Recovery Improvement of Fine Magnetic Particles by Floc Magnetic Separation

2012 ◽  
Vol 33 (3) ◽  
pp. 170-179 ◽  
Author(s):  
Subrata Roy
Keyword(s):  
Magnetic Separation ◽  
Magnetic Particles

scholarly journals Direct magnetic separation of immune cells from whole blood using bacterial magnetic particles displaying protein G

2009 ◽  
Vol 25 (1) ◽  
pp. 219-226 ◽  
Author(s):  
Masayuki Takahashi ◽  
Tomoko Yoshino ◽  
Haruko Takeyama ◽  
Tadashi Matsunaga
Keyword(s):  
Magnetic Separation ◽  
Immune Cells ◽  
Whole Blood ◽  
Magnetic Particles ◽  
Protein G ◽  
Bacterial Magnetic Particles

scholarly journals Simulation of Magnetophoretic Separation Processes in Dispersions of Superparamagnetic Nanoparticles in the Noncooperative Regime

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Jordi S. Andreu ◽  
Pablo Barbero ◽  
Juan Camacho ◽  
Jordi Faraudo
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetic Separation ◽  
Magnetic Particles ◽  
Colloidal Particles ◽  
Low Cost ◽  
Superparamagnetic Nanoparticles ◽  
Separation Processes ◽  
Magnetic Separator ◽  
Simulation Strategy ◽  
Magnetophoretic Separation

Magnetic separation has gained much attention due to its implications in different fields, becoming feasible as an alternative to existent technologies at the industrial and lab scale. Substantial efforts are focused to improve the magnetic particles used in these applications. Here we show how a relatively simple and low-cost simulation strategy (tracer simulations) can be employed to predict the effect of various key factors in magnetic separation processes, namely, particle properties and magnetic separator designs. For concreteness, we consider here specific problems in magnetic separation. The first one is the effect of different profiles of the magnetic field in the separation of magnetic nanoparticles, and the second one is the magnetophoresis of colloidal particles in a dispersion of magnetic nanoparticles.

Modification of the TUBEX typhoid test to detect antibodies directly from haemolytic serum and whole blood

2008 ◽  
Vol 57 (11) ◽  
pp. 1349-1353 ◽  
Author(s):  
Frankie C. H. Tam ◽  
Danny T. M. Leung ◽  
C. H. Ma ◽  
Pak-Leong Lim
Keyword(s):  
Typhoid Fever ◽  
Magnetic Separation ◽  
Whole Blood ◽  
Magnetic Particles ◽  
Original Method ◽  
Serum Antibodies ◽  
Assay Sensitivity ◽  
Simple Modification ◽  
Colour Changes ◽  
Larger Sample

The TUBEX test for typhoid fever detects serum antibodies in a simple and rapid assay system based on the inhibition of binding between two types of reagent particles — magnetic particles coated with an antigen (Salmonella O9 LPS) and coloured indicator particles coated with an anti-O9 mAb. A magnet is used to separate the colour indicator particles bound to the magnetic particles from the unbound indicator particles. Specific colour changes following magnetic separation are indicative of antibodies in the patient's serum; however, because results are interpreted based on changes in the colour red, haemolytic or icteric specimens cannot be used. This study describes a simple modification of the protocol to accommodate such specimens, including whole blood. This involves the addition of a quick and simple washing step after mixing the specimen with the antigen-bound magnetic particles. This modification has the advantage of allowing larger sample volumes to be used, thus enhancing the assay sensitivity, and also enables cases considered to be borderline positive by the original method to be re-assessed.

scholarly journals Rapid detection and purification of sequence specific DNA binding proteins using magnetic separation

2006 ◽  
Vol 71 (2) ◽  
pp. 135-141 ◽  
Author(s):  
Marija Mojsin ◽  
Jelena Djurovic ◽  
Isidora Petrovic ◽  
Aleksandar Krstic ◽  
Danijela Drakulic ◽  
...  
Keyword(s):  
Dna Binding ◽  
Magnetic Separation ◽  
Magnetic Particles ◽  
Binding Proteins ◽  
Rapid Identification ◽  
Dna Binding Proteins ◽  
Binding Activity ◽  
High Yield ◽  
Dna Binding Activity ◽  
Putative Binding Site

In this paper, a method for the rapid identification and purification of sequence specific DNA binding proteins based on magnetic separation is presented. This method was applied to confirm the binding of the human recombinant USF1 protein to its putative binding site (E-box) within the human SOX3 protomer. It has been shown that biotinylated DNA attached to streptavidin magnetic particles specifically binds the USF1 protein in the presence of competitor DNA. It has also been demonstrated that the protein could be successfully eluted from the beads, in high yield and with restored DNA binding activity. The advantage of these procedures is that they could be applied for the identification and purification of any high-affinity sequence-specific DNA binding protein with only minor modifications.

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