Knowledge-Based Approach to Control of Mixed Culture Cultivation for PHB Production Process

Abstract The paper proposes an approach using Genetic Algorithm (GA) for development of optimal time profiles of key control variable of Poly-HydroxyButyrate (PHB) production process. Previous work on modeling and simulation of PHB process showed that it is a highly nonlinear process that needs special controllers based on human experience, as such fuzzy logic controller proved to be a good choice. Fuzzy controllers are not totally replaced, due to the specific process knowledge that they contain. The achieved results are compared with previously proposed knowledge-based approach to the same optimal control task.

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Reaction Rate Estimators of Fed-Batch Process for Poly-β-Hydroxybutyrate (PHB) Production by Mixed Culture

Biotechnology & Biotechnological Equipment ◽

10.1080/13102818.2007.10817426 ◽

2007 ◽

Vol 21 (1) ◽

pp. 113-116 ◽

Cited By ~ 3

Author(s):

V. Lyubenova ◽

M. Ignatova ◽

M. Novak ◽

T. Patarinska

Keyword(s):

Mixed Culture ◽

Reaction Rate ◽

Batch Process ◽

Fed Batch ◽

Phb Production

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Enrichment of Methylocystis dominant mixed culture from rice field for PHB production

Journal of Biotechnology ◽

10.1016/j.jbiotec.2021.11.007 ◽

2021 ◽

Author(s):

Pranav P. Kulkarni ◽

Sambhaji B. Chavan ◽

Mandar S. Deshpande ◽

Dhanishta Sagotra ◽

Pramod S. Kumbhar ◽

...

Keyword(s):

Mixed Culture ◽

Rice Field ◽

Phb Production

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Production of poly-3-hydroxybutyrate from mixed culture

Biologia ◽

10.1515/biolog-2016-0097 ◽

2016 ◽

Vol 71 (7) ◽

Cited By ~ 2

Author(s):

Thomas Shalin ◽

Raveendran Sindhu ◽

Ashok Pandey ◽

Vincenza Faraco ◽

Parameswaran Binod

Keyword(s):

Mixed Culture ◽

Biodegradable Polymers ◽

Biodegradable Polymer ◽

The Other ◽

Cost Difference ◽

Microbial Cultures ◽

Phb Production ◽

Mixed Microbial Cultures ◽

Main Barrier ◽

The Cost

AbstractPoly-3-hydroxybutyrate (PHB) is a biodegradable polymer produced by many bacteria. Some of the properties are similar to thermoplastics like polypropylene, hence finding application PHB can directly replace non-biodegradable polymers. But the main barrier has been the cost difference. The utilization of mixed microbial cultures facilitates the use of complex substrates and thus can reduce the cost of PHB production. In the present study, mixed culture systems, where metabolite produced by one organism may be assimilated by the other organism, were employed.

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Growth of a Methane-Utilizing Mixed Culture HD6T on Methanol and Poly-β-Hydroxybutyrate Biosynthesis

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.160-162.171 ◽

2010 ◽

Vol 160-162 ◽

pp. 171-175 ◽

Cited By ~ 1

Author(s):

Jing Dong ◽

Jia Ying Xin ◽

Ying Xin Zhang ◽

Lin Lin Chen ◽

Hong Ye Liang ◽

...

Keyword(s):

Mixed Culture ◽

Mineral Salt Medium ◽

Biomass Concentration ◽

Dry Weight ◽

Maximum Growth ◽

Maximum Growth Rate ◽

Production Costs ◽

Lag Phase ◽

Phase Duration ◽

Phb Production

Methane-utilizing mixed culture HD6T was successfully cultivated in a brief non-sterile process using methanol as a sole carbon and energy source for the production of poly-β-hydroxybutyrate(PHB). Shake-flask experiments showed HD6T could grow well in the mineral salt medium with the addition of methanol exposed to the air directly. This non-sterile process and the use of cheap substrates (methanol) can reduce the production costs of PHB. It was found that HD6T grew better and PHB production in a more effective way with an initial liquid methanol concentration of 0.15%(v/v).The lag phase duration, the maximum growth rate, the biomass concentration and the PHB yield, for the optimal conditions were, respectively, 12.03h, 0.04h-1(OD600), 1.54g/l(dry weight), 0.424g/l(dry weight). Methane-utilizing mixed culture HD6T appears to be a promising organism for PHB production.

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Establishment of Fermentation Cultivation System to Produce PHB Using Methane-Utilizing Mixed Culture

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.200.389 ◽

2012 ◽

Vol 200 ◽

pp. 389-392

Author(s):

Jing Dong ◽

He Teng Wang

Keyword(s):

Mixed Culture ◽

High Cell Concentration ◽

Cultivation System ◽

Fermentation Method ◽

Phb Production ◽

Fermentation Tank ◽

Shake Flasks ◽

One Step ◽

Fermentation Strategy ◽

Good Productivity

A new fermentation strategy, one-step fermentation was developed for PHB production with high cell concentration and good productivity in a simplified way. Combined with the research results of PHB synthesis with the method of shake-flasks fermentation cultivation, the fermentation system was built based on the characteristics of fermentation tank cultivation,which was much superior to the traditional two-step fermentation. Based on shake flask studies, the full advantages of fermentation culture was taken to achieve the improvement of PHB production. Through this fermentation method with high PHB accumulation capacity, it provides a feasible way to expand the scale of PHB production, and promotes PHB industrial production process using Methane-Utilizing mixed Culture.

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