Exploring polymorphisms and associations of the bovine MOGAT3 gene with growth traits

Monoacylglycerol acyltransferase (MGAT3, also known as MOGAT3) catalyzes the synthesis of diacylglycerol (DAG) using 2-monoacylglycerol and fatty acyl coenzyme A. This enzymatic reaction is believed to be an essential and rate-limiting step for the absorption of dietary fat in the small intestine. However, similar research for the bovine MOGAT3 gene is lacking. Therefore, in this paper, polymorphisms of the bovine MOGAT3 gene were detected in 1145 individuals from five cattle breeds by DNA pooling, PCR–RFLP, and DNA sequencing methods. The results showed that 26 novel SNPs were identified, which included 16 mutations in the coding region and the others in the introns. Additionally, association analysis between two missense mutations, g.A229G and g.G1627A, and growth traits in Nanyang cattle up to 2 years of age and adult Qinchuan cattle was performed. The results indicated that polymorphisms were significantly associated with Nanyang cattle, but no convincing associations were observed for Qinchuan cattle for the studied traits.

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Haplotype combination of the bovine INSIG1 gene sequence variants and association with growth traits in Nanyang cattle

Genome ◽

10.1139/g2012-029 ◽

2012 ◽

Vol 55 (6) ◽

pp. 429-436 ◽

Cited By ~ 3

Author(s):

Jiajie Sun ◽

Yuan Gao ◽

Dong Liu ◽

Wei Ma ◽

Jing Xue ◽

...

Keyword(s):

Gene Sequence ◽

Growth Traits ◽

Regulatory Element ◽

Sequence Variants ◽

Coding Region ◽

Dna Pooling ◽

Proteolytic Activation ◽

Haplotype Combination ◽

Pcr Rflp ◽

Sterol Regulatory Element

The insulin-induced gene 1 (INSIG1) gene encodes a protein that blocks proteolytic activation of sterol regulatory element binding proteins, which are transcription factors that activate genes that regulate cholesterol, fatty acid, and glucose metabolism. However, similar research for the bovine INSIG1 gene is lacking. Therefore, in this study, polymorphisms of the bovine INSIG1 gene were detected in 643 individuals from four cattle breeds by DNA pooling, forced PCR–RFLP, PCR–SSCP, and DNA sequencing methods. Only 10 novel SNPs were identified, which included four mutations in the coding region and the others in the introns. In Nanyang individuals, seven common haplotypes were identified based on four coding region SNPs. The haplotype GACT, with a frequency of 75.4%, was the most prevalent haplotypes and SNPs formed two linkage disequilibrium blocks with strong multi-allelic D′ (D′ = 1). Additionally, association analysis between mutations of the bovine INSIG1 gene and growth traits in Nanyang cattle at 6, 12, 18, and 24 months old was performed, and the results indicated that the polymorphisms were not significantly associated with body mass.

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Associations of aHinfI PCR-RFLP ofPOU1F1Gene with Growth Traits in Qinchuan Cattle

Animal Biotechnology ◽

10.1080/10495390802640462 ◽

2009 ◽

Vol 20 (2) ◽

pp. 71-74 ◽

Cited By ~ 12

Author(s):

Chunlei Zhang∗ ◽

Bo Liu∗ ◽

Hong Chen ◽

Xianyong Lan ◽

Chuzhao Lei ◽

...

Keyword(s):

Growth Traits ◽

Qinchuan Cattle ◽

Pcr Rflp

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Six novel coding SNPs of the nucleophosmin 1 (<i>NPM1</i>) gene and their associations with growth traits in bovine (Brief Report)

Archives Animal Breeding ◽

10.5194/aab-53-368-2010 ◽

2010 ◽

Vol 53 (3) ◽

pp. 368-371 ◽

Cited By ~ 13

Author(s):

Y. Huang ◽

E. Zhang ◽

Z. Li ◽

J. Wang ◽

Y. Huai ◽

...

