Associations of aHinfI PCR-RFLP ofPOU1F1Gene with Growth Traits in Qinchuan Cattle

2009 ◽  
Vol 20 (2) ◽  
pp. 71-74 ◽  
Author(s):  
Chunlei Zhang∗ ◽  
Bo Liu∗ ◽  
Hong Chen ◽  
Xianyong Lan ◽  
Chuzhao Lei ◽  
...  
Keyword(s):  
Growth Traits ◽  
Qinchuan Cattle ◽  
Pcr Rflp

Exploring polymorphisms and associations of the bovine MOGAT3 gene with growth traits

Genome ◽  
2012 ◽  
Vol 55 (1) ◽  
pp. 56-62 ◽  
Author(s):  
Jiajie Sun ◽  
Chunlei Zhang ◽  
Xianyong Lan ◽  
Chuzhao Lei ◽  
Hong Chen
Keyword(s):  
Growth Traits ◽  
Enzymatic Reaction ◽  
Fatty Acyl ◽  
Missense Mutations ◽  
Coding Region ◽  
Dna Pooling ◽  
Qinchuan Cattle ◽  
Rate Limiting Step ◽  
Pcr Rflp ◽  
Acyl Coenzyme A

Monoacylglycerol acyltransferase (MGAT3, also known as MOGAT3) catalyzes the synthesis of diacylglycerol (DAG) using 2-monoacylglycerol and fatty acyl coenzyme A. This enzymatic reaction is believed to be an essential and rate-limiting step for the absorption of dietary fat in the small intestine. However, similar research for the bovine MOGAT3 gene is lacking. Therefore, in this paper, polymorphisms of the bovine MOGAT3 gene were detected in 1145 individuals from five cattle breeds by DNA pooling, PCR–RFLP, and DNA sequencing methods. The results showed that 26 novel SNPs were identified, which included 16 mutations in the coding region and the others in the introns. Additionally, association analysis between two missense mutations, g.A229G and g.G1627A, and growth traits in Nanyang cattle up to 2 years of age and adult Qinchuan cattle was performed. The results indicated that polymorphisms were significantly associated with Nanyang cattle, but no convincing associations were observed for Qinchuan cattle for the studied traits.

scholarly journals Melanocortin-4 Receptor (MC4R) gene polymorphism and its effect on growth traits in Madura cattle

2019 ◽  
Vol 44 (1) ◽  
pp. 38
Author(s):  
P. W. Prihandini ◽  
S. Sumadi ◽  
G. Suparta ◽  
D. Maharani
Keyword(s):  
Growth Traits ◽  
Balance Control ◽  
Direct Sequencing ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Mc4r Gene ◽  
Melanocortin 4 Receptor ◽  
Pcr Rflp ◽  
Reverse Primer ◽  
Selection For

Melanocortin-4 receptor (MC4R) gene has an important role in the regulation of feed intake and energy balance control. The objective of this study was to identify the single nucleotide polymorphisms (SNPs) of MC4R gene and their association with growth traits in Madura cattle. A total of 198 calves were used in this study.Forward primer: 5’-GTCGGGCGTCTTGTTCATC-3’and reverse primer: 5’-GCTTGTGTTTAGCATCGCGT-3’ were used to amplify approximately 493 bp of MC4R gene. The results showed that two SNPs, g.1133C>G and g.1108C>T were identified by direct sequencing. The PCR-RFLP method was performed to genotype all individuals studied based on SNP g.1133C>G, and its SNP was significantly associated with shoulder height (SH) at yearling age (P<0.05). Animals with GG genotype had a higher SH (110.35±6.40cm) than those with CC (102.00±8.00 cm) and CG genotype (105.96±6.23 cm). The SNP g.1133 C>G changed amino acid from valine to leucine. In conclusion, the SNP g.1133C>G of the MC4R gene may be used as a marker-assisted selection for SH trait in Madura cattle.

scholarly journals Genetic variants in MYF5 affected growth traits and beef quality traits in Chinese Qinchuan cattle

