468 ◽

Cited By ~ 50

Author(s):

Sun-Young Park ◽

Jinhee Choi

Keyword(s):

Bisphenol A ◽

Daphnia Magna ◽

Chironomus Riparius ◽

Genotoxic Effects ◽

Freshwater Crustacean

Download Full-text

Enhanced degradation of micropollutants by zero-valent aluminum activated persulfate: assessment of toxicity and genotoxic activity

Water Science & Technology ◽

10.2166/wst.2017.489 ◽

2017 ◽

Vol 76 (12) ◽

pp. 3195-3204 ◽

Cited By ~ 2

Author(s):

T. Olmez-Hanci ◽

I. Arslan-Alaton ◽

M. Doğan ◽

S. Khoei ◽

H. Fakhri ◽

...

Keyword(s):

Surface Water ◽

Bisphenol A ◽

Vibrio Fischeri ◽

Degradation Products ◽

Distilled Water ◽

Genotoxic Effects ◽

Removal Efficiencies ◽

Relative Inhibition ◽

Activated Persulfate ◽

Enhanced Degradation

Abstract Advanced oxidation of the aqueous Triton™ X-45 (TX-45), iopamidol (IOPA), ciprofloxacin (CIP) and bisphenol A (BPA) solutions via activation of persulfate (PS) with zero-valent aluminum (ZVA) was investigated. The study aimed at assessing the effectiveness of the PS/ZVA process in terms of target micropollutants (MPs) and toxicity abatements in raw surface water (RSW) and distilled water (DW). TX-45, CIP and BPA were completely degraded after 90-minute, 120-minute and 40-minute treatment, respectively, with PS/ZVA in DW, whereas 95% IOPA removal was achieved after 120-minute (MPs = 2 mg/L; ZVA = 1 g/L; PS = 0.25 mM for CIP and BPA; PS = 0.50 mM for TX-45 and IOPA; pH = 3). TX-45 (59%), IOPA (29%), CIP (73%) and BPA (46%) removal efficiencies decreased after 120-minute PS/ZVA treatment in RSW. In DW, Vibrio fischeri toxicities of original (untreated) MPs were found as: CIP (51%) > BPA (40%) > TX-45 (15%) > IOPA (1%), and as BPA (100%) > CIP (66%) > IOPA (62%) > TX-45 (35%) in RSW. Acute toxicities of MPs and their degradation products fluctuated during PS/ZVA treatment both in DW and RSW samples and resulted in different relative inhibition values after 120-minute. The original and PS/ZVA-treated TX-45, IOPA and BPA in DW exhibited neither cytotoxic nor genotoxic effects, whereas CIP oxidation ended up in degradation products with genotoxic effects.

Download Full-text