Improved HPTLC Method for Determination of Curcuminoids from Curcuma longa

Curcumin, an important phytoconstituent obtained from Curcuma longa L. (turmeric) is used traditionally in the treatment of various inflammatory diseases like arthritis, stroke and bowel diseases, etc. Despite its many health benefits, instability of curcumin in plasma is a major issue. The retention of curcumin in plasma must be properly evaluated in order to establish its stability in biological systems. The current study presents an HPTLC method undertaken for detection of curcumin and determination of its stability in plasma and different tissues of rats. The plasma and tissue samples were appropriately processed to render them suitable for HPTLC analysis. The method employed HPTLC glass plates precoated with silica gel 60F254 as the stationary phase. The mobile phase developed consisted of chloroform, methanol and glacial acetic acid which successfully gave distinct bands for curcumin with a Rf value of 0.95. This newly developed HPTLC method was found to be reproducible and accurate in quantifying curcumin in mammalian samples. This method was further utilized to efficiently estimate the time for which curcumin is retained in its native form in mammalian tissues and plasma alike.

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HPTLC Method for the Quantitative Determination of ar-Turmerone and Turmerone in Lipid Soluble Fraction from Curcuma longa

Natural Product Communications ◽

10.1177/1934578x0700200912 ◽

2007 ◽

Vol 2 (9) ◽

pp. 1934578X0700200 ◽

Cited By ~ 2

Author(s):

Vikas Jain ◽

Vure Prasad ◽

Satwayan Singh ◽

Raghwendra Pal

Keyword(s):

Quantitative Determination ◽

High Performance ◽

Soluble Fraction ◽

Limit Of Detection ◽

Curcuma Longa ◽

Correlation Coefficients ◽

Rf Values ◽

Aluminum Plates ◽

Hptlc Method

A simple, sensitive and precise high-performance thin-layer chromatographic (HPTLC) method was developed and validated for the analysis of ar-turmerone and turmerone, the major constituents of the lipid soluble fraction of the herbal medicament (HM) obtained from the rhizomes of Curcuma longa (turmeric). The separation was carried out on HPTLC aluminum plates precoated with silica gel 60F-254, with n-hexane:ethyl acetate (9.8:0.2 v/v) as mobile phase. Densitometric analysis of ar-turmerone and turmerone was carried out in the absorbance mode at 254 nm. This system was found to give compact spots for ar-turmerone and turmerone (Rf values 0.5 ± 0.05; 0.6 ± 0.04, respectively). A good linear regression relationship between peak areas and the concentrations was obtained over the range of 100–600 ng/spot, with correlation coefficients of 0.997 and 0.998 for ar-turmerone and turmerone, respectively. The limit of detection and quantification was found to be 20 and 40 ng/spot for ar-turmerone and turmerone, respectively. The method was further validated for precision and recovery. The RSD values of the precision were in the range 0.49–1.33% and spike recoveries were 99.9 and 100.0% for ar-turmerone and turmerone, respectively. Analysis of different batches of HM using the above method gave ar-turmerone and turmerone contents in the range of 25–30% and 30–38%. The developed HPTLC method can be applied for identification and quantitative determination of ar-turmerone and turmerone in the lipid soluble fractions of turmeric.

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Development of Validated Stability Indicating HPTLC Method for Assay of Ozagrel and its Pharmaceutical Formulations

International Journal of Scientific Research in Science Engineering and Technology ◽

10.32628/ijsrset11841111 ◽

2018 ◽

pp. 36-48 ◽

Cited By ~ 2

Author(s):

Vishal N Kushare ◽

Sachin S Kushare

Keyword(s):

