Establishment of Fermentation Cultivation System to Produce PHB Using Methane-Utilizing Mixed Culture

2012 ◽  
Vol 200 ◽  
pp. 389-392
Author(s):  
Jing Dong ◽  
He Teng Wang
Keyword(s):  
Mixed Culture ◽  
High Cell Concentration ◽  
Cultivation System ◽  
Fermentation Method ◽  
Phb Production ◽  
Fermentation Tank ◽  
Shake Flasks ◽  
One Step ◽  
Fermentation Strategy ◽  
Good Productivity

A new fermentation strategy, one-step fermentation was developed for PHB production with high cell concentration and good productivity in a simplified way. Combined with the research results of PHB synthesis with the method of shake-flasks fermentation cultivation, the fermentation system was built based on the characteristics of fermentation tank cultivation,which was much superior to the traditional two-step fermentation. Based on shake flask studies, the full advantages of fermentation culture was taken to achieve the improvement of PHB production. Through this fermentation method with high PHB accumulation capacity, it provides a feasible way to expand the scale of PHB production, and promotes PHB industrial production process using Methane-Utilizing mixed Culture.

scholarly journals review artikel study

2020 ◽  
Author(s):  
mulono apriyanto bin sugeng rijanto
Keyword(s):  
Mixed Culture ◽  
Fermentation Process ◽  
Improve Quality ◽  
Drying Method ◽  
Cocoa Beans ◽  
Sun Drying ◽  
Fermentation Method ◽  
Result Show ◽  
Traditional Process

Most cocoa beans in Indonesia are traditionally produced by farmers using non fermented and sun drying method. Thequality of cocoa beans produced by farmer in yogyakarta may be improved by the fermentation method. However, it needsoptimization for best fermentation process. The aims of the study was to improve quality of cocoa bans by fermentation ofsun dried cocoa beans. The characteric fermented cocoa beans was determined by maesuring changes pH, acidity andfermentation indexs of cocoa beans during fermentation. This study used preconditioned cocoa beans to resamble obtainfrom the farmer. Preconditioning was done in order to get 15% mouisture content of pulp at same level as moisture contentof pulp from traditional process. Before fermentation, therefor sun dried cocoa beans was rehydrated to obtain a moisturecontent of pulp similiar to fresh beans pulp, and then fermentation was conducted for 120 hours. Changes in acidity andfermentation indexs of cocoa beans during fermentation were measured. The fermentation process used 3 level treatmenti.e. control (without inoculum), mixed culture of microbies added at the begining fermentation. The result show all cocoabeans acidity increase during fermentation from 4,48% to 6,45% for control, 4,64% to 6,39% for addition of inoculum atbegining of fermentation and from 4,45% to 6,59% for addition of inoculum at the begining and midle of fermentation andfermentation indexs of cocoa beans increase for all level of inoculum addition i.e. 0,31 to 0,88 for control, 0,32 to 0,99 foraddition of inoculum at the beginning fermentation and 0,33 to 1,03 for addition of inoculum at the beginning and midle offermentation. The study indicated that addition of mix culture microbies in fermetation improved the quality of cocoabeans that characterized by pH, acidity and fermentation indexs of cocoa beans.

Mixed Culture Fermentations To Improve Nutritional Value of Corn Meal1

1988 ◽  
Vol 51 (11) ◽  
pp. 866-868
Author(s):  
M. L. FIELDS ◽  
A. AL-SHOSHAN ◽  
Y. POOSIRI
Keyword(s):  
Amino Acid ◽  
Mixed Culture ◽  
Nutritional Value ◽  
Nutritive Value ◽  
Mixed Cultures ◽  
Corn Meal ◽  
One Step ◽  
Significant Change ◽  
Amino Acid Balance ◽  
Relative Nutritive Value

One-step fermentation involving two microorganisms inoculated at the same time and two-step fermentations involving two inoculations of different microbes at different times were used to enrich corn meal. Starch in corn meal was first hydrolyzed by amylases of B. stearothermophilus, E. fibuligera or A. oryzae followed by the growth of C. utilis. The combination of E. fibuligera and C. utilis produced a significant (P<0.05) increase in lysine, methionine, tryptophan. The relative nutritive value (%), which reflected the amino acid balance, increased significantly (P<0.05) with this sequence of microorganisms. Niacin, riboflavin, and thiamin increased significantly (P<0.05) when mixed cultures of A. oryzae and E. fibuligera in combination with C. utilis were employed. When E. fibuligera alone was grown, no significant change was observed in thiamin content but significant increases occurred in niacin and riboflavin. A. oryzae by itself produced significant (P<0.05) changes in niacin, riboflavin and thiamin.

