Glucocorticoid Regulates the Synthesis of Porcine Muscle Protein through m6A Modified Amino Acid Transporter SLC7A7

The occurrence of stress is unavoidable in the process of livestock production, and prolonged stress will cause the decrease of livestock productivity. The stress response is mainly regulated by the hypothalamic-pituitary-adrenal axis (HPA axis), which produces a large amount of stress hormones, namely glucocorticoids (GCs), and generates a severe impact on the energy metabolism of the animal body. It is reported that m6A modification plays an important role in the regulation of stress response and also participates in the process of muscle growth and development. In this study, we explored the effect of GCs on the protein synthesis procession of porcine skeletal muscle cells (PSCs). We prove that dexamethasone affects the expression of SLC7A7, a main amino acid transporter for protein synthesis by affecting the level of m6A modification in PSCs. In addition, we find that SLC7A7 affects the level of PSC protein synthesis by regulating the conduction of the mTOR signaling pathway, which indicates that the reduction of SLC7A7 expression may alleviate the level of protein synthesis under stress conditions.

Physiological Response and Production Ability of Beef Cattle Raised Based on Different Altitudes in Sinjai Regency

The breeders' knowledge of the suitability of the breeding environment for cattle breeds may increase livestock productivity. Determining the differences in microclimate environmental conditions, physiological responses, and production capabilities of Bali and Limousine crossbred cattle raised at different altitudes in Sinjai Regency were the aim of this study. The research was carried out on people's farms in Sinjai, which were divided into three locations based on the height of different rearing places; low areas (0-100 masl) were represented by East Sinjai sub-district, South Sinjai sub-district for medium plains areas (100-400 masl), and highland areas (>400 masl) were represented by West Sinjai sub-district. The selection was based on the height of the place and beef cattle business in the area. The materials used were 30 Balinese cattle and 24 Limousine hybrids. This research was conducted from July to September 2020. The method of the research used direct observation in the field. Data collection used the method of direct observation in the field. The parameters observed were; microclimate conditions, physiological responses, and beef cattle production capabilities. The research found that the altitude of keeping Bali cattle and Limousine breeding affected the microclimate conditions such as air temperature and humidity in the highlands. The air temperature is lower in the highlands but the humidity is higher than the lowlands. Besides, on the physiological response parameters, the altitude effected on the Limousine hybrid as well as respiratory frequency and pulse rate of Bali cattle. Respiratory and pulse rates are lower at high altitudes. The height of the breeding ground also affected the height of the Bali cattle, and the chest circumference of the Limousine hybrid.

Performance of Urochloa and Megathyrsus Forage Grasses in Smallholder Farms in Western Kenya

Livestock productivity has remained low in sub-Saharan African countries compared to other places on the globe. The feeding component is the major limitation, in both quantity and quality. Among other inputs, feeding takes 55–70% of the costs involved. Livestock play a major role especially in smallholder mixed farms through provision of household nutrition and income through milk and meat. Equally, fertilization of cropland benefits from livestock manure, and livestock often act as insurance and savings by providing liquidity for unforeseen and urgent financial needs. Increasing livestock productivity would enhance the fore-mentioned benefits contributing to well-being and livelihoods. Toward this endeavor and with smallholder dairy farmers' participation, we undertook an evaluation of 10 selected forages from Urochloa Syn. Brachiaria and Megathyrsus syn. Panicum genus and compared them with Napier grass, i.e., Cenchrus purpureus Syn. Pennisetum purpureum commonly grown by farmers. For detailed and robust evaluation, we established the species in eight trial sites spread in four administrative counties in Western Kenya (Bungoma, Busia, Kakamega, and Siaya). In each site, the forages were established in plots in a randomized complete block design, replicated three times. Each site was linked to a group of farmers interested in dairy. For 2 years, dry matter production, plant height, and leaf-to-stem ratio was determined across all sites. Further, we guided farmers to generate participatory forage evaluation criteria, which they later administered across their respective forage demonstration sites individually on plot-by-plot basis to generate preference rating compared to what they normally grow—Napier grass. The results showed significant differences across the forage types within and between the sites. Cumulative dry matter yields ranged 13.7–49.9 t/ha over 10 harvestings across forage types and the counties, while values for crude protein were 1.85–6.23 t/ha and 110,222–375,988 MJ/ha for metabolizable energy. Farmer preferences emerged that highlighted forages with likely better chances of adoption with weighed scores ranging 5.5–7.6 against a scale of 1–9, across the counties. The observations provide additional and well-performing forage options for the farmers and possibly in similar production systems and ecologies. Awareness creation targeting livestock and dairy producers would be key, reaching, and informing them on alternative forage options, with potential to increase livestock productivity.

