Validation and application of an HPTLC method for the determination of parthenolide in feverfew herbal products

2004 ◽  
Vol 17 (5) ◽  
pp. 375-378 ◽  
Author(s):  
Ehab Abourashed
Keyword(s):  
Herbal Products ◽  
Hptlc Method

scholarly journals A HPTLC Method for Determination of Anethole in Essential Oil, Methanolic Extract of Foeniculum vulgare and Commercial Herbal Products

2020 ◽  
pp. 68-75 ◽  
Author(s):  
Prawez Alam ◽  
Mohammad H. Alqarni ◽  
Pravej Alam ◽  
Ahmed I. Foudah ◽  
Mohammed Ghazwani
Keyword(s):  
Essential Oil ◽  
Aromatic Plants ◽  
Foeniculum Vulgare ◽  
Herbal Products ◽  
Ich Guidelines ◽  
Oil Extract ◽  
Satisfactory Relationship ◽  
Herbal Formulations ◽  
Hptlc Method

A rapid and feasible method of HPTLC is standardized for quantification of anethole in essential oil’s extract and from herbal formulations of fennel seed. The developed densitometric HPTLC method was performed to estimate the existence of anethole in the essential oil, extract and herbal formulations of fennel with the optimized concentration of hexane: Ethyl acetate (8:2%, v/v, mobile phase) on glass coated silica gel 60 F254 plates (20 × 10 cm) scanned with the absorbance of λ260 nm under densitometric condition.  The Linearity of regressions revealed a satisfactory relationship between peak area and concentration of anethole in between the range of 100-600 ng/spot. This reliable method was validated as per the ICH guidelines to fulfill the necessary parameters such as accuracy and robustness. The amount of anethole in essential oil (0.098 ± 0.002%), extract (0.101 ± 0.004%) and three herbal formulations A (0.024 ± 0.004%), C (0.019 ± 0.002%) while anethole is not detected in B formulations from fennel seed was completely estimated by the developed method. The standardized methods and its validation gave new insights of HPTLC based detection and quantification of anethole in other aromatic plants as well as in other pharmacological formulations.

Development of Validated Stability Indicating HPTLC Method for Assay of Ozagrel and its Pharmaceutical Formulations

2018 ◽  
pp. 36-48 ◽  
Author(s):  
Vishal N Kushare ◽  
Sachin S Kushare
Keyword(s):  
High Performance ◽  
Linear Regression Analysis ◽  
Pharmaceutical Formulations ◽  
Base Hydrolysis ◽  
Assay Method ◽  
Related Substance ◽  
Stability Indicating ◽  
Regular Production ◽  
Hptlc Method

The present paper describes stability indicating high-performance thin-layer chromatography (HPTLC) assay method for Ozagrel in bulk drugs. The method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of toluene: methanol: triethylamine (6.5: 4.0: 0.1 v/v/v). The system was found to give compact spot for Ozagrel (Rf value of 0.40 ± 0.010). Densitometric analysis of Ozagrel was carried out in the absorbance mode at 280 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.999 with respect to peak area in the concentration range 30 - 120 ng/spot. The developed HPTLC method was validated with respect to accuracy, precision, recovery and robustness. Also to determine related substance and assay determination of Ozagrel that can be used to evaluate the quality of regular production samples. The developed method can also be conveniently used for the assay determination of Ozagrel in pharmaceutical formulations. The limits of detection and quantitation were 4.069 and 12.332 ng/spot, respectively by height. Ozagrel was subjected to acid and alkali hydrolysis, oxidation, photochemical and thermal degradation. The drug undergoes degradation under acidic, basic, oxidation and heat conditions. This indicates that the drug is susceptible to acid, base hydrolysis, oxidation and heat. Statistical analysis proves that the method is repeatable, selective and accurate for the estimation of said drug. The proposed developed HPTLC method can be applied for identification and quantitative determination of Ozagrel in bulk drug and tablet formulation.

scholarly journals DETERMINATION OF 10-GINGEROL IN INDIAN GINGER BY VALIDATED HPTLC METHOD OF SAMPLES COLLECTED ACROSS SUBCONTINENT OF INDIA

2016 ◽  
Vol 8 (12) ◽  
pp. 190
Author(s):  
Kamran Ashraf ◽  
Syed Adnan Ali Shah ◽  
Mohd Mujeeb
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Aluminum Plates ◽  
Layer Chromatography ◽  
Hptlc Method

<p><strong>Objective: </strong>A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as perICH guidelines.</p><p><strong>Methods: </strong>The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F<sub>254</sub> using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm.</p><p><strong>Results: </strong>This system was found to have a compact spot of 10-gingerol at <em>R</em><sub>F</sub> value of 0.57±0.03. For the proposed procedure, linearity (<em>r</em><sup>2</sup> = 0.998±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%–100.68%), were found to be satisfactory.</p><p><strong>Conclusion: </strong>Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India.</p>

New rapid validated HPTLC method for the determination of galanthamine in Amaryllidaceae plant extracts

2008 ◽  
Vol 19 (4) ◽  
pp. 353-358 ◽  
Author(s):  
Amina H. Abou‐Donia ◽  
Soad M. Toaima ◽  
Hala M. Hammoda ◽  
Eman Shawky
Keyword(s):  
Plant Extracts ◽  
Hptlc Method