Biooxidation of a High-Grade Arsenopyritic Gold Ore Using a Mixed Culture of Moderate Thermophilic Microorganisms

2017 ◽  
Vol 262 ◽  
pp. 215-218
Author(s):  
Hoda Abdolahi ◽  
Ali Ahmadi ◽  
Hamid Zilouei ◽  
Maryam Khezri
Keyword(s):  
Mixed Culture ◽  
Pulp Density ◽  
Critical Parameters ◽  
High Grade ◽  
Stirred Tank Bioreactor ◽  
Thermophilic Microorganisms ◽  
Iron Extraction ◽  
Moderately Thermophilic ◽  
Gold Ore ◽  
Shake Flasks

This study investigated the biooxidation of a high-grade refractory gold ore from the Zarshouran mine (West Azarbaijan, Iran) in shake flasks and a stirred tank bioreactor (STBR) using a mixed culture of moderately thermophilic microorganisms. The influence of four critical parameters including, pH, biooxidation time, nutrient medium type and pulp density on the iron and arsenic extraction as well as gold cyanidation were evaluated in a full factorial design in shake flasks at 45 °C. Maximum iron extraction was obtained in M9K medium, pulp density of 5% (w/v), 15 days of biooxidation time and the pH of 1.6. Biooxidation in the STBR was carried out at the pulp densities of 10% and 20% (w/v), the pH of 1.7 in the M9K medium, in which dissolved oxygen, pH, redox potential, iron and arsenic concentrations were measured during the process. It was found that 61% decrement of sulphur content in the STBR led to recover 80% of gold, which was 43% higher than that in the conventional cyanidation process. It can be concluded that the oxidation of refractory arsenopyrite gold ore can achieved using a moderately thermophilic biooxidation culture.

scholarly journals Cultivo do Tomate Cereja sob Sistema Hidropônico: Influência do Turno de Rega

UNICIÊNCIAS ◽  
2018 ◽  
Vol 22 (1) ◽  
pp. 20
Author(s):  
Diogo Chamberlain Gonçalves ◽  
Carlos Henrique Dos Santos Fernandes ◽  
Débora Perdigão Tejo ◽  
Thaís Cristina Morais Vidal
Keyword(s):  
Solanum Lycopersicum ◽  
Cherry Tomato ◽  
Tomato Crop ◽  
Cultivation System ◽  
Advantages And Disadvantages ◽  
Hydroponic Cultivation ◽  
Crop Cycle ◽  
Hydroponic Systems ◽  
Good Productivity ◽  
Cherry Tomatoes

O cultivo hidropônico em tomate cereja é uma técnica que vem cada vez mais se expandindo no país. São necessárias várias metodologias para que se obtenham bons resultados de produtividade. O objetivo do presente trabalho foi conhecer e debater através de revisão bibliográfica como o turno de rega pode ser utilizado como ferramenta para melhorias de sistemas hidropônicos, especificamente, para cultura do tomate cereja, relatando as vantagens e desvantagens que o sistema hidropônico pode agregar comparado ao sistema de cultivo convencional, além de abordar aspectos referentes à cultura do tomate cereja, como: exigências climáticas e comercialização. A solução nutritiva adotada em sistemas de hidroponia  considera os elementos que são necessários para o desenvolvimento da planta, vale ressaltar que o tomateiro é uma planta altamente exigente em nutrientes. O turno de rega pode ser empregado para redução de gastos com energia elétrica, menor utilização de nutrientes, bem como diminuir a quantidade de água utilizada para circulação no sistema. A hidroponia se apresenta como técnica de cultivo muito utilizada para diversas culturas, amenizando os problemas fitossanitários, melhorando o controle dos aspectos nutricionais, redução do ciclo da cultura e obtenção de olerícolas de alta qualidade com alto padrão comercial. A adequação do turno de rega, em tomate cereja, é economicamente viável e sustentável ao produtor, diminuindo gastos e otimizando a produção. Palavras-chave: Solanum lycopersicum var. cerasiforme. Tomate. Hidroponia.AbstractThe hydroponic cultivation in cherry tomatoes is a technique that has been increasingly expanding in the country. Several methodologies are required to achieve good productivity results. The purpose of the present work was to know and debate through a bibliographical review how the irrigation shift can be used as a tool for improvements of hydroponic systems, specifically for cherry tomato crop, reporting the advantages and disadvantages that the hydroponic system can add compared to the conventional cultivation system, besides addressing aspects related to the cherry tomato crop as climatic requirements and commercialization. The nutrient solution, adopted in hydroponic systems considers the elements that are necessary for the plant development, it is worth mentioning that   tomato is a plant highly demanding in nutrients. The irrigation shift can be used to reduce expenses with electricity, less use of nutrients as well as decrease the amount of water used for circulation in the system. Hydroponics is presented  as a widely used cropping technique for many crops, mitigating phytosanitary problems, improving the control of nutritional aspects, reducing the crop cycle and obtaining high quality olericultural plants with high commercial standards. The suitability of the irrigation shift in cherry tomatoes is economically viable and sustainable to the producer, reducing expenses and optimizing production.Keywords: Solanum lycopersicum var. cerasiforme. Tomato. Hydroponics