High Performance ◽

Linear Regression Analysis ◽

Pharmaceutical Formulations ◽

Base Hydrolysis ◽

Assay Method ◽

Related Substance ◽

Stability Indicating ◽

Regular Production ◽

Hptlc Method

The present paper describes stability indicating high-performance thin-layer chromatography (HPTLC) assay method for Ozagrel in bulk drugs. The method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of toluene: methanol: triethylamine (6.5: 4.0: 0.1 v/v/v). The system was found to give compact spot for Ozagrel (Rf value of 0.40 ± 0.010). Densitometric analysis of Ozagrel was carried out in the absorbance mode at 280 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.999 with respect to peak area in the concentration range 30 - 120 ng/spot. The developed HPTLC method was validated with respect to accuracy, precision, recovery and robustness. Also to determine related substance and assay determination of Ozagrel that can be used to evaluate the quality of regular production samples. The developed method can also be conveniently used for the assay determination of Ozagrel in pharmaceutical formulations. The limits of detection and quantitation were 4.069 and 12.332 ng/spot, respectively by height. Ozagrel was subjected to acid and alkali hydrolysis, oxidation, photochemical and thermal degradation. The drug undergoes degradation under acidic, basic, oxidation and heat conditions. This indicates that the drug is susceptible to acid, base hydrolysis, oxidation and heat. Statistical analysis proves that the method is repeatable, selective and accurate for the estimation of said drug. The proposed developed HPTLC method can be applied for identification and quantitative determination of Ozagrel in bulk drug and tablet formulation.

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Validation and application of an HPTLC method for the determination of parthenolide in feverfew herbal products

JPC - Journal of Planar Chromatography - Modern TLC ◽

10.1556/jpc.17.2004.5.11 ◽

2004 ◽

Vol 17 (5) ◽

pp. 375-378 ◽

Cited By ~ 2

Author(s):

Ehab Abourashed

Keyword(s):

Herbal Products ◽

Hptlc Method

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QUANTITATIVE DETERMINATION OF CURCUMIN IN TURMERIC POWDER AND TURMERIC–HONEY BALL PILLS BY HPLC

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2018.4.6 ◽

2018 ◽

Vol 8 (4) ◽

pp. 42-47

Author(s):

Tien Nguyen Huu ◽

Tram Le Thi Bao ◽

Ngoc Nguyen Thi Nhu ◽

Thang Phan Phuoc ◽

Khan Nguyen Viet

Keyword(s):

Acetic Acid ◽

Gastric Mucosa ◽

Quantitative Determination ◽

Mobile Phase ◽

Curcuma Longa ◽

Biological Marker ◽

Hplc Method ◽

Chromatography Analysis ◽

Precision And Accuracy

Background: Curcumin is a major ingredient in turmeric (Curcuma longa L., Zingiberaceae), which has important activities such as anti-tumor, anti-inflammatory, antioxidant, anti-ischemia, protection of gastric mucosa etc,. Curcumin can be considered as a biological marker of turmeric and turmeric products. Objectives: Developing an HPLC method for quantification of curcumin in turmeric powder and turmeric - honey ball pills; applying this method for products on the market. Materials and methods: turmeric powder and turmeric - honey ball pills collected in Thua Thien Hue province. After optimization process, the method was validated and applied to evaluate the content of curcumin. Results: The chromatography analysis was performed with: Zorbaz Eclipse XDB-C18 (150 × 4.6 nm; 5 µm); Mobile phase: acetonitril: 2% acetic acid (45:55), Flow rate was kept constant at 1.0 ml/min; Detector PDA (420 nm). The method was validated for the HPLC system compatibility, specificity, linearity range, precision and accuracy; the recovery greater than 98%. Conclusion: The developed HPLC method can determine curcumin in turmeric powder and turmeric - honey ball pills. Key words: Curcumin, turmeric powder, turmeric-honey ball pills, quantitative determination, HPLC

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Determination of Glycaemic Responses of dairy yoghurt incorporated with spice oleoresins (Cinnamomum zeylanicum, Curcuma longa)

2020 From Innovation to Impact (FITI) ◽

10.1109/fiti52050.2020.9424883 ◽

2020 ◽

Author(s):

Dayani Pavalakumar ◽

Madhura Jayasinghe ◽

Maharsha Edirisinghe ◽

Isuru Wijesekara ◽

Subhashinie Senadheera

Keyword(s):

Curcuma Longa ◽

Cinnamomum Zeylanicum

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DETERMINATION OF 10-GINGEROL IN INDIAN GINGER BY VALIDATED HPTLC METHOD OF SAMPLES COLLECTED ACROSS SUBCONTINENT OF INDIA

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i12.14953 ◽

2016 ◽

Vol 8 (12) ◽

pp. 190

Author(s):

Kamran Ashraf ◽

Syed Adnan Ali Shah ◽

Mohd Mujeeb

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

High Performance ◽

Limit Of Detection ◽

Limit Of Quantification ◽

Chromatography Method ◽

Aluminum Plates ◽

Layer Chromatography ◽

Hptlc Method

Objective: A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as perICH guidelines.Methods: The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F254 using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm.Results: This system was found to have a compact spot of 10-gingerol at RF value of 0.57±0.03. For the proposed procedure, linearity (r2 = 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory.Conclusion: Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India.

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