Assessment of antioxidant, immune enhancement, and antimutagenic efficacy of fennel seed extracts in irradiated human blood cultures

An 88-year-old man was admitted to the hospital with worsening malaise, fever, and weakness. Anaerobic blood culture bottles revealed the presence of an anaerobic, Gram-positive sporulated bacillus. Empirical antibiotherapy with intravenous piperacillin-tazobactam was initiated. The patient defervesced after four days and was switched to oral amoxicillin on his 6th day of antibiotic therapy and later discharged from the hospital. Four months later, he had recovered. The bacterium was initially identified asClostridium butyricumusing anaerobic manual identification panel. 16S rRNA gene sequence and phylogenetic analysis showed the bacterium to beClostridium lavalense, a recently described species with no previously published case of isolation in human diagnostic samples so far. This is the first report ofClostridium lavalenseisolation from human blood cultures. Further studies are needed in order to elucidate the role ofClostridium lavalensein human disease and its virulence factors.

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Identification of lactic acid bacteria isolated from human blood cultures

FEMS Immunology & Medical Microbiology ◽

10.1111/j.1574-695x.2006.00199.x ◽

2007 ◽

Vol 49 (2) ◽

pp. 192-196 ◽

Cited By ~ 24

Author(s):

Pavel Å vec ◽

Alena Å evÄÃkovÃ¡ ◽

Ivo SedlÃ¡Äek ◽

Jana BednÃ¡Å™ovÃ¡ ◽

Cindy Snauwaert ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Human Blood ◽

Blood Cultures ◽

Human Blood Cultures

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Interleukin 10 secretion in relation to human IL-10 locus haplotypes

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.95.16.9465 ◽

1998 ◽

Vol 95 (16) ◽

pp. 9465-9470 ◽

Cited By ~ 328

Author(s):

Joyce Eskdale ◽

Grant Gallagher ◽

Cor L. Verweij ◽

Vivian Keijsers ◽

Rudi G. J. Westendorp ◽

...

Keyword(s):

Human Blood ◽

Transcription Initiation ◽

Interleukin 10 ◽

Initiation Site ◽

Blood Cultures ◽

Genetic Composition ◽

Promoter Haplotypes ◽

Made In ◽

Stimulation Of ◽

Human Blood Cultures

Stimulation of human blood cultures with bacterial lipopolysaccharide (LPS) shows large inter-individual variation in interleukin 10 (IL-10) secretion, which has been shown to have a genetic component of over 70%. Alleles at two microsatellite loci in the 4 kb immediately upstream of the human IL-10 transcription initiation site in 132 individuals from 56 Dutch families were defined and assigned as haplotypes. LPS-induced IL-10 secretion was measured by ELISA and related to the IL-10 promoter haplotypes present in 78 unrelated individuals obtained from these families. Analysis showed that LPS-induced IL-10 secretion from unrelated individuals varied with IL-10 promoter haplotypes (P= 0.024; Kruskal-Wallis test). Two observations were made in relation to secreted IL-10 levels and promoter haplotypes; first, those haplotypes containing the allele IL10.R3 were associated with lower IL-10 secretion than haplotypes containing any other IL10.R allele. Second, the haplotype IL10.R2/IL10.G14 was associated with highest IL-10 secretion overall, whereas the haplotype IL10.R3/IL10.G7 was associated with lowest IL-10 secretion. These data demonstrate that the ability to secrete IL-10 can vary in man according to the genetic composition of the IL-10 locus.

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