Assessment of endometrial receptivity during implantation window in women with unexplained infertility

2020 ◽  
Vol 36 (10) ◽  
pp. 917-921
Author(s):  
Li Wang ◽  
Shulan Lv ◽  
Wenjun Mao ◽  
Meili Pei ◽  
Xiaofeng Yang
Keyword(s):  
Endometrial Receptivity ◽  
Unexplained Infertility ◽  
Implantation Window

scholarly journals Cellular homeostasis, implantation window and unexplained infertility: role of phosphatase and tensin homolog deleted on chromosome 10

2019 ◽  
Vol 8 (7) ◽  
pp. 2754
Author(s):  
Annu Makker ◽  
Madhu Mati Goel ◽  
Kumari Manu ◽  
Renu Makker
Keyword(s):  
Cell Proliferation ◽  
Unexplained Infertility ◽  
Endometrial Cell ◽  
Chromosome 10 ◽  
Cellular Homeostasis ◽  
Phosphatase And Tensin Homolog ◽  
Implantation Window ◽  
Tensin Homolog ◽  
Proliferation And Apoptosis

Background: Balance between endometrial cell proliferation and apoptosis is crucial for successful embryo implantation. PTEN (phosphatase and tensin homolog deleted on chromosome 10), a pro-apoptotic factor, is proposed to be one of the signaling proteins through which estrogen and progesterone act to affect cellular homeostasis. Although reports in literature have suggested role of PTEN in regulating endometrial cell proliferation and apoptosis during window of implantation, its involvement in women with unexplained infertility is not clear. In the present study, we examined expression, cellular distribution and activation status of PTEN, cell proliferation, and apoptosis in midsecretory endometrium from women with unexplained infertility as compared to fertile controls.Methods: Endometrial biopsies from infertile (n=11) and fertile women (n=22) were used for immunohistochemical evaluation of PTEN, phospho-PTEN and Ki67. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay was performed for detection of apoptotic cells.Results: Biopsies from infertile women as compared to fertile controls demonstrated statistically significant: i) decrease in nuclear PTEN (P < 0.001), increase in nuclear phospho-PTEN (P < 0.05), increase in nuclear and cytoplasmic phospho-PTEN/PTEN ratio (P < 0.001 and P < 0.05 respectively) in endometrial stroma, ii) increase in cytoplasmic phospho-PTEN (P < 0.001) and phospho-PTEN/PTEN ratio (P < 0.05) in glandular epithelium (GE), iii) increase in Ki67 labeling in GE (P < 0.01) and stroma (P < 0.05) and, iv) decrease in (P < 0.001) apoptosis.Conclusions: Altered PTEN expression and associated modulation in cellular homeostasis during the implantation window might contribute to mechanism underlying unexplained infertility.

scholarly journals Letrozole Versus Clomiphene Citrate and Natural Cycle: Endometrial Receptivity During Implantation Window in Women With Polycystic Ovary Syndrome

2021 ◽  
Vol 11 ◽  
Author(s):  
Li Wang ◽  
Shulan Lv ◽  
Fen Li ◽  
E. Bai ◽  
Xiaofeng Yang
Keyword(s):  
Predictive Value ◽  
Integrin Αvβ3 ◽  
Endometrial Receptivity ◽  
Pregnancy Rates ◽  
Flow Index ◽  
Natural Cycle ◽  
Ongoing Pregnancy ◽  
Implantation Window ◽  
Uterine Fluid ◽  
Ultrasonic Parameters

