Ultrasonic wave propagation in thermally treated concrete up to 400 ºC

The ultrasonic pulse velocity, obtained by ultrasonic non-destructive testing, has been applied to evaluate the concrete integrity. The attenuation parameters have shown more sensitivity to damage detection in the microstructure of concrete since they consider the entire ultrasonic waveform. However, it is still necessary to evaluate the sensitivity of those parameters to thermally damaged concrete. This work aims to assess the behavior and the sensitivity of the following ultrasonic parameters: pulse and group velocities, maximum amplitude, total energy, accumulated energy, and time instants corresponding to 25%, 50%, and 75% of the energy, in detecting changes due to thermal degradation of the concrete. A sample of 39 cylindrical concrete specimens with 100 mm in diameter and 300 mm in length and C25 strength class was used. The sample was distributed into 5 groups heated between 20 and 400 ºC until the internal temperature of the specimens became homogeneous. The groups were cooled inside a muffle furnace until reaching 150 ºC. Subsequently, they were exposed to the ambient temperature and humidity of the laboratory environment for, at least, 24 hours prior to the tests of mass loss, ultrasound, and compressive strength. The results show that the ultrasonic parameters are sensitive to the thermal degradation of the concrete. The pulse velocity, the accumulated energy, and the time instants corresponding to percentages of the energy decrease monotonically as the temperature increases. The group velocity shows significant dispersions, while the maximum amplitude and the total energy increase at 200 ºC. The results led to the conclusion that the pulse velocity is the least sensitive parameter, while the time instants corresponding to 25%, 50%, and 75% of the energy are the most sensitive parameters in detecting changes due to thermal degradation of the concrete.

Elastic, Mechanical and Thermophysical properties of Single-Phase Quaternary ScTiZrHf High-Entropy Alloy

Consequent to the interaction potential model, the high-order elastic constants at high entropy alloys in single-phase quaternary ScTiZrHf have been calculated at different temperatures. Elastic constants of second order (SOECs) helps to determine other ultrasonic parameters. With the help of SOECs other elastic moduli, bulk modulus, shear modulus, Young’s modulus, Pugh’s ratio, elastic stiffness constants and Poisson’s ratio are estimated at room temperature for elastic and mechanical characterization. The other ultrasonic parameters are calculated at room temperature for elastic and mechanical characterization. The temperature variation of ultrasonic velocities along the crystal's z-axis is evaluated using SOECs. The temperature variation of the average debye velocity and the thermal relaxation time (τ) are also estimated along this orientation axis. The ultrasonic properties correlated with elastic, thermal and mechanical properties which is temperature dependent is also discussed. The ultrasonic attenuation due to phonon – phonon (p-p) interactions is also calculated at different temperatures. In the study of ultrasonic attenuation such as a function of temperature, thermal conductivity appears to be main contributor and p- p interactions are the responsible reason of attenuation and found that the mechanical properties of the high entropy alloy ScTiZrHf are superior at room temperature.

Ultrasound-Assisted Production of Xylo-Oligosaccharides From Alkali-Solubilized Corncob Bran Using Penicillium janthinellum XAF01 Acidic Xylanase

A novel treatment involving enzymatic hydrolysis using an acidic xylanase coupled with ultrasound was performed to improve the xylo-oligosaccharides (XOS) yield from corncob bran. The acidic xylanase (XynB) was purified to a most suitable pH, temperature, and operational parameters for ultrasound-assisted hydrolysis were determined. A preliminary mechanistic investigation was performed through circular dichroism (CD) spectroscopy, scanning electron microscope (SEM) and a laser particle size analyzer, and the effects of ultrasound on enzyme (XynB) and substrate (corncob bran) were assessed. The results show that the maximum XOS yield was 20.71% when the reaction pH and temperature were 4.3 and 50°C, the ultrasonic parameters were 50 kHz and 0.40 W/cm2, which was 2.55 fold higher than that obtained using a non-ultrasound-assisted enzymatic preparation. Mechanism studies indicated that ultrasonic pretreatment could reduce the β-fold content and increase the random coil content. Changes in structure and size of substrate were observed. The specific surface area of the XAC molecules is easy to carry out enzymatic reaction, which is beneficial to the production of XOS.

A Setup for Microscopic Studies of Ultrasounds Effects on Microliters Scale Samples: Analytical, Numerical and Experimental Characterization

Sonoporation is the process of cell membrane permeabilization, due to exposure to ultrasounds. There is a lack of consensus concerning the mechanisms of sonoporation: Understanding the mechanisms of sonoporation refines the choice of the ultrasonic parameters to be applied on the cells. Cells’ classical exposure systems to ultrasounds have several drawbacks, like the immersion of the cells in large volumes of liquid, the nonhomogeneous acoustic pressure in the large sample, and thus, the necessity for magnetic stirring to somehow homogenize the exposure of the cells. This article reports the development and characterization of a novel system allowing the exposure to ultrasounds of very small volumes and their observation under the microscope. The observation under a microscope imposes the exposure of cells and Giant Unilamellar Vesicles under an oblique incidence, as well as the very unusual presence of rigid walls limiting the sonicated volume. The advantages of this new setup are not only the use of a very small volume of cells culture medium/microbubbles (MB), but the presence of flat walls near the sonicated region that results in a more homogeneous ultrasonic pressure field, and thus, the control of the focal distance and the real exposure time. The setup presented here comprises the ability to survey the geometrical and dynamical aspects of the exposure of cells and MB to ultrasounds, if an ultrafast camera is used. Indeed, the setup thus fulfills all the requirements to apply ultrasounds conveniently, for accurate mechanistic experiments under an inverted fluorescence microscope, and it could have interesting applications in photoacoustic research.