Determination of Glycaemic Responses of dairy yoghurt incorporated with spice oleoresins (Cinnamomum zeylanicum, Curcuma longa)

2020 ◽  
Author(s):  
Dayani Pavalakumar ◽  
Madhura Jayasinghe ◽  
Maharsha Edirisinghe ◽  
Isuru Wijesekara ◽  
Subhashinie Senadheera
Keyword(s):  
Curcuma Longa ◽  
Cinnamomum Zeylanicum

QUANTITATIVE DETERMINATION OF CURCUMIN IN TURMERIC POWDER AND TURMERIC–HONEY BALL PILLS BY HPLC

2018 ◽  
Vol 8 (4) ◽  
pp. 42-47
Author(s):  
Tien Nguyen Huu ◽  
Tram Le Thi Bao ◽  
Ngoc Nguyen Thi Nhu ◽  
Thang Phan Phuoc ◽  
Khan Nguyen Viet
Keyword(s):  
Acetic Acid ◽  
Gastric Mucosa ◽  
Quantitative Determination ◽  
Mobile Phase ◽  
Curcuma Longa ◽  
Biological Marker ◽  
Hplc Method ◽  
Chromatography Analysis ◽  
Precision And Accuracy

Background: Curcumin is a major ingredient in turmeric (Curcuma longa L., Zingiberaceae), which has important activities such as anti-tumor, anti-inflammatory, antioxidant, anti-ischemia, protection of gastric mucosa etc,. Curcumin can be considered as a biological marker of turmeric and turmeric products. Objectives: Developing an HPLC method for quantification of curcumin in turmeric powder and turmeric - honey ball pills; applying this method for products on the market. Materials and methods: turmeric powder and turmeric - honey ball pills collected in Thua Thien Hue province. After optimization process, the method was validated and applied to evaluate the content of curcumin. Results: The chromatography analysis was performed with: Zorbaz Eclipse XDB-C18 (150 × 4.6 nm; 5 µm); Mobile phase: acetonitril: 2% acetic acid (45:55), Flow rate was kept constant at 1.0 ml/min; Detector PDA (420 nm). The method was validated for the HPLC system compatibility, specificity, linearity range, precision and accuracy; the recovery greater than 98%. Conclusion: The developed HPLC method can determine curcumin in turmeric powder and turmeric - honey ball pills. Key words: Curcumin, turmeric powder, turmeric-honey ball pills, quantitative determination, HPLC

scholarly journals MICROSCOPIC DIAGNOSTIC FEATURES OF WHOLE, MILLED AND POWDERED PLANT MATERIAL “RHIZOMES OF COMMON TURMERIC”

2016 ◽  
Vol 1 (1) ◽  
pp. 62-66
Author(s):  
VA A Kurkin ◽  
VM M Ryzhov ◽  
TK K Ryazanova ◽  
LV V Tarasenko ◽  
MYu Yu Borisov ◽  
...  
Keyword(s):  
Plant Material ◽  
Curcuma Longa ◽  
Powdered Material ◽  
Pigment Cells ◽  
Vascular Bundles ◽  
Diagnostic Features ◽  
Folded Structure ◽  
Pharmacopoeial Monograph ◽  
Narrow Lumen

Aim - the determination of microscopic diagnostic features of rhizomes of common turmeric (Curcuma longa L.), ginger family (Zingiberaceae). Materials and methods. We have studied whole, milled and powdered plant material of turmeric from different manufactures. The study was carried out with the use of the digital microscopes “Motic DM111” (Korea) and “Motic DM-39C-N9GO-A” (Korea). Results. It was determined that the microscopic diagnostic features of rhizomes of turmeric include the presence of cells with sinuous walls and structured content in the form of colored folding in the parenchyma; closed collateral vascular bundles lie scattered in the parenchyma, their conductive elements have narrow lumen. Vascular bundles contain pigment cells considerably smaller in comparison with the size of vessels. Pigment cells with drops of orange-red essential oil are also found in the parenchyma. In milled and powdered plant material, the fragments of all these elements are observed. In the fragments of powdered rhizomes of turmeric, numerous cells with yellow content are found; the parenchyma cells have less expressed folded structure. Fragments of vessels are found both in milled and powdered material. Conclusion. The results of this study were included in the section “Microscopy” of the project of pharmacopoeial monograph “Curcuma longa L. rhizomes”.

