BMP Stimulation of Alkaline Phosphatase Activity in Pluripotent Mouse C2C12 Cells is Inhibited by Dermatopontin, One of the Most Abundant Low Molecular Weight Proteins in Demineralized Bone Matrix

2006 ◽  
Vol 47 (5) ◽  
pp. 271-277 ◽  
Author(s):  
Keyvan Behnam ◽  
Samuel S. Murray ◽  
Elsa J. Brochmann
Keyword(s):  
Molecular Weight ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
C2c12 Cells ◽  
Low Molecular Weight ◽  
Low Molecular Weight Proteins ◽  
Stimulation Of

Possible Mechanisms for the Increase in Alkaline Phosphatase Activity of Lyophilized Control Material

1972 ◽  
Vol 18 (12) ◽  
pp. 1518-1523 ◽  
Author(s):  
Alistair F Smith ◽  
Barbara A Fogg
Keyword(s):  
Molecular Weight ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Gel Filtration ◽  
Low Molecular Weight ◽  
Temperature Dependent ◽  
Control Material ◽  
Rate Of Increase ◽  
Control Procedures

Abstract The increased alkaline phosphatase (EC 3.1.3.1) activity of reconstituted lyophilized control sera has recently been the focus of considerable interest because of its possible implications for quality-control procedures. We confirm that these reconstituted materials show a temperature-dependent increase in alkaline phosphatase activity, but could show no alteration in activity of fresh sera. The rate of increase in activity was unaffected by dialysis of the reconstituted material, and occurred more rapidly in dilute solutions. Studies with acrylamide gel electrophoresis and Sephadex G-200 gel filtration showed that, immediately after reconstitution, a high-molecular-weight alkaline phosphatase component predominated; during subsequent spontaneous activation this component decreased, and there was a concomitant increase in a low-molecular-weight alkaline phosphatase component. The results obtained when the reconstituted material was extracted with butanol suggest that the observed changes in alkaline phosphatase activity may be attributed to the breakdown of a complex between alkaline phosphatase and lipoprotein.

Effect of a Pulsing Electromagnetic Field on Demineralized Bone-matrix-induced Bone Formation in a Bony Defect in the Premaxilla of Rats

1992 ◽  
Vol 71 (12) ◽  
pp. 1920-1925 ◽  
Author(s):  
T. Takano-Yamamoto ◽  
M. Kawakami ◽  
M. Sakuda
Keyword(s):  
Alkaline Phosphatase ◽  
Electromagnetic Field ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Bony Defect ◽  
Defect Sites ◽  
Male Wistar Rats ◽  
Demineralized Bone

A 2-mm non-healing bony defect was prepared in the premaxilla of male Wistar rats weighing about 180 g as a simulation of an alveolar cleft, for determination of whether a pulsing electromagnetic field (PEMF) could promote regeneration of bone induced by demineralized bone matrix (DBM). The defect was either treated with 7 mg DBM or was left as a non-grafted control. The rats were exposed to a PEMF with a frequency of 100 Hz, a 10-ms-wide burst with 100 μswide quasi-rectangular pulses, repeating at 15 Hz, and magnetic field strength of 1.5-1.8 G. Alkaline phosphatase activity increased significantly from day 7 in the DBM-graft-plus-PEMF group and from day 10 in the DBM-graft group, reaching a maximum on day 14. A greater-than-two-fold rise in alkaline phosphatase activity and a three-fold rise in the amount of 45Ca incorporation in the DBM-graft-plus-PEMF group were attained compared with those of the DBM-graft group. The DBM-graft-plus-PEMF group produced more bone with almost complete osseous bridging in the defect sites than did the group treated with DBM only on day 35. The fmdings indicate that PEMF had an enhancing effect on the bone-inductive properties of the DBM through the stimulation of osteoblast differentiation induced by DBM.

Enhancement of vitamin D3 effect on bone metabolism in weanling rats orally administered zinc sulphate

1986 ◽  
Vol 111 (2) ◽  
pp. 285-288 ◽  
Author(s):  
Masayoshi Yamaguchi ◽  
Teruyuki Sakashita
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Bone Metabolism ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Dna Content ◽  
Zinc Sulphate ◽  
Simultaneous Administration ◽  
Weanling Rats ◽  
Stimulation Of

Abstract. The interaction of vitamin D3 and zinc on bone metabolism was investigated in the femur of weanling rats. Oral administration of vitamin D3 (1.0 μg/100 g body weight) did not cause any increase in the zinc accumulation in the femoral tissue following treatment with zinc sulphate (1.0 mg Zn/100 g). Administration of vitamin D3 or zinc produced significant increases the alkaline phosphatase activity and DNA content of the femoral diaphvsis but not of the epiphysis. The increase in alkaline phosphatase activity was enhanced additionally by simultaneous administration of vitamin D3 and zinc. Moreover, the increase in DNA content was enhanced markedly (about 4 times) by these treatments. At a dose of 0.5 μg of vitamin D3 per 100 g, DNA content was at the control level. This level was increased about 2 times by simultaneous administration of zinc (1.0 mg/100 g). The increase in alkaline phosphatase activity following simultaneous administration of vitamin D3 and zinc was significantly inhibited by treatment with cycloheximide, actinomycin D, or mitomycin C. Also, the increase in DNA content was completely inhibited by mitomycin C treatment. The present data suggest that the combination of vitamin D3 and zinc has a multiple effect on the stimulation of bone growth and mineralization in weanling rats, and that this effect is based on a stimulation of the DNA synthesis in bone cells.

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