Transaminase activity rhythms in Penicillium claviforme

<p>Teratogenic effect of herbicide glyphosate-Roundup, sold under the name Glifotox on Wistar rats was evaluated. The biological material was treated intraperitoneally with glyphosate at concentrations of 100, 125, and 150 mg/kg from gestation day nine. Hysterectomy was performed on day 18 of gestation, and the uterine horns where the embryos were located, in addition to recording the percentage of malformed embryos by modifying the method of Wilson were observed. The liver was removed and quantified by spectrophotometry with transaminase activity showed higher concentrations malformation rate and higher enzyme activity was 125 mg/kg, below is the average of 100 mg/kg and higher concentrations such as 150 mg/kg a large number of resorptions was obtained. It is concluded that glyphosate is toxic affecting the liver and liver enzymes involved in the formation of amino acids also produce delay in embryonic development.</p>

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The effect of acceptor modification upon the synthesis of dinucleoside phosphates catalyzed by non-specific ribonucleases of Penicillium claviforme

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19790613 ◽

1979 ◽

Vol 44 (2) ◽

pp. 613-625 ◽

Cited By ~ 2

Author(s):

Valentina I. Gulyaeva ◽

Antonín Holý

Keyword(s):

Reaction Center ◽

Hydroxy Group ◽

Pyrimidine Ring ◽

Phosphate Group ◽

Mutual Orientation ◽

Acceptor Molecule ◽

Heterocyclic Base ◽

Sugar Moiety ◽

Cyclic Phosphate ◽

Penicillium Claviforme

The present paper studies the effect of the modification of heterocyclic base, sugar moiety and the presence of phosphate group on the nucleoside acceptors in the synthesis of dinucleoside phosphates from adenosine 2',3'-cyclic phosphate as donor, catalyzed by nonspecific acidic extracellular and intracellular ribonucleases from Penicillium claviforme. The enzyme binds specifically the acceptor molecule, preferring cytosine nucleosides. It requires the presence of the whole sugar moiety, an exact mutual orientation of the heterocyclic base and the reaction center (5'-hydroxy group), and a suitable conformation of the acceptor molecule. The enzyme-acceptor bond is homochiral and the presence of the N3-H group in the pyrimidine ring is important. The reaction between the donor and the acceptor is bimolecular and is competitively inhibited by some purine nucleosides.

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25-Hydroxycholecalciferol Concentration Is Associated with Protein Loss and Serum Albumin Level during the Acute Phase of Burn Injury

Nutrients ◽

10.3390/nu12092780 ◽

2020 ◽

Vol 12 (9) ◽

pp. 2780

Author(s):

Andrzej Krajewski ◽

Krzysztof Piorun ◽

Dominika Maciejewska-Markiewicz ◽

Marta Markowska ◽

Karolina Skonieczna-Żydecka ◽

...

Keyword(s):

Vitamin D ◽

Protein Concentration ◽

Serum Albumin Level ◽

Burn Injury ◽

Albumin Level ◽

Albumin Concentration ◽

Transaminase Activity ◽

C Reactive Protein ◽

Reactive Protein ◽

25 Hydroxycholecalciferol

Background: Burned patients have an increased need for vitamin D supply related to the maintenance of calcium–phosphate homeostasis and the regulation of cell proliferation/differentiation. This study aimed to analyze the concentration of 25-hydroxycholecalciferol and its relationship with severe condition after burn injury. Methods: 126 patients were enrolled in the study. Patients were qualified due to thermal burns—over 10% of total body surface area. On the day of admission, the following parameters were assessed: 25-hydroxycholecalciferol concentration, total protein concentration, albumin concentration, aspartate transaminase activity, alanine transaminase activity, albumin concentration, creatinine concentration, c-reactive protein concentration, procalcitonin concentration, and interleukin-6 concentration. Results: Almost all patients (92%) in the study group had an improper level of vitamin D (<30 ng/mL), with the average of 11.6 ± 10.7 ng/mL; 17.5% of patients had levels of vitamin D below the limit of determination—under 3 ng/mL. The study showed that there are several factors which correlated with vitamin D concentration during the acute phase of burn injury, including: total protein (r = 0.42, p < 0.01), albumin, (r = 0.62, p < 0.01), percentage of body burns (r = 0.36, p < 0.05), aspartate aminotransferase (r = 0.21, p < 0.05), and c-reactive protein (r = 0.22, p < 0.05). We did not find any significant correlation between vitamin D concentration and body mass index. Conclusions: The burn injury has an enormous impact on the metabolism and the risk factors of the deficiency for the general population (BMI) have an effect on burned patients. Our study showed that concentration of 25-hydroxycholecalciferol is strongly correlated with serum albumin level, even more than total burn surface area and burn degrees as expected. We suspect that increased supplementation of vitamin D should be based on albumin level and last until albumin levels are balanced.