RP-HPLC AND HPTLC STABILITY INDICATING ASSAY METHODS FOR IVERMECTIN IN BULK AND TABLET DOSAGE FORM

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (03) ◽  
pp. 32-42
Author(s):  
G. P Wani ◽  
◽  
S. B Jadhav
Keyword(s):  
Mobile Phase ◽  
Stress Testing ◽  
Hplc Method ◽  
Rp Hplc ◽  
Assay Methods ◽  
Photo Stability ◽  
Tablet Dosage ◽  
Hptlc Method ◽  
Alkaline Oxidation

Simple, rapid, precise, accurate RP-HPLC and HPTLC methods have been developed and validated for ivermectin in bulk and its marketed formulation. RP-HPLC method for drug was achieved on Grace C18 (250 mm X 4.6 ID, Particle size; 5 μ) column using mobile phase acetonitrile: 10 mM phosphate buffer (95:05 v/v) pH adjusted to 3 with o-phosphoric acid. Detection of drug was done at 245 nm. The retention time was found to be 5.83 min. HPTLC method for ivermectin was accomplished on a precoated silica gel aluminium plate 60F-254 (CAMAG Linomat 5), using toluene: methanol: glacial acetic acid (8:2:0.1 v/v/v) as a mobile phase. The densitometric scanning was performed at 245 nm which showed Rf 0.46 for ivermectin. The stress testing of the drug individually was carried out under acidic, alkaline, oxidation, photo-stability and thermal degradation conditions. The proposed methods were successfully applied for the determination of drug in bulk and its marketed formulation.

RP-HPLC AND HPTLC METHODS FOR THE ESTIMATION OF LUPEOL IN. HEMIDESMUS INDICUS LINN. ROOT EXTRACTS AND POLYHERBAL FORMULATION USING STANDARD MA

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (07) ◽  
pp. 40-46
Author(s):  
Jayaprakasam Rajendran ◽  
Anita Elizabeth Chacko ◽  
Tresa Thomas ◽  
Neethu Johnson ◽  
Kochupapy Ravi Thengungal
Keyword(s):  
Correlation Coefficient ◽  
Chemical Constituents ◽  
Hemidesmus Indicus ◽  
Polyherbal Formulation ◽  
Ich Guidelines ◽  
Soxhlet Apparatus ◽  
The Family ◽  
Herbal Plants ◽  
Hptlc Method

The roots of Hemidesmus indicus, belonging to the family Ascelpidaceae, are used as blood purifier, antileucorrhoeic, galactogenic, antidiarrhoeal, antirheumatic, antisyphilitic, febrifuge and possesses chemical constituents like triterpenoids (lupeol), flavonoids, glycosides and tannins. Roots were dried and extracted with petroleum ether, chloroform and methanol by using Soxhlet apparatus. Two simple and sensitive chromatographic methods, namely, HPTLC and HPLC, were developed for determination of lupeol from the extract of H. indicus and its polyherbal formulation and validated according to ICH guidelines. The HPTLC method linear regression data for the standard lupeol a concentration range of 400-900ng/spot and correlation coefficient (r) was 0.9904. The HPLC calibration curves of standard lupeol showed good linearity range from 20 to 100μg/ml and correlation coefficient (r) was 0.9929. The HPTLC and HPLC methods are simple, precise, accurate and specific. Hence, these methods can be used for the standardization of lupeol in herbal plants and polyherbal formulations.

scholarly journals Chromatographic Profiling of Rose Petals in Unani Formulations (Gulkand, Arq-e-Gulab, and Rose Sharbat) Using HPTLC and GC–MS

2020 ◽  
Vol 103 (3) ◽  
pp. 684-691
Author(s):  
Rabea Parveen ◽  
Sultan Zahiruddin ◽  
Akshay Charegaonkar ◽  
Abhijeet Khale ◽  
Saikat Mallick
Keyword(s):  
Traditional Medicine ◽  
Chromatographic Analysis ◽  
Herbal Products ◽  
Unani Medicine ◽  
Ms Analysis ◽  
Rose Petals ◽  
The Rose ◽  
Hptlc Method ◽  
Chromatographic Profiling

Abstract Background: One of the most precious systems of traditional medicine is the Unani system of medicine. A wide variety of formulations indigenous to the Unani medicine have been preserved over the years. These formulations are potent and effective even after ages of postformulation. Rose petals are an example of such an herb, which is extensively and popularly used in Unani formulations for edible and cosmetic purposes. Rose petals are rich in terpenes, glycosides, flavonoids, and anthocyanins. Objective: The aim of this study was to characterize the phytochemical profiling of different rose varieties and their marketed formulations. Method: HPTLC method was developed for detecting overall profile and assessing variations among the rose varieties available in market along with the popular formulations of rose such as gulkand (Brand A and Brand B), Arq-e-gulab or Gulab Jal (Brand C), and sharbat (Brand D). GC–MS analysis was also carried out for fingerprinting of rose varieties and formulations. Results: HPTLC and GC–MS fingerprinting showed some common peaks in rose samples as well as in the formulation samples. The methods also gave different peaks for the adulterant that might be used in place of rose. Conclusions: Both the methods could be used for standardization of herbal products containing rose as one of the ingredients and also used to check for the adulteration. Highlights: The current advanced chromatographic analysis is a valuable tool to determine the quality of the formulation.

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