scholarly journals Physiological and proteomics responses of nitrogen assimilation and glutamine/glutamine family of amino acids metabolism in mulberry (Morus alba L.) leaves to NaCl and NaHCO3 stress

2020 ◽  
Vol 15 (10) ◽  
pp. 1798108
Author(s):  
Huihui Zhang ◽  
Yuze Huo ◽  
Zisong Xu ◽  
Kaiwen Guo ◽  
Yue Wang ◽  
...  
2002 ◽  
Vol 50 (2) ◽  
pp. 185-192 ◽  
Author(s):  
Genjirou Kusano ◽  
Shiho Orihara ◽  
Daisuke Tsukamoto ◽  
Makio Shibano ◽  
Maksut Coskun ◽  
...  
Keyword(s):  

1949 ◽  
Vol 32 (3) ◽  
pp. 271-277 ◽  
Author(s):  
Richard Abrams ◽  
E. Hammarsten ◽  
P. Reichard ◽  
E. Sperber

By using N15 as a tracer the assimilation of ammonia by the yeast, Torulopsis utilis, has been studied. It has been shown that: 1. There was no measurable incorporation of N in the protein or polynucleotide purine of carbon-starved yeast. 2. When ammonia is added to nitrogen-starved yeast there is a long lag period before division begins during which the yeast rapidly synthesizes protein, this process being accompanied by a turnover of polynucleotide purine. There was no significant dilution of the N15H4+ of the medium by ordinary NH4+. 3. When yeast containing N15 is allowed to divide and grow in ordinary ammonia, the total amount of N15 in the yeast remains constant. The dicarboxylic amino acids are most diluted, while arginine and nucleic acid guanine are not diluted at all.


1994 ◽  
Vol 72 (1) ◽  
pp. 12-14 ◽  
Author(s):  
Yoshio Hano ◽  
Taro Nomura ◽  
Shinichi Ueda

L-[3-13C]Phenylalanine and L-[3-13C]tyrosine were administered to Morus alba cell cultures, to produce intermolecular Diels–Alder type adducts of a prenylchalcone and a 2-arylbenzofuran such as chalcomoracin (1) as well as the adducts of two molecules of prenylchalcones such as kuwanon J (2) in high yields. The 13C NMR spectra of 1 and 2 isolated from the cultures revealed that both amino acids were incorporated intact into chalcomoracin (1) and kuwanon J (2). This is the first example of direct NMR evidence for the almost equivalent incorporation of phenylalanine and tyrosine into the shikimate metabolites. This finding suggests the participation of a biosynthetic route from phenylalanine via trans-cinnamate to p-coumarate and from tyrosine to p-coumarate in this plant.


Author(s):  
H.-M. Lam ◽  
K. T. Coschigano ◽  
I. C. Oliveira ◽  
R. Melo-Oliveira ◽  
G. M. Coruzzi

2012 ◽  
Vol 92 (3) ◽  
pp. 399-405 ◽  
Author(s):  
Bataung Mokhele ◽  
Xianjin Zhan ◽  
Guozheng Yang ◽  
Xianlong Zhang

Mokhele, B., Zhan, X., Yang, G. and Zhang, X. 2012. Review: Nitrogen assimilation in crop plants and its affecting factors. Can. J. Plant Sci. 92: 399–405. In this review we discuss mainly nitrogen assimilation in crop plants and factors affecting the related process. Nitrogen is a major macro-element limiting the growth and development of plants in agriculture. Both organic and inorganic forms of nitrogen are metabolized in plants; nitrate and ammonia in soil are common forms of inorganic nitrogen that can be metabolized in all plants. There are other nitrogen forms, which include amino acids, nitrite and urea, that are metabolized in plants. Metabolism normally starts with reduction of nitrate to nitrite, and the latter further reduces to form ammonium with the presence of relevant enzymes. This reaction occurs more rapidly in leaves in the presence of light. After ammonia is formed, it enters into the biosynthetic pathways of plant cells, such as reductive amination and transpiration, to produce different amino acids. Amino acids in cells take part in the synthesis of protein and other nitrogenous compounds that help in body building. Radiation, gaseous factors, the presence of metals, soil pH and amount of nitrate are some of the environmental factors affecting absorption and reduction of nitrogen in plants. This review presents a comprehensive understanding of the assimilation process by crop plants of nitrogen and recommends that favorable surrounding conditions are the prerequisites for plants to absorb and utilize nitrogen efficiently.


2015 ◽  
Vol 67 (4) ◽  
pp. 1107-1118
Author(s):  
Chenguang Zhu ◽  
Guimin Zhang ◽  
Shilin Chen ◽  
Wei Wang ◽  
Yuanping Tang ◽  
...  

The overexpression of the algal glutamine synthetase (GS) gene DvGS1 in Arabidopsis thaliana resulted in higher plant biomass and better growth phenotype. The purpose of this study was to recognize the biological mechanism for the growth improvement of DvGS1-transgenic Arabidopsis. A series of molecular and biochemical investigations related to nitrogen and carbon metabolism in the DvGS1-transgenic line was conducted. Analysis of nitrogen use efficiency (NUE)-related gene transcription and enzymatic activity revealed that the transcriptional level and enzymatic activity of the genes encoding GS, glutamate synthase, glutamate dehydrogenase, alanine aminotransferase and aspartate aminotransferase, were significantly upregulated, especially from leaf tissues of the DvGS1-transgenic line under two nitrate conditions. The DvGS1-transgenic line showed increased total nitrogen content and decreased carbon: nitrogen ratio compared to wild-type plants. Significant reduced concentrations of free nitrate, ammonium, sucrose, glucose and starch, together with higher concentrations of total amino acids, individual amino acids (glutamate, aspartate, asparagine, methionine), soluble proteins and fructose in leaf tissues confirmed that the DvGS1-transgenic line demonstrated a higher efficiency of nitrogen assimilation, which subsequently affected carbon metabolism. These improved metabolisms of nitrogen and carbon conferred the DvGS1-transgenic Arabidopsis higher NUE, more biomass and better growth phenotype compared with the wild-type plants.


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