scholarly journals Future potential of metagenomics in clinical laboratories

2021 ◽  
Author(s):  
Leonard Schuele ◽  
Hayley Cassidy ◽  
Nilay Peker ◽  
John W.A. Rossen ◽  
Natacha Couto
Keyword(s):  
Clinical Laboratories ◽  
Future Potential

Utilizing Dark Field Electron Microscopy to Produce Lantern Slides

1974 ◽  
Vol 32 ◽  
pp. 116-117
Author(s):  
J. N. Meador ◽  
C. N. Sun ◽  
H. J. White
Keyword(s):  
Electron Microscope ◽  
Electron Micrographs ◽  
Dark Field ◽  
Limiting Factor ◽  
Clinical Laboratories ◽  
Field Electron ◽  
Time Element ◽  
Field Electron Microscopy ◽  
Dark Field Electron ◽  
Dark Field Micrographs

The electron microscope is being utilized more and more in clinical laboratories for pathologic diagnosis. One of the major problems in the utilization of the electron microscope for diagnostic purposes is the time element involved. Recent experimentation with rapid embedding has shown that this long phase of the process can be greatly shortened. In rush cases the making of projection slides can be eliminated by taking dark field electron micrographs which show up as a positive ready for use. The major limiting factor for use of dark field micrographs is resolution. However, for conference purposes electron micrographs are usually taken at 2.500X to 8.000X. At these low magnifications the resolution obtained is quite acceptable.

Imprecision of Laboratory Determinations and Diagnostic Accuracy: Theoretical Considerations

1974 ◽  
Vol 13 (03) ◽  
pp. 151-158 ◽  
Author(s):  
D. A. B. Lindbebo ◽  
Fr. R. Watson
Keyword(s):  
Mathematical Model ◽  
Diagnostic Accuracy ◽  
Diagnostic Process ◽  
Clinical Laboratories ◽  
Non Linear ◽  
Theoretical Considerations ◽  
Made In

Recent studies suggest the determinations of clinical laboratories must be made more precise than at present. This paper presents a means of examining benefits of improvement in precision. To do this we use a mathematical model of the effect upon the diagnostic process of imprecision in measurements and the influence upon these two of Importance of Diagnosis and Prevalence of Disease. The interaction of these effects is grossly non-linear. There is therefore no proper intuitive answer to questions involving these matters. The effects can always, however, be calculated.Including a great many assumptions the modeling suggests that improvements in precision of any determination ought probably to be made in hospital rather than screening laboratories, unless Importance of Diagnosis is extremely high.

Factor VII Clotting Assay: Influence of Different Thromboplastins and Factor VII-Deficient Plasmas

1991 ◽  
Vol 65 (02) ◽  
pp. 160-164 ◽  
Author(s):  
Marina Poggio ◽  
Armando Tripodi ◽  
Guglielmo Mariani ◽  
Pier Mannuccio Mannucci ◽  
Keyword(s):  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Dose Response ◽  
Response Curve ◽  
Ischemic Heart ◽  
Factor Vii ◽  
Response Curves ◽  
Assay Sensitivity ◽  
Clinical Laboratories ◽  
Coagulant Activity

SummaryBeing a putative predictor of ischemic heart disease, the measurement of factor VII (FVTI) coagulant activity will be presumably requested to clinical laboratories with increasing frequency. To assess the influence on FVII assays of different thromboplastins and FVII-deficient plasmas we compared performances of all possible combinations of 5 thromboplastins and 6 deficient plasmas. The reproducibility of the clotting times of the dose-response curves for human and rabbit thromboplastins were acceptable (CV lower than 7%), whereas bovine thromboplastin had a higher CV. Reproducibility was very similar for all deficient plasmas when they were used in combination with a given thromboplastin. Responsiveness of the dose-response curve did not depend on the deficient plasma but rather on the thromboplastin: one rabbit thromboplastin was the least responsive, the bovine thromboplastin the most responsive, the human and the remaining two rabbit thromboplastins had intermediate responsiveness. Assay sensitivity to cold-activated FVII varied according to the thromboplastin: the bovine thromboplastin was the most sensitive, the human thromboplastin the least sensitive, of the three rabbit thromboplastins two were relatively sensitive, one was almost insensitive. In conclusion, our results indicate that thromboplastin rather than deficient plasma is the crucial factor in the standardization of FVII assay.

Studi Taksonomi dan Potensi Beberapa Jamur Liar di Pulau Belitong

2020 ◽  
Vol 3 (1) ◽  
pp. 24
Author(s):  
Ivan Permana Putra
Keyword(s):  
Bioactive Compounds ◽  
Schizophyllum Commune ◽  
Ecological Role ◽  
Future Potential

Abstract : Macrofungi or mushroom are cosmopolitan heterotrophic organisms which have a very important ecological role. Information on the presence and description of macrofungi can be used as a reference for conservation steps and future potential utilization. The purpose of this study was to provide  description of macro fungi and their potential utilization at Belitong Island. All macro fungi found were Basidiomycota. The identification results showd there were 11 species of mushrooms, i.e. Chlorophyllum molybdites, Lepiota sp., Marasmius sp., Parasola sp., Phallus indusiatus, Lentinus sp.1, Lentinus sp.2, Microporus sp., Polyporus cf. tricholoma, Russula sp., dan Schizophyllum commune. All mushrooms are decomposer at the study site. Some mushrooms have potential as food, medicine and source of other bioactive compounds. This paper explains how to use macroscopic characters to help the identification of macrofungi.Abstrak : Jamur makro merupakan organisme heterotof kosmopolitan yang memiliki peran ekologis yang sangat penting. Informasi mengenai keberadaan dan deskripsi jamur makro dapat dijadikan acuan untuk langkah konservasi serta pemanfaatan potensinya di masa mendatang. Tujuan dari penelitian ini adalah untuk menyediakan deskripsi jamur makro dan potensi pemanfaatannya di Pulau Belitong. Seluruh jamur makro yang ditemukan merupakan Filum Basidiomycota. Hasil identifikasi menunjukan terdapat 11 spesies jamur yaitu Chlorophyllum molybdites, Lepiota sp., Marasmius sp., Parasola sp., Phallus indusiatus, Lentinus sp.1, Lentinus sp.2, Microporus sp., Polyporus cf. tricholoma, Russula sp., dan Schizophyllum commune. Seluruh jamur merupakan dekomposer pada lokasi penelitian. Beberapa jamur berpotensi sebagai bahan pangan, obat, dan sumber senyawa bioaktif lainnya. Pada tulisan ini dijelaskan cara menggunakan karakter makroskopik untuk membantu identifikasi jamur.

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