ObjectiveNumerous studies have reported on ovulation and pregnancy rates in patients with polycystic ovary syndrome (PCOS). However, relevant data on endometrial receptivity are limited. This study was conducted to compare endometrial receptivity during implantation windows among letrozole (LE), clomiphene citrate (CC), and natural cycle, and to assess the predictive value for pregnancy of observed indicators.MethodsThis randomized controlled trial study enrolled 270 patients with PCOS. Patients were given LE (n=90) at a dose of 2.5mg/day or CC (n=90) at a dose of 50 mg/day on cycle days 5–9 for ovulation induction. Patients in the natural cycle group (n=90) did not receive any drug for ovulation induction. Endometrial ultrasonic parameters, integrin αvβ3, and vascular endothelial growth factor (VEGF) concentrations in uterine secretion were detected during the implantation window. The endometrial receptivity, ovulation rate, pregnancy rates, and predictive value of observed indicators for pregnancy were analyzed.ResultsThe successful ovulation rate did not differ between the LE group and CC group (P&gt;0.05). Endometrial ultrasonic parameters [endometrial thickness (ET), endometrial volume (EV), vascularization index (VI), flow index (FI), vascularization flow index (VFI)], integrin αvβ3, and VEGF concentrations in uterine fluid were significantly higher in the LE group compared with the CC group and natural cycle group (P&lt;0.05). The clinical pregnancy and ongoing pregnancy rates of the LE group were significantly higher than in the CC group (P&lt;0.05). Endometrial ultrasonic parameters (VI, FI, and VFI), integrin αvβ3, and VEGF concentrations in uterine fluid of all pregnancy groups were significantly higher compared with the no pregnancy group (P&lt;0.05), and the above parameters in ongoing pregnancy were significantly higher than in biochemical pregnancy (P&lt;0.05). The endometrial FI during the implantation window had the highest predictive value for pregnancy (AUC=0.889). The integrin αvβ3 in uterine fluid had better predictive value (AUC=0.876) than VEGF.ConclusionsEndometrial receptivity during the implantation window of LE is superior to CC in PCOS women, which may be related to higher clinical pregnancy and ongoing pregnancy rates. Endometrial FI examined by 3-D power Doppler, and integrin αvβ3 in uterine secretion during the implantation window, could be preferable non-invasive predictor markers for pregnancy.Clinical Trial Registrationwww.chictr.org.cn, ChiCTR1900023423.

scholarly journals Endometrial Receptivity and Scoring for Prediction of Implantation and Newer Markers

2010 ◽  
Vol 4 (4) ◽  
pp. 439-446 ◽  
Author(s):  
JP Rao ◽  
Narendra Malhotra ◽  
Neelam Mishra
Keyword(s):  
Color Doppler ◽  
Endometrial Receptivity ◽  
Blood Flows ◽  
Implantation Window

ABSTRACT There are many markers to indicate the preparation of endometrium for successful implantation, and these manifest during the implantation window. Today TVS color Doppler can reliably show the increase in blood flows during the peri- and postovulatory phase and objectively assess these flows to predict endometrial receptivity.

Expression of MSX1, HOXA11 and TP53I3 in the endometrium associated with the onset of pregnancy after repeated failed IVF attempts in patients with tubo-peritoneal factor infertility

GYNECOLOGY ◽  
2020 ◽  
Vol 22 (1) ◽  
pp. 23-28
Author(s):  
Ekaterina A. Knyazeva ◽  
Maria V. Kuznetsova ◽  
Olga V. Burmenskaya ◽  
Andrey E. Donnikov ◽  
Elena A. Kalinina
Keyword(s):  
Gene Expression ◽  
Transcriptome Profiling ◽  
Endometrial Receptivity ◽  
In Vitro Fertilisation ◽  
Ivf Outcome ◽  
Program Outcomes ◽  
Implantation Window ◽  
Genome Wide ◽  
A Genome

