In-vitro stress stability, digestibility and bioaccessibility of curcumin-loaded polymeric nanocapsules

Aim: To elucidate the role of Rv2223c in Mycobacterium tuberculosis. Methods: Purified recombinant Rv2223c protein was characterized. Expression of rv2223c in the presence of different stress environment and subcellular localization were performed in M. tuberculosis H37Ra and Mycobacterium smegmatis ( MS_2223c). Effect of its overexpression on growth rate, infection and intracellular survival in THP-1/PBMC cells were studied. Results: rRv2223c demonstrated esterase activity with preference for pNP-octanoate and hydrolyzed trioctanoate to di- and mono-octanoate. Expression of rv2223c was upregulated in acidic and nutritive stress conditions. rRv2223c was identified in extracellular and cell wall fractions. MS_2223c exhibited enhanced growth, survival during in vitro stress, infection and intracellular survival. Conclusions: Rv2223c is a secretary, carboxyl-esterase, with enhanced expression under acidic and nutritive stress condition and might help in intracellular survival of bacteria.

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N-acetylcysteine protects induced pluripotent stem cells from in vitro stress: impact on differentiation outcome

The International Journal of Developmental Biology ◽

10.1387/ijdb.120070ji ◽

2012 ◽

Vol 56 (9) ◽

pp. 729-735 ◽

Cited By ~ 18

Author(s):

Ina Berniakovich ◽

Leopoldo Laricchia-Robbio ◽

Juan Carlos Izpisua Belmonte

Keyword(s):

Stem Cells ◽

Induced Pluripotent Stem Cells ◽

Pluripotent Stem Cells ◽

Induced Pluripotent ◽

In Vitro Stress

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In vitro stress shielding measurements can be affected by large errors

The Journal of Arthroplasty ◽

10.1016/s0883-5403(99)90129-8 ◽

1999 ◽

Vol 14 (2) ◽

pp. 215-219 ◽

Cited By ~ 24

Author(s):

Luca Cristofolini ◽

Marco Viceconti

Keyword(s):

Stress Shielding ◽

In Vitro Stress

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RNA atlas of human bacterial pathogens uncovers stress dynamics linked to infection

Nature Communications ◽

10.1038/s41467-021-23588-w ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Kemal Avican ◽

Jehad Aldahdooh ◽

Matteo Togninalli ◽

A. K. M. Firoj Mahmud ◽

Jing Tang ◽

...

Keyword(s):

Stress Responses ◽

Large Scale ◽

Bacterial Pathogens ◽

Stress Conditions ◽

Altered Expression ◽

Online Resource ◽

Species Specific ◽

In Vitro Stress

AbstractBacterial processes necessary for adaption to stressful host environments are potential targets for new antimicrobials. Here, we report large-scale transcriptomic analyses of 32 human bacterial pathogens grown under 11 stress conditions mimicking human host environments. The potential relevance of the in vitro stress conditions and responses is supported by comparisons with available in vivo transcriptomes of clinically important pathogens. Calculation of a probability score enables comparative cross-microbial analyses of the stress responses, revealing common and unique regulatory responses to different stresses, as well as overlapping processes participating in different stress responses. We identify conserved and species-specific ‘universal stress responders’, that is, genes showing altered expression in multiple stress conditions. Non-coding RNAs are involved in a substantial proportion of the responses. The data are collected in a freely available, interactive online resource (PATHOgenex).

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