Analysis of PT SH's Feed Additive Marketing Strategy Based on Competitive Setting Profile (CSP) and Company Alignment Profile (CAP)

PT SH is a company that distributes veterinary medicinal products produced by Lallemand Animal Nutrition (France) and several products from other suppliers. This product from Lallemand is included in the feed additive class which functions to increase livestock productivity with better feed conversion. Competition in product sales in this group greatly affects the sales of feed additives for PT SH. Therefore, this study uses CAP and CSP analysis to analyze the marketing of PT SH, and analyzes the competition between PT SH and other companies in the sale of feed additives. CAP analysis consists of marketing strategy, tactics and values, while CSP analysis consists of customer demand, competitive situation, and variable factors. CAP analysis gives an average score of 2.82, this mid score indicates that the strategies and tactics used as well as the marketing value of PT SH need to be improved in order to increase PT SH's feed additive sales. CSP analysis gives an average score of 3.56, this fairly large score indicates that PT SH's competition with other companies is quite strong in the sale of feed additives. This requires PT SH to maintain quality, improve marketing strategy services to increase sales of feed additives, so that PT SH is not left behind by other companies. Keywords: CAP analysis, CSP analysis, Mix, Feed additive, Marketing.

Ecology as the main way for sustainable development in agriculture

Based on these facts, it is clear that agriculture has a detrimental impact on the environment at this stage of development. Taking into account the negative consequences of its activities, it can be concluded that, in today’s world, there should be tendencies to create ecological equilibrium, soil recovery, restoration of biogeochemical cycles, improvement of the resilience of agro-ecosystems to ensure the cleanliness of all agricultural products. Their successful solution can only be based on environmental management, a comprehensive system of measures to conserve wildlife, and increased agricultural and livestock productivity. Positioning Russia as a country with good and clean lands for the production of food materials, with rich genetic resources, the good environmental situation in many agricultural regions will contribute to the creation of a favorable investment climate in such industries as ecological agriculture, production of environmentally friendly products, development of environmental, agricultural, and gastronomic tourism, service and restaurant sectors. Making the ecologization of agriculture a priority will help to address problems such as increasing soil fertility at the expense of soil-forming organisms, preserving the biodiversity of plants, animals, increasing the sustainability of agro- and biocenoses and it will ultimately lead to the restoration and preservation of human health.

PENINGKATAN PRODUKTIVITAS SAPI POTONG MENGGUNAKAN PROBIOTIK UNTUK RUMINANSIA DI DESA WONGSOREJO DAN GOMBENGSARI KECAMATAN WONGSOREJO, KABUPATEN BANYUWANGI, JAWA TIMUR

The Banyuwangi Regency Government, through the Livestock Service Office, is very active in improving the community's economic level through the livestock sector. It is proven through a variety of dynamic population numbers in 2014 namely, 108,139 beef cattle. For this reason, it is necessary to get broad attention in terms of increasing the productivity of livestock. The success of increasing livestock productivity is determined by the factors of seed, feed, and management, while the largest component, which is 70%, comes from the feed factor. So far, it is rarely known that forage feed or additional feed in the form of tofu dregs or concentrate, is not completely digested in the small intestine but only about 60-70%, while the rest is excreted through feces and urine. For this reason, it is necessary to provide feed supplementation in the form of probiotics for ruminants containing several non-pathogenic microorganisms (bacteria) that can help increase feed digestibility. Probiotics can be defined as food supplements containing live microbes that have a beneficial effect on animals as their host through improving the balance of microflora in the digestive tract. Community service activities that have been carried out include the provision of probiotics for ruminants which are given directly to farmers by visiting the location of the beef cattle pen. The community service program is carried out for 5 months including counseling, training, evaluation, and cadre development. It can be concluded that there is an increase in cognitive, affective, psychomotor and substance aspects in the partner community.Keywords: beef cattle; Wongsorejo; Gombengsari; Banyuwangi AbstrakPemerintah Kabupaten Banyuwangi, melalui Dinas Peternakan sangat gencar dalam meningkatkan taraf perekonomian masyarakat melalui sektor peternakan. Terbukti melalui beragam jumlah populasi dinamis pada tahun 2014 yakni, sapi potong 108.139 ekor. Untuk itu perlu mendapat perhatian yang luas dalam hal peningkatan produktivitas hewan ternak. Keberhasilan peningkatan produktivitas peternakan ditentukan oleh faktor bibit, pakan dan pengelolaan, sedangkan komponen terbesar yaitu sebesar 70 % berasal dari faktor pakan. Selama ini jarang diketahui bahwa pakan hijauan maupun pakan tambahan berupa ampas tahu atau konsentrat, tidak seluruhnya dicerna dalam usus halus melainkan hanya sekitar 60-70 %,sedangkan yang sisanya dibuang lewat feses dan urine. Untuk itu perlu dilakukan pemberian suplementasi pakan berupa pro biotik untuk Ruminan yang mengandung beberapa mikroorganisme (bakteri) non patogen yang mampu membantu peningkatan kecernaan pakan. Probiotik dapat didefinisikan sebagai suplemen makanan yang mengandung mikrobial hidup menimbulkan efek menguntungkan hewan sebagai induk semangnya melalui peningkatan keseimbangan mikroflora di dalam saluran pencernaan. Kegiatan pengabdian kepada masyarakat yang telah dilakukan meliputi pemberian probiotik untuk ruminan yang diberikan secara langsung kepada peternak dengan mendatangi lokasi kandang sapi pedaging. Program pengabdian masyarakat dilakukan selama 5 bulan meliputi penyuluhan, pelatihan, evaluasi, dan pembinaan kader. Dapat disimpulkan terdapat peningkatan aspek kognitif, afektif, psikomotor dan substansi pada masyarakat mitra.Kata kunci: sapi potong; Wongsorejo; Gombengsari; Banyuwangi