Keyword(s):

Cell Growth ◽

Ribosome Biogenesis ◽

Cell Cycle Progression ◽

Growth Traits ◽

Chromosome 9 ◽

Coding Region ◽

Mouse Development ◽

Cycle Progression ◽

Gene Coding ◽

Pcr Rflp

Abstract. The Nucleophosmin 1 (NPM1) gene encodes a multifunctional nucleolar phosphoprotein that has crucial roles in the control of different aspects of cell growth and homeostasis, such as ribosome biogenesis, centrosome duplication, cell cycle progression, apoptosis and cell differentiation (GRISENDI et al. 2006, NAOE et al. 2006). As mutants of NPM1 gene impact protein synthesis, NPM1 is an essential protein in mouse development and cell growth (MAGGI et al. 2008). The bovine NPM1 gene contains one exon and locates at chromosome 9. In previous work, the 12-bp deletion was detected in bovine NPM1 gene coding region. (HUANG et al. 2010). In this study, the coding region of bovine NPM1 gene has been scanned by PCR-SSCP, DNA sequencing and forced PCR-RFLP methods for SNPs in 1 032 individuals belonging to four Chinese cattle breeds. Association of six mutations of NPM1 gene with growth traits was analyzed.

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Transcriptional Regulation by the Short-Chain Fatty Acyl Coenzyme A Regulator (ScfR) PccR Controls Propionyl Coenzyme A Assimilation by Rhodobacter sphaeroides

Journal of Bacteriology ◽

10.1128/jb.00402-15 ◽

2015 ◽

Vol 197 (19) ◽

pp. 3048-3056 ◽

Cited By ~ 11

Author(s):

Michael S. Carter ◽

Birgit E. Alber

Keyword(s):

Rhodobacter Sphaeroides ◽

Coenzyme A ◽

Bacterial Species ◽

Fatty Acyl ◽

Short Chain ◽

Coding Region ◽

Acetyl Coa ◽

Content Type ◽

Chain Acyl ◽

Acyl Coenzyme A

ABSTRACTPropionyl coenzyme A (propionyl-CoA) assimilation byRhodobacter sphaeroidesproceeds via the methylmalonyl-CoA pathway. The activity of the key enzyme of the pathway, propionyl-CoA carboxylase (PCC), was upregulated 20-fold during growth with propionate compared to growth with succinate. Because propionyl-CoA is an intermediate in acetyl-CoA assimilation via the ethylmalonyl-CoA pathway, acetate growth also requires the methylmalonyl-CoA pathway. PCC activities were upregulated 8-fold in extracts of acetate-grown cells compared to extracts of succinate-grown cells. The upregulation of PCC activities during growth with propionate or acetate corresponded to increased expression of thepccBgene, which encodes a subunit of PCC. PccR (RSP_2186) was identified to be a transcriptional regulator required for the upregulation ofpccBtranscript levels and, consequently, PCC activity: growth substrate-dependent regulation was lost whenpccRwas inactivated by an in-frame deletion. In thepccRmutant,lacZexpression from a 215-bp plasmid-bornepccBupstream fragment including 27 bp of thepccBcoding region was also deregulated. A loss of regulation as a result of mutations in the conserved motifs TTTGCAAA-X4-TTTGCAAA in the presence of PccR allowed the prediction of a possible operator site. PccR, together with homologs from other organisms, formed a distinct clade within the family ofshort-chainfatty acyl coenzyme Aregulators (ScfRs) defined here. Some members from other clades within the ScfR family have previously been shown to be involved in regulating acetyl-CoA assimilation by the glyoxylate bypass (RamB) or propionyl-CoA assimilation by the methylcitrate cycle (MccR).IMPORTANCEShort-chain acyl-CoAs are intermediates in essential biosynthetic and degradative pathways. The regulation of their accumulation is crucial for appropriate cellular function. This work identifies a regulator (PccR) that prevents the accumulation of propionyl-CoA by controlling expression of the gene encoding propionyl-CoA carboxylase, which is responsible for propionyl-CoA consumption byRhodobacter sphaeroides. Many otherProteobacteriaandActinomycetalescontain one or several PccR homologs that group into distinct clades on the basis of the pathway of acyl-CoA metabolism that they control. Furthermore, an upstream analysis of genes encoding PccR homologs allows the prediction of conserved binding motifs for these regulators. Overall, this study evaluates a single regulator of propionyl-CoA assimilation while expanding the knowledge of the regulation of short-chain acyl-CoAs in many bacterial species.