Genomics ◽  
2020 ◽  
Vol 112 (4) ◽  
pp. 2804-2812 ◽  
Author(s):  
Chunping Zhao ◽  
Sayed Haidar Abbas Raza ◽  
Rajwali Khan ◽  
Ahmed Sabek ◽  
Samiullah Khan ◽  
...  
Keyword(s):  
Genetic Variants ◽  
Growth Traits ◽  
Quality Traits ◽  
Beef Quality ◽  
Qinchuan Cattle

scholarly journals Expression profiles of the MXD3 gene and association of sequence variants with growth traits in Xianan and Qinchuan cattle

2020 ◽  
Vol 6 (3) ◽  
pp. 399-409 ◽  
Author(s):  
Dan Hao ◽  
Bo Thomsen ◽  
Jiangsong Bai ◽  
Shujun Peng ◽  
Xianyong Lan ◽  
...  
Keyword(s):  
Growth Traits ◽  
Expression Profiles ◽  
Sequence Variants ◽  
Qinchuan Cattle

SNP and haplotype analysis reveal IGF2 variants associated with growth traits in Chinese Qinchuan cattle

2013 ◽  
Vol 41 (2) ◽  
pp. 591-598 ◽  
Author(s):  
Yong-Zhen Huang ◽  
Zhao-Yang Zhan ◽  
Xin-Yi Li ◽  
Sheng-Ru Wu ◽  
Yu-Jia Sun ◽  
...  
Keyword(s):  
Haplotype Analysis ◽  
Growth Traits ◽  
Qinchuan Cattle

scholarly journals Polymorphism Exploration of Growth Family (GH, GHRH and PIT-1) Genes Polymorphisms of Local Swamp Buffalo for Productivity Improvement in North Tapanuli Regency, North Sumatra

2021 ◽  
Vol 33 ◽  
pp. 02005
Author(s):  
Anneke Anggraeni ◽  
Chalid Thalib ◽  
Angga Ardhati Rani Hapsari
Keyword(s):  
Growth Traits ◽  
Restriction Enzymes ◽  
Economic Traits ◽  
Productivity Improvement ◽  
Pcr Rflp ◽  
Gh Gene ◽  
Molecular Selection ◽  
Swamp Buffalo ◽  
Livestock Productivity ◽  
North Sumatra

Genetic improvement of livestock productivity can be done through molecular selection on the genes controlling growth traits. Genetic polymorphism of the growth family (GH, GHRH, and PIT1) genes were studied in local swamp buffalo (106 hds.) from a government buffalo breeding station (46 heads) and smallholders (60 heads) in North Tapanuli District, North Sumatra Province. Genotype variants of the three genes were identified by PCR-RFLP method using restriction enzymes of MspI (GH gene), HaeIII (GHRH gene) and HinfI (PIT -1 gene). Genotyping on individual GH_g.1547T>C, GHRH_g.4666G>C, and PIT -1_g.1256G >A loci resulted only one type genotype, respectively TT, CC, an d AA, with one type of allele, respectively T, C, and A. Heterozygosity observation (Ho) and expectation (He) values values and the PIC value for each locus was 0.00. It could be suggested to increase genotype frequenciest of the three growth genes that are positively associated with the growth traits and economic traits of the buffalo.

scholarly journals Exploring insertions and deletions (indels) of <i>MSRB3</i> gene and their association with growth traits in four Chinese indigenous cattle breeds

2019 ◽  
Vol 62 (2) ◽  
pp. 465-475 ◽  
Author(s):  
Mingli Wu ◽  
Shipeng Li ◽  
Guoliang Zhang ◽  
Yingzhi Fan ◽  
Yuan Gao ◽  
...  
Keyword(s):  
Growth Traits ◽  
Methionine Sulfoxide ◽  
Methionine Sulfoxide Reductase ◽  
Qinchuan Cattle ◽  
Cattle Breeding ◽  
Indigenous Cattle ◽  
Insertions And Deletions ◽  
Cattle Breeds ◽  
Dna Pool ◽  
Candidate Loci