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0897 Low Stability In Rest Activity Rhythms Is Associated With Higher Suicidal Ideation In Adolescents With Bipolar Disorder

SLEEP ◽

10.1093/sleep/zsz067.895 ◽

2019 ◽

Vol 42 (Supplement_1) ◽

pp. A360-A361

Author(s):

Qianyi Chen ◽

Peter Franzen ◽

Tina Goldstein ◽

Sarah Gratzmiller

Keyword(s):

Bipolar Disorder ◽

Suicidal Ideation ◽

Activity Rhythms ◽

Rest Activity

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A Systematic Review of Evidence for a Role of Rest-Activity Rhythms in Dementia

Frontiers in Psychiatry ◽

10.3389/fpsyt.2019.00778 ◽

2019 ◽

Vol 10 ◽

Cited By ~ 4

Author(s):

Stephen F. Smagula ◽

Swathi Gujral ◽

Chandler S. Capps ◽

Robert T. Krafty

Keyword(s):

Systematic Review ◽

Activity Rhythms ◽

Rest Activity

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753 The role of circadian activity rhythms in depressive symptoms among female nursing students

SLEEP ◽

10.1093/sleep/zsab072.750 ◽

2021 ◽

Vol 44 (Supplement_2) ◽

pp. A293-A294

Author(s):

Xin Zhang ◽

Shih-Yu Lee

Keyword(s):

Nursing Education ◽

Depressive Symptoms ◽

Nursing Students ◽

Conceptual Model ◽

Depression Scale ◽

Psychological Problems ◽

Circadian Activity ◽

Activity Rhythms ◽

Wrist Actigraphy ◽

Insomnia Symptoms

Abstract Introduction Depression is prevalent among nursing students. Rumination and sleep-wake rhythms are associated to mental illness; however, no clear path has been found. This exploratory study aimed to examine the associations among circadian activity rhythms (CAR), rumination, and depressive symptoms in female nursing students; further, to test a hypothesized CAR conceptual model. Methods A total of 148 female nursing junior students in China completed a battery of questionnaires, including Athens Insomnia Scale (AIS), Ruminative Responses Scale (RRS), and Self-rating Depression Scale (SDS). Wrist actigraphy was used to collect total sleep time, CAR, and acrophase (time of the peak of the fitted activity curve). The path analysis was explored by using SPSS and AMOS. Results The mean age of the students was 20.64 years (SD = 0.86). About 58.8% of the participants were either mild or moderate depressed. About 93.9% of the students reported significant insomnia symptoms (AIS scores >6). Rumination was measured by the RRS (M= 2.01, SD = 0.54), and students scored higher in brooding than that of reflective pondering (2.07 vs. 1.95). The average of TST was 394.59 minutes (SD = 51.92). The CAR ranged from 0.40 to 0.98, with a mean of 0.75 (SD = 0.11). The acrophase ranged from 12:46 to 20:14 (median 16:30), with a later acrophase indicates of a more delayed circadian phase. The final model shows satisfactory fit (χ2= 2.238, p= .327); a better CAR can indirectly reduce depressive symptoms by directly reducing brooding (B = -1.149) and improving insomnia symptoms (B = -6.6443). Conclusion In order to prevent psychological problems of nursing students, ruminating and CAR should be part of health screening. The novel conceptual model provides a basis for reforming nursing education to prevent psychological problems. Support (if any) Chinese National Natural Science Foundation [71603279]

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