In vivo effects of 1,4-dioxane on genotoxic parameters and behavioral alterations in Drosophila melanogaster

2022 ◽  
pp. 1-17
Author(s):  
Fatma Turna Demir
Keyword(s):  
Drosophila Melanogaster ◽  
Behavioral Alterations ◽  
In Vivo Effects

scholarly journals In Vivo Effects Of Traditional Ayurvedic Formulations in Drosophila melanogaster Model Relate with Therapeutic Applications

PLoS ONE ◽  
2012 ◽  
Vol 7 (5) ◽  
pp. e37113 ◽  
Author(s):  
Vibha Dwivedi ◽  
E. M. Anandan ◽  
Rajesh S. Mony ◽  
T. S. Muraleedharan ◽  
M. S. Valiathan ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Therapeutic Applications ◽  
In Vivo Effects

scholarly journals Short Aβ peptides attenuate Aβ42 toxicity in vivo

2017 ◽  
Vol 215 (1) ◽  
pp. 283-301 ◽  
Author(s):  
Brenda D. Moore ◽  
Jason Martin ◽  
Lorena de Mena ◽  
Jonatan Sanchez ◽  
Pedro E. Cruz ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Relative Abundance ◽  
Amyloid Β ◽  
Behavioral Alterations ◽  
Adeno Associated Virus ◽  
Aβ Peptides ◽  
Dose Dependent Fashion ◽  
Multiple Species ◽  
Dose Dependent

Processing of amyloid-β (Aβ) precursor protein (APP) by γ-secretase produces multiple species of Aβ: Aβ40, short Aβ peptides (Aβ37–39), and longer Aβ peptides (Aβ42–43). γ-Secretase modulators, a class of Alzheimer’s disease therapeutics, reduce production of the pathogenic Aβ42 but increase the relative abundance of short Aβ peptides. To evaluate the pathological relevance of these peptides, we expressed Aβ36–40 and Aβ42–43 in Drosophila melanogaster to evaluate inherent toxicity and potential modulatory effects on Aβ42 toxicity. In contrast to Aβ42, the short Aβ peptides were not toxic and, when coexpressed with Aβ42, were protective in a dose-dependent fashion. In parallel, we explored the effects of recombinant adeno-associated virus–mediated expression of Aβ38 and Aβ40 in mice. When expressed in nontransgenic mice at levels sufficient to drive Aβ42 deposition, Aβ38 and Aβ40 did not deposit or cause behavioral alterations. These studies indicate that treatments that lower Aβ42 by raising the levels of short Aβ peptides could attenuate the toxic effects of Aβ42.

Insights into amyloid disease from fly models

2014 ◽  
Vol 56 ◽  
pp. 69-83 ◽  
Author(s):  
Ko-Fan Chen ◽  
Damian C. Crowther
Keyword(s):  
Drosophila Melanogaster ◽  
Neurodegenerative Disorders ◽  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
Disease Pathogenesis ◽  
Amyloid Aggregates ◽  
Aβ Amyloid ◽  
Polypeptide Chains ◽  
Amyloid Diseases

The formation of amyloid aggregates is a feature of most, if not all, polypeptide chains. In vivo modelling of this process has been undertaken in the fruitfly Drosophila melanogaster with remarkable success. Models of both neurological and systemic amyloid diseases have been generated and have informed our understanding of disease pathogenesis in two main ways. First, the toxic amyloid species have been at least partially characterized, for example in the case of the Aβ (amyloid β-peptide) associated with Alzheimer's disease. Secondly, the genetic underpinning of model disease-linked phenotypes has been characterized for a number of neurodegenerative disorders. The current challenge is to integrate our understanding of disease-linked processes in the fly with our growing knowledge of human disease, for the benefit of patients.

In vivo effects of TGF-β1 in lung surfactant regulation, lung meachanics amd structure

Pneumologie ◽  
2014 ◽  
Vol 68 (06) ◽  
Author(s):  
E Lopez-Rodriguez ◽  
C Boden ◽  
S Knippenberg ◽  
A Pascual ◽  
J Perez-Gil ◽  
...  
Keyword(s):  
Lung Surfactant ◽  
In Vivo Effects ◽  
Tgf Β1