Relevance. The success of the in vitro fertilisation (IVF) program, among other factors, depends on the readiness of the endometrium to accept the embryo. It is believed that this is possible during the so-called implantation window, the timing of which can be shifted under the influence of various factors. Evaluation of endometrial receptivity and the implantation window based on analysis of endometrial gene expression before embryo transfer is a promising approach for predicting the likelihood of pregnancy in IVF programs. Aim. To construct a classifier based on the expression of endometrial genes for predicting the outcome of an IVF program in patients with tubal-peritoneal infertility factor and repeated failed IVF attempts in history. Materials and methods. Before the IVF program, a genome-wide transcriptome profiling of endometrial samples of 15 women with tubal-perioneal infertility factor and repeated unsuccessful IVF attempts in history was carried out using Affymetrix arrays. Potential genes capable of classifying IVF program outcomes were selected, after which the expression of these genes was analyzed by qPCR-RT in the endometrium of 47 women to construct IVF outcome classifiers based on the expression of pairs or triples of genes. Results. A classifier based on the expression of the triple of genes MSX1 (HOX7), HOXA11, and TP53I3 made it possible to determine the onset of pregnancy in an IVF program with a sensitivity of 73% and a specificity of 71% with an area under the ROC-curve (AUC) of 0.738 (95% confidence interval 0.5770.898). Earlier, a relationship was found between the expression of these genes and receptivity of the endometrium, which suggests that these genes play a role in the onset of the implantation window. Conclusions. The use of a classifier based on the genes MSX1 (HOX7), HOXA11, and TP53I3 can determine the readiness of the endometrium to accept an embryo and create an individual prognosis of the outcome of an IVF program in women with tubal-peritoneal infertility factor and repeated failed IVF attempts in history.

scholarly journals GATA binding protein 2 expression at implantation window diminishes in women with adenomyosis: implications for impaired endometrial receptivity

2019 ◽  
Vol 112 (3) ◽  
pp. e330-e331
Author(s):  
Joung Woul Kim ◽  
Chih-Feng Yen ◽  
Rachel Grimes Sprague ◽  
Asli Ozmen ◽  
Nihan Semerci ◽  
...  
Keyword(s):  
Binding Protein ◽  
Endometrial Receptivity ◽  
Implantation Window

scholarly journals Impact of Kuntai Capsules on LIF, IGF-1 and EGF Expression in the Implantation Window of Endometrium in Mice

2018 ◽  
Author(s):  
Li Tan ◽  
Xiao-Xu Li ◽  
Xi-Ying Chu ◽  
Yan Li ◽  
Li-Jing Wan
Keyword(s):  
Growth Factor ◽  
Mrna Expression ◽  
Controlled Ovarian Hyperstimulation ◽  
Endometrial Receptivity ◽  
Control Group ◽  
Factor I ◽  
Implantation Window ◽  
Fluorescence Quantitative Pcr ◽  
Epidermal Growth ◽  
Pcr Method

Objective: This study aims to investigate the influence of Kuntai capsules on the expression level of leukemia inhibitory factor (LIF), insulin-like growth factor-I (IGF-1) and epidermal growth factor (EGF) during the mouse implantation window of superovulation period and controlled ovarian hyperstimulation period. Methods: A total of 90 female mice were randomly divided into six groups (n=15, each group). The RNA expression of EGF, LIF and IGF-1 in the endometrium on the 4th day of pregnancy was detected using the fluorescence quantitative PCR method, and the relative expression of these three factors in each group was compared. Results: The mRNA expression of these three factors in the endometrium during the mouse implantation window period was significantly lower in the superovulation group and COH group than in the control group (P<0.001), and was obviously lower in the superovulation group than in the COH group (P<0.05). The mRNA expression of these three factors in the endometrium remained obviously lower in the superovulation plus kuntai capsule group and COH plus kuntai capsule group, compared with the control group (P<0.01). The mRNA expression of these three factors in the endometrium was lower in control group than in the NS plus kuntai capsule group (P<0.05). Furthermore, the expression in the superovulation and COH groups was lower than in the COH plus kuntai capsule group (P<0.05). Conclusions: Both the superovulation and COH procedure could reduce the mouse endometrial receptivity. The application of the Chinese medicine Kuntai capsule could partially improve a mouse endometrial receptivity through the increasing expression of three relevant cytokines during the implantation window.

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