Abstract. Methionine sulfoxide reductase B3 (MSRB3) is instrumental in ossification and fat deposition, which regulate the growth and development of cattle directly. The purpose of this study was aimed to explore insertions and deletions (indels) in MSRB3 gene and investigate their association with growth traits in four indigenous cattle breeds (Luxi cattle, Qinchuan cattle, Nanyang cattle, and Jiaxian Red cattle). Four indels were identified by sequencing with DNA pool. Association analysis showed that three of them were associated with growth traits (P<0.05). For P1, the DD (deletion and deletion) genotype was significantly associated with body length of Nanyang cattle; for P6, II (insertion and insertion) and/or DD genotypes were significantly associated with enhanced growth traits of Qinchuan cattle; for P7, II genotype was significantly associated with hucklebone width of Luxi cattle. Our results demonstrated that the polymorphisms in bovine MSRB3 gene were significantly associated with growth traits, which could be candidate loci for marker-assisted selection (MAS) in cattle breeding.

scholarly journals Genetic Polymorphisms of Cyp19 and Myostatin Genes in Turkish Indigenous Sheep Breeds

2018 ◽  
Vol 68 (3) ◽  
pp. 313
Author(s):  
I. AKIŞ ◽  
F. ESEN GÜRSEL ◽  
N. HACIHASANOĞLU ÇAKMAK ◽  
G. ATMACA ◽  
H. YARDIBI ◽  
...  
Keyword(s):  
Growth Traits ◽  
Muscle Growth ◽  
Reproductive Activity ◽  
Meat Production ◽  
Production Traits ◽  
Sheep Breeds ◽  
Indigenous Sheep ◽  
Estrogen Synthesis ◽  
Pcr Rflp ◽  
Cyp19 Gene

Growth and meat production traits are very important in sheep breeding. Cyp19 gene has a major role in reproductive activity and growth due to its function in estrogen synthesis. Another gene affecting growth traits is  Myostatin (MSTN) gene, which mainly regulates skeletal muscle growth. In this study allele frequencies of genetic polymorphism in Cyp19 and Myostatin genes were identified by PCR-RFLP method in five indigenous Turkish sheep breeds, Chiose, Imroz, Kivircik, Zom and Morkaraman. Digestion of Cyp19 gene with HaeIII only revealed uncut AA genotype and digestion of MSTN with DraI also revealed only uncut AA genotype. Both loci analyzed in this study were found to be monomorphic in five Turkish indigenous sheep breeds. These highly conserved parts of the two genes can be useful for molecular evolutionary studies in sheep. Further studies regarding association analysis of Cyp19 and MSTN in sheep should be conducted.

scholarly journals Association of PIT1 genotypes with growth traits in Canchim cattle

2008 ◽  
Vol 65 (2) ◽  
pp. 116-121 ◽  
Author(s):  
Sônia Mara Carrijo ◽  
Maurício Mello de Alencar ◽  
Fábio Luiz Buranelo Toral ◽  
Luciana Correia de Almeida Regitano
Keyword(s):  
Least Squares Method ◽  
Growth Traits ◽  
Daily Weight Gain ◽  
Favorable Effect ◽  
Genotype Effect ◽  
Genetic Groups ◽  
Pcr Rflp ◽  
Method Effects ◽  
The Difference ◽  
Trait Locus

Use of genetic markers can aid on the identification of animals with highest breeding values in beef cattle. The PIT1 gene codes for the Pit-1 transcription factor is essential for the activation of prolactin, growth hormone and PIT1 genes. This research is an investigation of the effect of PIT1-HinfI polymorphism on growth traits of 509 Canchim animals, from two lineages, GG1 and GG2. PIT1 genotypes were identified through PCR-RFLP. Genotype effect on phenotypic values for birth weight (BW), standardized weaning weight (W240), weight at 12 months of age (W365), and the average daily weight gain from birth to weaning (AGBW), and from weaning to 12 months of age (AGW12) were analyzed by the least squares method. Effects of the interaction between the animal's genetic group and PIT1 genotype for W240, AGBW and AGW12 were observed (P < 0.05). Differences between means of HinfI (-/-), HinfI (+/+) and HinfI (+/-) genotypes for W240 and AGBW were observed in GG2 (P < 0.05), revealing superiority of (-/-) genotype for those traits. Means for genotypes (+/+) and (+/-) for W240 and AGBW, did not differ from one another, suggesting a dominance effect of the HinfI (+) allele. The HinfI (-) allele had a favorable effect on W240 and AGBW in GG2, when present in homozygosis. The difference between PIT1 behavior observed in the two genetic groups may suggest the action of a quantitative trait locus linked to PIT1, segregating only in GG2 population.

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