Evaluation of Sacroiliac Wedge Rotation to Increase Acetabular Ventroversion

1999 ◽  
Vol 12 (04) ◽  
pp. 173-177 ◽  
Author(s):  
R. L. Aper ◽  
M. D. Brown ◽  
M. G. Conzemius
Keyword(s):  
Hip Joint ◽  
Hip Dysplasia ◽  
Clinical Effect ◽  
Pelvic Osteotomy ◽  
Alternative Method ◽  
Angular Measurements ◽  
Si Joint ◽  
In Vivo Effects ◽  
Canine Hip Dysplasia

SummaryTreatment of canine hip dysplasia (CHD) via triple pelvic osteotomy (TPO) is widely accepted as the treatment that best preserves the existing hip joint. TPO, however, has several important disadvantages. In an effort to avoid some of the difficulties associated with TPO an alternative method of creating acetabular ventroversion (AW) was sought. The purpose of this study was to explore the effects of placement of a wedge in the sacroiliac (SI) joint on A W and to compare this to the effect of TPO on A W . On one hemipelvis a 30° pelvic osteotomy plate was used for TPO. The contralateral hemipelvis had a 28° SI wedge inserted into the SI joint. Pre- and postsurgical radiographs of each pelvis were taken and the angular measurements were recorded. On average, the 28° SI wedge resulted in 20.9° of A W, the 30° canine pelvic osteotomy plate resulted in 24.9° A W . Significant differences were not found (p >0.05) between the two techniques. Sacroiliac wedge rotation effectively creates A W and has several theoretical advantages when compared to TPO. The in vivo effects of sacroiliac wedge rotation should be studied in order to evaluate the clinical effect of the technique.Sacroiliac wedge rotation was tested as an alternative method to increase the angle of acetabular ventroversion. This technique effectively rotated the acetabulum and has several theoretical advantages when compared to triple pelvic osteotomy.

Dichloroacetate--its in vivo effects on carbohydrate metabolism in the conscious dog

Diabetes ◽  
1980 ◽  
Vol 29 (9) ◽  
pp. 702-709 ◽  
Author(s):  
M. P. Diamond ◽  
R. C. Rollings ◽  
L. Erlendson ◽  
P. E. Williams ◽  
W. W. Lacy ◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Conscious Dog ◽  
In Vivo Effects

Evaluation of the In Vivo Acute Toxicity and In Vitro Hemolytic and Immunomodulatory Activities of the Moringa oleifera Flower Trypsin Inhibitor (MoFTI)

2020 ◽  
Vol 27 ◽  
Author(s):  
Leydianne Leite de Siqueira Patriota ◽  
Dayane Kelly Dias do Nascimento Santos ◽  
Bárbara Rafaela da Silva Barros ◽  
Lethícia Maria de Souza Aguiar ◽  
Yasmym Araújo Silva ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Trypsin Inhibitor ◽  
Hemolytic Activity ◽  
Moringa Oleifera ◽  
Biological Activities ◽  
Hematological Parameters ◽  
Behavioral Alterations ◽  
Female Mice

Background: Protease inhibitors have been isolated from plants and present several biological activities, including immunomod-ulatory action. Objective: This work aimed to evaluate a Moringa oleifera flower trypsin inhibitor (MoFTI) for acute toxicity in mice, hemolytic activity on mice erythrocytes and immunomodulatory effects on mice splenocytes. Methods: The acute toxicity was evaluated using Swiss female mice that received a single dose of the vehicle control or MoFTI (300 mg/kg, i.p.). Behavioral alterations were observed 15–240 min after administration, and survival, weight gain, and water and food consumption were analyzed daily. Organ weights and hematological parameters were analyzed after 14 days. Hemolytic activity of MoFTI was tested using Swiss female mice erythrocytes. Splenocytes obtained from BALB/c mice were cultured in the absence or presence of MoFTI for the evaluation of cell viability and proliferation. Mitochondrial membrane potential (ΔΨm) and reactive oxygen species (ROS) levels were also determined. Furthermore, the culture supernatants were analyzed for the presence of cytokines and nitric oxide (NO). Results: MoFTI did not cause death or any adverse effects on the mice except for abdominal contortions at 15–30 min after administration. MoFTI did not exhibit a significant hemolytic effect. In addition, MoFTI did not induce apoptosis or necrosis in splenocytes and had no effect on cell proliferation. Increases in cytosolic and mitochondrial ROS release, as well as ΔΨm reduction, were observed in MoFTI-treated cells. MoFTI was observed to induce TNF-α, IFN-γ, IL-6, IL-10, and NO release. Conclusion: These results contribute to the ongoing evaluation of the antitumor potential of MoFTI and its effects on other immunological targets.

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