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Haplotype combination of SREBP-1c gene sequence variants is associated with growth traits in cattle

Genome ◽

10.1139/g11-016 ◽

2011 ◽

Vol 54 (6) ◽

pp. 507-516 ◽

Cited By ~ 42

Author(s):

Yong-Zhen Huang ◽

Hua He ◽

Jia-Jie Sun ◽

Jing Wang ◽

Zhuan-Jian Li ◽

...

Keyword(s):

Growth Traits ◽

Regulatory Element ◽

Large Deletion ◽

Silent Mutation ◽

Sequence Variants ◽

Missense Mutations ◽

Cattle Breeds ◽

Pcr Rflp ◽

Element Binding Protein ◽

Strong Candidate

The aim of this study was to examine the association of the SREBP-1c polymorphism with growth traits in cattle breeds. Five sequence variants (SVs) were identified within the bovine sterol regulatory element-binding protein-1c gene (SREBP-1c), using DNA sequencing, PCR, PCR–RFLP, and forced PCR–RFLP methods. These polymorphisms include three missense mutations (SV1, SV4, and SV5) in exons 7, 9, and 12, a silent mutation (SV3) in exon 9, and a large deletion (SV2) in intron 7. Overall, we report the validation of polymorphisms within the bovine SREBP-1c gene, and the haplotype variability and extent of linkage disequilibrium (LD) in 1061 individuals representing the five main cattle breeds from China. We also investigated haplotype frequencies and LD coefficients for five SVs in all study populations. LD and haplotype structure of SREBP-1c were different between breeds. The result of haplotype analysis of five SVs showed that 27 different haplotypes were identified by all breeds. Two haplotypes (Hap1 and Hap2) shared by all five populations accounted for 42.75%, 35.68%, 36.44%, 25.43%, and 96.26% of all haplotypes observed in the cattle breeds Nanyang, Qinchuan, Jiaxian, Jinnan, and Chinese Holstein, respectively. The statistical analyses indicated that one single SV and 38 combined haplotypes were significantly associated with growth traits in the Nanyang cattle population (P < 0.05 or P < 0.01). The results of this study suggest that the SREBP-1c gene possibly is a strong candidate gene that affects growth traits in the Chinese beef cattle breeding program.

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Comparative analysis of the IGF2 and ZBED6 gene variants and haplotypes reveals significant effect of growth traits in cattle

Genome ◽

10.1139/gen-2013-0062 ◽

2013 ◽

Vol 56 (6) ◽

pp. 327-334 ◽

Cited By ~ 4

Author(s):

Yong-Zhen Huang ◽

Zhao-Yang Zhan ◽

Yu-Jia Sun ◽

Jing Wang ◽

Ming-Xun Li ◽

...

Keyword(s):

Body Mass ◽

Growth Traits ◽

Muscle Growth ◽

Missense Mutations ◽

Nucleotide Polymorphisms ◽

Coding Region ◽

Mutant Type ◽

Cattle Breeds ◽

Proliferation And Differentiation ◽

Different Ages

Muscle growth is a complex phenomenon regulated by many factors, whereby net growth results from the combined action of synthesis and turnover. Insulin-like growth factor 2 (IGF2) is a fetal growth and differentiation factor that plays an important role in muscle growth and in myoblast proliferation and differentiation; Zinc finger, BED-type containing 6 (ZBED6) is a novel transcription factor that was identified and shown to act as a repressor of IGF2 transcription in skeletal muscle. In this study, a total of seven single nucleotide polymorphisms (SNPs) were identified, four SNPs in intron 8 of IGF2 and one promoter SNP and two missense mutations in the coding region of ZBED6, two of which were in complete linkage disequilibrium (LD) in the bovine IGF2. The 58 haplotypes were inferred in 1522 individuals representing four purebred cattle breeds from China. The seven SNPs, 79 and 66 combined diplotypes were revealed for association with body mass in Nanyang and Jiaxian cattle populations at five different ages (P < 0.05 or 0.01). The mutant-type variants and haplotype 58 (likely in LD with the beneficial quantitative trait nucleotide allele) was superior for body mass; the heterozygote diplotype of the most common haplotypes 58 was associated with higher body mass compared to either heterozygote or homozygote. The statistical analyses indicated that the mutant-type variants and haplotypes are significantly associated with body mass in study cattle populations at different ages. These data demonstrate that variants and haplotypes are associated with growth traits, and these results may provide important biological insights into the phenotypic differentiation that is associated with adaptation and specialization of cattle breeds.

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