Designing and Simulating the Control Process of a Feed Pusher Robot Used on a Dairy Farm

Feeding cattle on livestock farms is a labor-intensive operation that requires considerable capital investments to purchase equipment and cover labour costs. The global trends in developing technological equipment for feeding cattle include the robotization of various processes. The operation of feed pushing on the feeding table is an integral part of the feeding process, which has a significant impact on livestock productivity. This study concentrates on the simulation modeling of a feed pusher robot using Simulink tools in the Matlab environment to facilitate robot modernization or optimize the final cost for artificial testing of typical system elements and reduce production costs. Based on a simulation model, an experimental sample was designed with a controlled dispenser of feed additives, which can significantly facilitate the feeding process and optimize the dosing of concentrated additives.

Strategy analysis of extension of performance in transfer of livestock technology innovation for farmer empowerment

The impact of the Covid-19 pandemic is still widespread and needs to be watched out for. In government policies that implement the new normal in several places, agricultural extension workers play a very important strategic role in responding to the pandemic, especially in rural areas. This study aims to analyze the performance strategy of extension workers in assisting farmers during the pandemic in disseminating information on livestock technology innovations. The research was conducted in Barru Regency, with the sub-district location being the sample. Sources of data in this study were collected using surveys/interviews with extension workers, farmers, stakeholders, and in-depth interviews with informants. The results show that the performance strategy that can be taken in improving the performance of the extension worker in the transfer of livestock technology innovation is by increasing the competence of the instructor in the guidance carried out to overcome various problems during the implementation of the extension program and improve innovation, communication and extension methods according to the needs of farmers and support from government agencies. Therefore, it is expected to improve the performance of agricultural extension workers by increasing the extension budget and improving extension facilities and infrastructure so that farmers can adopt agricultural/livestock technology innovations provided by extension workers to increase livestock productivity, increase farmer income and welfare.

The addition of feed additive in beef cattle ration on in vitro fermentation characteristics

The effects of feed additives are increasing feed digestibility, balance of rumen microbial community, stimulating the immune response and livestock productivity. This study aimed to determine the effects of feed additives combination in the rumen fermentation. The method used in this study was a block randomized design with 9 treatments and 3 replications. The experiment using Theodorou In vitro method for 48 hours with parameters such as pH, kinetics gas and methane production, DMD, OMD, NH3, and partial VFA. The treatment were P0; control (Basal Diet 70% Concentrate + 30% Forages), P1; P0 + Premix, P2; P1 + Probiotic, P3; P1 + Enzyme, P4; P1 + Plant Extract, P5; P1 + (Probiotics + Enzyme), P6; P1 + (Probiotics + Plant Extract), P7; P1 + (Probiotic + Enzyme + Plant Extract), and P8; P1 + (Enzyme + Plant Extract). The results showed kinetics gas, methane production, NH3, and partial VFA were significantly affected (P <0.05), while the value of pH, DMD, and OMD was not significant. In conclusion, the addition of mix feed additives can affect the kinetics gas and methane production. However, it does not affect the level of pH, dry matter and organic matter digestibility.