Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates

Pelargonium graveolens (Geranium) is a source of the finest quality of fragrance and its essential oils are used as antibacterial, and antifungal agents. The aim of the current research is to determine chemical constituents in the essential oil of P. graveolens by GC-MS and evaluate its antidiabetic activity via α-glucosidase inhibition assay. The chemical composition of P. graveolens essential oil was determined by GC/MS and its antidiabetic activity was assessed through inhibition of α-glucosidase enzyme in in-vitro models. GC-MS analysis determines 36 chemical components in the essential oil of P. graveolens leaves, and citronellyl isovalerate (10.41 %), menthol (9.61 %), linalool (8.63 %), p-menthone (6.31 %), and geranyl tiglate (4.99 %) were recorded as major constituents. The essential oil of P. graveolens leaves showed concentration dependant inhibition of α-glucosidase enzyme ranging from 28.13±1.41 to 74.24±2.53 µg/mL for concentration ranging from 31.25 to 1000 µg/mL. The IC50 values for of P. graveolens and acarbose were found as 93.72±4.76 and 80.4±2.17 µg/mL, respectively against the α-glucosidase enzyme. The study finding explores the chemical components of P. graveolens growing in the Iraqi Kurdistan region and scientifically supported its possible use in diabetic patients for controlling postprandial hyperglycemia.

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Modulatory effect of Syzygium aromaticum and Pelargonium graveolens on oxidative stress and inflammation

10.1101/362426 ◽

2018 ◽

Author(s):

Ilias Marmouzi ◽

El Mostafa Karym ◽

Rachid Alami ◽

Meryem El Jemli ◽

Mourad Kharbach ◽

...

Keyword(s):

Oxidative Stress ◽

Essential Oils ◽

Toxic Effect ◽

Tetrahymena Pyriformis ◽

Syzygium Aromaticum ◽

Minimal Risk ◽

Pelargonium Graveolens ◽

Anti Inflammatory

AbstractBackgroundTherapy combination is defined as disease treatment with two or more medication to acheive efficacy with lower doses or lower toxicity. Regarding its reported toxicities and efficacy, the Essential Oils (EOs) from Syzygium aromaticum (SA) and Pelargonium graveolens (PG) were combined for in vitro and in vivo assays and toxicities.MethodsThe Essential Oils and mixture were tested for in vivo/in vitro antioxidant and anti-inflammatory activities. The assays included the animal model of acute inflammation (carrageenan model), the protective effect on H2O2/Sodium nitroprissude induced stress in Tetrahymena pyriformis, and the in vitro antioxidant assays.ResultsThe chemical analysis of the investigated Oils has lead to the identification of Eugenol (74.06%), Caryophyllene (11.52%) and Carvacrol acetate (7.82%) as the major element in SA; while PG was much higher in Citronellol (30.77%), 10-epi-γ-Eudesmol (22.59%), and Geraniol (13.95%). In our pharmacological screening of samples, both Oils demonstrated good antioxidant effects. In vivo investigation of the antioxidant activity in the protozoa model (T. pyriformis) demonstrated a lesser toxic effect of EOs mixture with no significant differences when oxidative stress markers and antioxidant enzymes (MDA, SOD and CAT) were evaluated. On the other hand the in vivo model of inflammatory response to carrageenan demonstrated a good inhibitory potential of both EOs. The EOs Mixture demonstrated equivalent bioactivity with lower toxic effect and minimal risk for each compound.ConclusionsThe results from this study indicate that EOs mixture from SA and PG demonstrated promising modulatory antioxidant/anti-inflammatory effect, which suggest an efficient association for therapy.

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Antifungal Activity of Plant's Essential Oils against Post Harvest Fungal Disease of Apple Fruit

Forestry: Journal of Institute of Forestry, Nepal ◽

10.3126/forestry.v16i0.28361 ◽

2019 ◽

Vol 16 ◽

pp. 86-100

Author(s):

Nirjan Oli ◽

Uday Kumar Singh ◽

Sanjay Kumar Jha

Keyword(s):

Antifungal Activity ◽

Essential Oils ◽

Apple Fruit ◽

Pathogenicity Test ◽

Eucalyptus Citriodora ◽

Mycelium Growth ◽

Cinnamomum Tamala ◽

Postharvest Rot ◽

Apple Fruits

Bioactive natural compounds are developed as alternatives to synthetic fungicides for the control of rot diseases of apple fruit. The antifungal activity of essential oils exudes from five plants, namely, Cinnamomum tamala, Lantana camara, Ageratina adenophora, Citrus limetta and Eucalyptus citriodora were evaluated in vitro against Colletotrichum gloeosporioides, Fusarium oxysporum and Alternaria alternata causing postharvest rot disease in apple fruits. The pathogens were isolated from infected apple fruits collected from local markets of Kathamandu, Nepal. The essential oils were extracted through hydro-distillation process using Clevenger apparatus. The pathogenicity test was confirmed by inoculating pathogen into healthy apple fruit. The assessment of fungi toxicity was carried out by poison food technique using five different concentrations: 2.5μl/ml, 5μl/ml, 10μl/ml, 20μl/ml and 40μl/ml and controls were set to determine percentage inhibition of mycelial growth to test fungi. Among tested five essential oils, Cinnamomum tamala showed most effective antifungal activity against all three pathogens, which inhibited mycelium growth by 100% at 40 μl/ml concentrations. However, Eucalyptus citriodora showed all three pathogens inhibited mycelium growth by 65.87%, 73.17% and 86.91%, respectively at 40 μl/ml concentration.