scholarly journals HPTLC: A Tool for Determination of Curcumin in Mammalian Samples

2017 ◽  
Vol 9 (4) ◽  
Author(s):  
Jigni Mishra ◽  
Ankit Tripathi ◽  
Kshipra Misra ◽  
Sarada S. K. S.
Keyword(s):  
Inflammatory Diseases ◽  
Curcuma Longa ◽  
Native Form ◽  
Tissue Samples ◽  
Mammalian Tissues ◽  
Bowel Diseases ◽  
Chloroform Methanol ◽  
Hptlc Method ◽  
Different Tissues

Curcumin, an important phytoconstituent obtained from Curcuma longa L. (turmeric) is used traditionally in the treatment of various inflammatory diseases like arthritis, stroke and bowel diseases, etc. Despite its many health benefits, instability of curcumin in plasma is a major issue. The retention of curcumin in plasma must be properly evaluated in order to establish its stability in biological systems. The current study presents an HPTLC method undertaken for detection of curcumin and determination of its stability in plasma and different tissues of rats. The plasma and tissue samples were appropriately processed to render them suitable for HPTLC analysis. The method employed HPTLC glass plates precoated with silica gel 60F254 as the stationary phase. The mobile phase developed consisted of chloroform, methanol and glacial acetic acid which successfully gave distinct bands for curcumin with a Rf value of 0.95. This newly developed HPTLC method was found to be reproducible and accurate in quantifying curcumin in mammalian samples. This method was further utilized to efficiently estimate the time for which curcumin is retained in its native form in mammalian tissues and plasma alike.

scholarly journals HPTLC Method for the Quantitative Determination of ar-Turmerone and Turmerone in Lipid Soluble Fraction from Curcuma longa

2007 ◽  
Vol 2 (9) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Vikas Jain ◽  
Vure Prasad ◽  
Satwayan Singh ◽  
Raghwendra Pal
Keyword(s):  
Quantitative Determination ◽  
High Performance ◽  
Soluble Fraction ◽  
Limit Of Detection ◽  
Curcuma Longa ◽  
Correlation Coefficients ◽  
Rf Values ◽  
Aluminum Plates ◽  
Hptlc Method

A simple, sensitive and precise high-performance thin-layer chromatographic (HPTLC) method was developed and validated for the analysis of ar-turmerone and turmerone, the major constituents of the lipid soluble fraction of the herbal medicament (HM) obtained from the rhizomes of Curcuma longa (turmeric). The separation was carried out on HPTLC aluminum plates precoated with silica gel 60F-254, with n-hexane:ethyl acetate (9.8:0.2 v/v) as mobile phase. Densitometric analysis of ar-turmerone and turmerone was carried out in the absorbance mode at 254 nm. This system was found to give compact spots for ar-turmerone and turmerone (Rf values 0.5 ± 0.05; 0.6 ± 0.04, respectively). A good linear regression relationship between peak areas and the concentrations was obtained over the range of 100–600 ng/spot, with correlation coefficients of 0.997 and 0.998 for ar-turmerone and turmerone, respectively. The limit of detection and quantification was found to be 20 and 40 ng/spot for ar-turmerone and turmerone, respectively. The method was further validated for precision and recovery. The RSD values of the precision were in the range 0.49–1.33% and spike recoveries were 99.9 and 100.0% for ar-turmerone and turmerone, respectively. Analysis of different batches of HM using the above method gave ar-turmerone and turmerone contents in the range of 25–30% and 30–38%. The developed HPTLC method can be applied for identification and quantitative determination of ar-turmerone and turmerone in the lipid soluble fractions of turmeric.

Improved HPTLC Method for Determination of Curcuminoids from Curcuma longa

2006 ◽  
Vol 29 (6) ◽  
pp. 877-887 ◽  
Author(s):  
Vijaylata Pathania ◽  
Ajai Prakash Gupta ◽  
Bikram Singh
Keyword(s):  
Curcuma Longa ◽  
Hptlc Method
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