In vitro antibacterial activity and in vivo therapeutic effect of Sesbania grandiflora in bacterial infected silkworms

Abstract Background The genus Burkholderia contains several pathogenic species with distinct etiologies, including Burkholderia pseudomallei the biothreat pathogen responsible for melioidosis and Burkholderia mallei which causes glanders. β-Lactams, such as ceftazidime and meropenem, are important therapeutic options for these infections. However, clinical resistance to β-lactams, which is primarily mediated by multiple types of β-lactamases in these species, is a growing concern. Durlobactam (ETX2514, DUR) is a novel β-lactamase inhibitor with broad-spectrum activity against Ambler class A, C, and D β-lactamases. Sulbactam (SUL) is an Ambler Class A β-lactamase inhibitor with intrinsic antibacterial activity against a limited number of species, including Acinetobacter spp. SUL-DUR is currently in Phase 3 clinical testing for the treatment of carbapenem-resistant infections caused by Acinetobacter spp. In this study, SUL-DUR was tested for in vitro antibacterial activity against B. pseudomallei and B. mallei as well as for in vivo efficacy in a preclinical model of melioidosis. Methods The antibacterial activity of SUL alone or in combination with DUR (fixed at 4 mg/L) against B. pseudomallei (n = 30) and B. mallei (N = 28) was determined following CLSI guidelines. In vivo efficacy was tested in an acute murine model of melioidosis in which 4 × 104 cfu Bp K96423 (SUL-DUR MIC = 1 mg/L) was administered intranasally to BalbC mice. SUL-DUR (100/200 or 400/200 mg/kg) was administered q4h subcutaneously 4 hours post-challenge for 6 days and murine survival was monitored for 45 days. Doxycycline (DOX) and ciprofloxacin (CIP) were dosed as positive controls at 40 mg/kg q12 h for 6 days. Results The addition of DUR effectively lowered the SUL MIC50/90 from 8/16 to 0.25/0.5 mg/L vs. B. pseudomallei and from 8/8 to 1/2 mg/L for B. mallei. All untreated mice in the melioidosis model succumbed to infection within 3 days of challenge. 60% survival was observed for both dose arms of SUL-DUR as compared with 40% survival observed for both CIP and DOX. Conclusion Preliminary preclinical data demonstrating robust in vitro and in vivo antibacterial activity of SUL-DUR against Burkholderia spp. suggests this combination may be an effective new therapy for the treatment of these challenging pathogens. Disclosures All authors: No reported disclosures.

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In vitro Antibacterial Activity and In vivo Acute Toxicological Studies of Nelsonia campestris Aqueous Leaf Exrtact

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2015/16081 ◽

2015 ◽

Vol 7 (1) ◽

pp. 27-35 ◽

Cited By ~ 2

Author(s):

H. Muhammad ◽

H. Makun ◽

A. Kabiru ◽

A. Mann ◽

M. Busari ◽

...

Keyword(s):

Antibacterial Activity ◽

Toxicological Studies ◽

In Vitro Antibacterial Activity

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Synergic fabrication of titanium dioxide incorporation into heparin-polyvinyl alcohol nanocomposite: Enhanced in vitro antibacterial activity and care of in vivo burn injury

Materials Research Express ◽

10.1088/2053-1591/abe1fb ◽

2021 ◽

Author(s):

Shougen Li ◽

Jia Zeng ◽

Dongfang Yin ◽

Peiqiang Liao ◽

Siqing Ding ◽

...

Keyword(s):

Antibacterial Activity ◽

Titanium Dioxide ◽

Polyvinyl Alcohol ◽

Burn Injury ◽

In Vitro Antibacterial Activity

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IN VITRO ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF SEEDPOD AND QUERCETIN OF NELUMBO NUCIFERA GAERTN

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i1.28915 ◽

2019 ◽

Vol 12 (1) ◽

pp. 164

Author(s):

Ruvanthika Pn ◽

Manikandan S

Keyword(s):

Antibacterial Activity ◽

Ethanolic Extract ◽

Antibacterial Activities ◽

Nelumbo Nucifera ◽

Diffusion Method ◽

Dependent Manner ◽

E Coli ◽

In Vitro Antibacterial Activity

Objective: The objective of the study was to evaluate whether ethanolic extracts of Nelumbo nucifera (EENN) seedpod and quercetin (active component of NN) possess antibacterial proprieties against Gram (-) bacteria such as Escherichia coli and Pseudomonas aeruginosa and Gram (+) bacteria such as Staphylococcus aureus. Methods: Antibacterial activities of EENN seedpod and quercetin were investigated using disc diffusion method, minimum inhibitory concentration against E. coli and P. aeruginosa and Gram (+) bacteria such as S. aureus. Results: The antibacterial activity of both EENN seedpod and quercetin was found to be increased in dose-dependent manner. The maximum zone of inhibition was exhibited by both EENN seedpod and quercetin against E. coli (14 mm and 15 mm) and P. aeruginosa (13 mm and 15 mm). Gram-negative bacteria were more susceptible to the EENN seedpod extract and quercetin than Gram-positive bacteria.Conclusion: The results of the present study suggested that the effect of EENN seedpod and quercetin against the tested bacteria in vitro may contribute to the in vivo activities of the EENN seedpod and quercetin.

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Plant essential oils against bacteria isolated from fish: an in vitro screening and in vivo efficacy of Lippia origanoides

Ciência Rural ◽

10.1590/0103-8478cr20190064 ◽

2019 ◽

Vol 49 (6) ◽

Author(s):

Guerino Bandeira Junior ◽

Carine de Freitas Souza ◽

Matheus Dellaméa Baldissera ◽

Sharine Nunes Descovi ◽

Bibiana Petri da Silveira ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Bacterial Infections ◽

Cupressus Sempervirens ◽

Therapeutic Success ◽

Plant Essential Oils ◽

In Vitro Antibacterial Activity ◽

Fish Bacteria

ABSTRACT: The use of natural products, such as essential oils (EOs), is a potential novel approach to treat fish bacterial infections with a lower risk of developing resistance. There has been a number of studies reporting the activity of EOs as those obtained from the species Achyrocline satureioides, Aniba parviflora, Aniba rosaeodora, Anthemis nobilis, Conobea scoparioides, Cupressus sempervirens, Illicium verum, Lippia origanoides, and Melaleuca alternifolia against bacteria. However, there are few studies investigating the effect of these EOs against fish bacteria. Therefore, the aim of this study was to evaluate the in vitro antibacterial activity of EOs against the following fish bacteria, Aeromonas hydrophila, Citrobacter freundii, and Raoultella ornithinolytica. Additionally, the in vivo antibacterial activity of the EO L. origanoides was evaluated against experimentally induced A. hydrophila infection of silver catfish (Rhamdia quelen). The EO of L. origanoides was chosen as it showed the highest in vitro antibacterial activity, with minimum inhibitory concentrations ranging from 0.2 to 0.8 mg mL-1. This EO also presented a therapeutic success of 58.33%, on a 30 day A. hydrophila infection. Therefore, we suggested that the EO of L. origanoides may be a viable alternative as a treatment for A. hydrophila infection.

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