Enrichment of Methylocystis dominant mixed culture from rice field for PHB production

2021 ◽  
Author(s):  
Pranav P. Kulkarni ◽  
Sambhaji B. Chavan ◽  
Mandar S. Deshpande ◽  
Dhanishta Sagotra ◽  
Pramod S. Kumbhar ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Rice Field ◽  
Phb Production

Production of poly-3-hydroxybutyrate from mixed culture

Biologia ◽  
2016 ◽  
Vol 71 (7) ◽  
Author(s):  
Thomas Shalin ◽  
Raveendran Sindhu ◽  
Ashok Pandey ◽  
Vincenza Faraco ◽  
Parameswaran Binod
Keyword(s):  
Mixed Culture ◽  
Biodegradable Polymers ◽  
Biodegradable Polymer ◽  
The Other ◽  
Cost Difference ◽  
Microbial Cultures ◽  
Phb Production ◽  
Mixed Microbial Cultures ◽  
Main Barrier ◽  
The Cost

AbstractPoly-3-hydroxybutyrate (PHB) is a biodegradable polymer produced by many bacteria. Some of the properties are similar to thermoplastics like polypropylene, hence finding application PHB can directly replace non-biodegradable polymers. But the main barrier has been the cost difference. The utilization of mixed microbial cultures facilitates the use of complex substrates and thus can reduce the cost of PHB production. In the present study, mixed culture systems, where metabolite produced by one organism may be assimilated by the other organism, were employed.

Growth of a Methane-Utilizing Mixed Culture HD6T on Methanol and Poly-β-Hydroxybutyrate Biosynthesis

2010 ◽  
Vol 160-162 ◽  
pp. 171-175 ◽  
Author(s):  
Jing Dong ◽  
Jia Ying Xin ◽  
Ying Xin Zhang ◽  
Lin Lin Chen ◽  
Hong Ye Liang ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Mineral Salt Medium ◽  
Biomass Concentration ◽  
Dry Weight ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Production Costs ◽  
Lag Phase ◽  
Phase Duration ◽  
Phb Production

Methane-utilizing mixed culture HD6T was successfully cultivated in a brief non-sterile process using methanol as a sole carbon and energy source for the production of poly-β-hydroxybutyrate(PHB). Shake-flask experiments showed HD6T could grow well in the mineral salt medium with the addition of methanol exposed to the air directly. This non-sterile process and the use of cheap substrates (methanol) can reduce the production costs of PHB. It was found that HD6T grew better and PHB production in a more effective way with an initial liquid methanol concentration of 0.15%(v/v).The lag phase duration, the maximum growth rate, the biomass concentration and the PHB yield, for the optimal conditions were, respectively, 12.03h, 0.04h-1(OD600), 1.54g/l(dry weight), 0.424g/l(dry weight). Methane-utilizing mixed culture HD6T appears to be a promising organism for PHB production.

scholarly journals Applications of one-Step environmentally-friendly fermentation method to produce fumaric acid with immobilized Rhizopus arrhizus in stirred-tank reactor

BioResources ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. 8197-8204
Author(s):  
Yuchen Ning ◽  
Yang Li ◽  
Shijie Zhao ◽  
Huan Liu ◽  
Li Deng ◽  
...  
Keyword(s):  
Fumaric Acid ◽  
Fermentation Process ◽  
Stirred Tank ◽  
Production Cycle ◽  
Total Production ◽  
Stirred Tank Reactor ◽  
Tank Reactor ◽  
Fermentation Method ◽  
One Step ◽  
The One

The traditional two-step cultivation mode for fungal cultivation is commonly divided into the seed stage (usually 24 to 48 h) and the production stage (usually 96 to 144 h). The use of two stages prolongs the total production cycle and generates excess wastewater. In this work, an efficient and environmentally friendly one-step fermentation method was applied for the production of fumaric acid by immobilized Rhizopus arrhizus in a stirred-tank reactor. The nitrogen source content and the agitation speed of the reactor were optimized as the two critical factors in the one-step fermentation process; the fumaric acid production of 51 g/L with a yield of 0.42 g/g glucose was comparable with the product from the traditional two-step fermentation process. Furthermore, the total production cycle was shortened to 96 h, and the amount of wastewater was reduced due to the avoidance of the seed culture process. Thus, utilization of the one-step fermentation method to produce fumaric acid was shown to be preferable feasibility and environmental-friendliness. This is a promising method for industrial production of fumaric acid and other high-value biochemicals by fungus.

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