scholarly journals High-performance liquid chromatography-diode array detector determination and availability of phenolic compounds in 10 genotypes of walnuts

2016 ◽  
Vol 20 (5) ◽  
pp. 1074-1084 ◽  
Author(s):  
Fernando Figueroa ◽  
Javier Marhuenda ◽  
Pilar Zafrilla ◽  
Débora Villaño ◽  
Adela Martínez-Cachá ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Performance ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector

scholarly journals Determination and Quantification of Gallic Acid in Raw Propolis by High-performance Liquid Chromatography–Diode Array Detector in Burundi

2019 ◽  
Vol 1 (1) ◽  
pp. 43-48
Author(s):  
Ramadhan Nyandwi ◽  
Ayşe S. Kılıç ◽  
Meltem Çelik ◽  
Hasan H. Oruç
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
High Performance ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Sub Saharan

Background: Honey, pollen, and propolis are among the products that bees process and derive from plants and flowers. Propolis is a resinous material that bees gather from the buds and bark of some trees and small plants. Propolis from temperate climates mainly contains phenolic compounds, in contrast with propolis from tropical climates, which mainly contains terpenes. This study aimed to determine, characterise, and quantify the phenolic content of raw propolis from Burundi. Methods: In this study, a total of 6 samples were collected from the provinces of Rumonge, Cibitoke, and Ruyigi in Burundi. Fifteen phenolic compounds (caffeic acid, ferulic acid, epigallocatechin gallate, isoferulic acid, cinnamic acid, caffeic acid phenethyl ester, gallic acid, apigenin, chrysin, galangin, quercetin, kaempherol, rutin trihydrate, naringenin, and pinocembrin) were used as high-performance liquid chromatography (HPLC) standards for qualitative and quantitative analyses of the propolis samples. Results: Among the 15 phenolic compounds checked, only 1 – gallic acid – was detected at a measurable level using an HPLC-diode array detector system. Conclusion: In addition to terpenes, propolis found in sub-Saharan Africa may contain phenolic compounds. Further advanced investigation of sub-Saharan African propolis is required for more detailed characterisation.

Determination and Quantification of Gallic Acid in Raw Propolis by High-performance Liquid Chromatography–Diode Array Detector in Burundi

2019 ◽  
Vol 1 (1) ◽  
pp. 43-48
Author(s):  
Ramadhan Nyandwi ◽  
Ayşe S. Kılıç ◽  
Meltem Çelik ◽  
Hasan H. Oruç
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
High Performance ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Sub Saharan

Background: Honey, pollen, and propolis are among the products that bees process and derive from plants and flowers. Propolis is a resinous material that bees gather from the buds and bark of some trees and small plants. Propolis from temperate climates mainly contains phenolic compounds, in contrast with propolis from tropical climates, which mainly contains terpenes. This study aimed to determine, characterise, and quantify the phenolic content of raw propolis from Burundi. Methods: In this study, a total of 6 samples were collected from the provinces of Rumonge, Cibitoke, and Ruyigi in Burundi. Fifteen phenolic compounds (caffeic acid, ferulic acid, epigallocatechin gallate, isoferulic acid, cinnamic acid, caffeic acid phenethyl ester, gallic acid, apigenin, chrysin, galangin, quercetin, kaempherol, rutin trihydrate, naringenin, and pinocembrin) were used as high-performance liquid chromatography (HPLC) standards for qualitative and quantitative analyses of the propolis samples. Results: Among the 15 phenolic compounds checked, only 1 – gallic acid – was detected at a measurable level using an HPLC-diode array detector system. Conclusion: In addition to terpenes, propolis found in sub-Saharan Africa may contain phenolic compounds. Further advanced investigation of sub-Saharan African propolis is required for more detailed characterisation.

scholarly journals Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of Tabernaemontana heyneana by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Thiyagarajan Sathishkumar ◽  
Ramakrishnan Baskar ◽  
Mohan Aravind ◽  
Suryanarayanan Tilak ◽  
Sri Deepthi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Shake Flask ◽  
Orthogonal Design ◽  
Array Detector ◽  
Diode Array ◽  
Ionization Mass ◽  
Diode Array Detector

Flavonoids are exploited as antioxidants, antimicrobial, antithrombogenic, antiviral, and antihypercholesterolemic agents. Normally, conventional extraction techniques like soxhlet or shake flask methods provide low yield of flavonoids with structural loss, and thereby, these techniques may be considered as inefficient. In this regard, an attempt was made to optimize the flavonoid extraction using orthogonal design of experiment and subsequent structural elucidation by high-performance liquid chromatography-diode array detector-electron spray ionization/mass spectrometry (HPLC-DAD-ESI/MS) techniques. The shake flask method of flavonoid extraction was observed to provide a yield of 1.2±0.13 (mg/g tissue). With the two different solvents, namely, ethanol and ethyl acetate, tried for the extraction optimization of flavonoid, ethanol (80.1 mg/g tissue) has been proved better than ethyl acetate (20.5 mg/g tissue). The optimal conditions of the extraction of flavonoid were found to be 85°C, 3 hours with a material ratio of 1 : 20, 75% ethanol, and 1 cycle of extraction. About seven different phenolics like robinin, quercetin, rutin, sinapoyl-hexoside, dicaffeic acid, and two unknown compounds were identified for the first time in the flowers of T. heyneana. The study has also concluded that L16 orthogonal design of experiment is an effective method for the extraction of flavonoid than the shake flask method.

Sign in / Sign up

Export Citation Format

Share Document