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Antifungal mechanism of the combination of Cinnamomum verum and Pelargonium graveolens essential oils with fluconazole against pathogenic Candida strains

Applied Microbiology and Biotechnology ◽

10.1007/s00253-017-8442-y ◽

2017 ◽

Vol 101 (18) ◽

pp. 6993-7006 ◽

Cited By ~ 20

Author(s):

Rym Essid ◽

Majdi Hammami ◽

Dorra Gharbi ◽

Ines Karkouch ◽

Thouraya Ben Hamouda ◽

...

Keyword(s):

Essential Oils ◽

Pelargonium Graveolens ◽

Antifungal Mechanism

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The Chemical Composition and Anti-mycobacterial Activities of Trachyspermum copticum and Pelargonium graveolens Essential Oils

Recent Patents on Anti-Infective Drug Discovery ◽

10.2174/1574891x14666191028113321 ◽

2020 ◽

Vol 15 (1) ◽

pp. 68-74 ◽

Cited By ~ 1

Author(s):

Jalil Kardan-Yamchi ◽

Mohaddese Mahboubi ◽

Hossein Kazemian ◽

Gholamreza Hamzelou ◽

Mohammad M. Feizabadi

Keyword(s):

Mycobacterium Tuberculosis ◽

Chemical Composition ◽

Minimum Inhibitory Concentration ◽

Essential Oil ◽

Essential Oils ◽

Inhibitory Concentration ◽

Mycobacterium Fortuitum ◽

Drug Resistant ◽

Pelargonium Graveolens ◽

Trachyspermum Copticum

Background: Microbial resistance to antibiotics and their adverse effects related to these antibiotics are a matter of global public health in the 21th century. The emergence of drug-resistant strains, has gained the interest of the scientists to discover new antimicrobial agents from the essential oil of medicinal plants. Methods: Anti-mycobacterial effects of Trachyspermum copticum and Pelargonium graveolens essential oils were determined against multi-drug resistant clinical strains of Mycobacterium tuberculosis, Mycobacterium kansasii, Mycobacterium fortuitum and standard strain of Mycobacterium tuberculosis H37Rv by a Broth micro-dilution method. Pelargonium graveolens plant named Narmada was discovered by Kulkarni R.N et al. (Patent ID, USPP12425P2) and a formulation comprising thymol obtained from Trachyspermum is useful in the treatment of drug-resistant bacterial infections (Patent ID, US6824795B2). The chemical composition of hydro-distilled essential oils was determined by GC and GC-MS. Results: Minimum Inhibitory Concentration (MIC) values for T. copticum essential oil against tested isolates were ranged from 19.5 µg/mL to 78 µg/mL. The least minimum inhibitory concentration of P. graveolens extract against M. Kansasii and MDR-TB was 78 µg/ml. Conclusion: The results of the present research introduced T. copticum and P. graveolens essential oils as a remarkable natural anti-mycobacterial agent, but more pharmacological studies are required to evaluate their efficacy in animal models.

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Use of Dimethyldioxirane in the Epoxidation of the Main Constituents of the Essential Oils Obtained from Tagetes Lucida, Cymbopogon Citratus, Lippia Alba and Eucalyptus citriodora

Natural Product Communications ◽

10.1177/1934578x1100600701 ◽

2011 ◽

Vol 6 (7) ◽

pp. 1934578X1100600 ◽

Cited By ~ 1

Author(s):

Luz A. Veloza ◽

Lina M. Orozco ◽

Juan C. Sepúlveda-Arias

Keyword(s):

Essential Oils ◽

Organic Synthesis ◽

Oxygen Transfer ◽

Environmentally Friendly ◽

Acetone Solution ◽

Oxidation Reactions ◽

Eucalyptus Citriodora ◽

Cymbopogon Citratus ◽

Lippia Alba

Dimethyldioxirane (DMDO), a widely used oxidant in organic synthesis is considered an environmentally friendly oxygen transfer reagent because acetone is the only byproduct formed in its oxidation reactions. This work describes the isolation of the main constituents (terpenes) in the essential oils obtained from Tagetes lucida, Cymbopogon citratus, Lippia alba and Eucalyptus citriodora, their epoxidation with DMDO in acetone solution and the characterization of the resulting epoxides by GC-MS (EI) and NMR. This is one of the first reports involving the application of dioxirane chemistry to essential oils in order to generate modified compounds with potential uses in several areas of medicine and industry.

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