scholarly journals Whole genome analysis unveils genetic diversity and potential virulence determinants in Vibrio parahaemolyticus associated with disease outbreak among cultured Litopenaeus vannamei (Pacific white shrimp) in India

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 1936-1949
Author(s):  
Kattapuni Suresh Prithvisagar ◽  
Ballamoole Krishna Kumar ◽  
Toshio Kodama ◽  
Praveen Rai ◽  
Tetsuya Iida ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genome Analysis ◽  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Disease Outbreak ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Whole Genome ◽  
Virulence Determinants ◽  
Whole Genome Analysis

Diversity of Vibrio parahaemolyticus associated with disease outbreak among cultured Litopenaeus vannamei (Pacific white shrimp) in India

Aquaculture ◽  
2014 ◽  
Vol 433 ◽  
pp. 247-251 ◽  
Author(s):  
Ballamoole Krishna Kumar ◽  
Vijaya Kumar Deekshit ◽  
Juliet Roshini Mohan Raj ◽  
Praveen Rai ◽  
Balligavi Mallappa Shivanagowda ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Disease Outbreak ◽  
Pacific White Shrimp ◽  
White Shrimp

scholarly journals Genetic diversity within and between broodstocks of the white shrimp Litopenaeus vannamei (Boone, 1931) (Decapoda, Penaeidae) and its implication for the gene pool conservation

2007 ◽  
Vol 67 (4 suppl) ◽  
pp. 939-943 ◽  
Author(s):  
PD. Freitas ◽  
MR. Calgaro ◽  
PM. Galetti Jr.
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Litopenaeus Vannamei ◽  
Gene Pool ◽  
Rapd Analysis ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Brazilian Coast ◽  
The Pacific ◽  
Improvement Programs

Genetic variation within and between fifteen closed broodstock lines of the Pacific white shrimp Litopenaeus vannamei, reared at different hatcheries in the Brazilian coast, was assessed by RAPD analysis. Fifty two polymorphic loci were identified when a set of five decamer primers was used in PCR. The genetic diversity analysis within lines evidenced genetic variation loss probably related to bottleneck effects and inbreeding. In addition, the genetic divergence values between the different samples appear to reflect the initial founder composition of such stocks, in some cases, sharing a common origin, suggesting a putative importance of interbreeding for the establishment of genetic improvement programs for these broodstocks. The genetic variation monitoring appears to be helpful to the gene pool conservation of this aquaculture species, mainly if considered its exotic status in Brazil and the current impossibility of new introduction of wild individuals.

scholarly journals Whole-Genome Analysis of Porcine Circovirus Type 2 in Russia

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1631
Author(s):  
Sergei Raev ◽  
Anton Yuzhakov ◽  
Taras Aliper
Keyword(s):  
Genetic Diversity ◽  
Genome Analysis ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Whole Genome ◽  
Whole Genome Analysis ◽  
Reading Frame

Porcine circovirus type 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVAD) that bring about significant economic losses in the pig industry all over the world. The aim of this study was to investigate the genetic diversity of PCV2 in Russia and characterize the available complete genome sequences. PCV2 DNA was detected at all investigated farms located in different regions of Russia. Whole-genome analysis demonstrated that the majority of PCV2 strains belonged to genotype PCV2d (12 out of 14), while PCV2a and PCV2b were only detected at 2 farms (one at each). Further analysis revealed that all antibody recognition sites in Russian PCV2 strains were different from the corresponding epitopes in a PCV2a vaccine strain, suggesting that PCV2a-based vaccines may only provide limited protection against these strains. PCV2d strains could be grouped into 3 distinct lines which shared 98.7–100% identity within open reading frame 2 (ORF2). It is the first study reporting the genetic diversity of PCV2 strains in Russia. Our data indicated that, similarly to China, Europe, and USA, PCV2a and PCV2b have largely been replaced by PCV2d.

Whole genome analysis of porcine astroviruses detected in Japanese pigs reveals genetic diversity and possible intra-genotypic recombination

2017 ◽  
Vol 50 ◽  
pp. 38-48 ◽  
Author(s):  
Mika Ito ◽  
Moegi Kuroda ◽  
Tsuneyuki Masuda ◽  
Masataka Akagami ◽  
Kei Haga ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genome Analysis ◽  
Whole Genome ◽  
Whole Genome Analysis

scholarly journals Effects of spray-dried animal plasma on growth performance, survival, feed utilization, immune responses, and resistance to Vibrio parahaemolyticus infection of Pacific white shrimp (Litopenaeus vannamei)

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257792
Author(s):  
Niti Chuchird ◽  
Tirawat Rairat ◽  
Arunothai Keetanon ◽  
Putsucha Phansawat ◽  
Chi-Chung Chou ◽  
...  
Keyword(s):  
Immune Responses ◽  
Growth Performance ◽  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Positive Control ◽  
Feed Utilization ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Total Hemocyte Count ◽  
Spray Dried

Spray-dried animal plasma (SDP) in feed for several animal species provides health benefits, but research about use of SDP in shrimp feed is very limited. The objectives of the present study were to investigate the effects of dietary SDP on growth performance, feed utilization, immune responses, and prevention of Vibrio parahaemolyticus infection in Pacific white shrimp (Litopenaeus vannamei). In Experiment 1, the post-larvae were divided into five groups (four tank/group and 80 shrimp/tank) and fed four times daily diets with porcine SDP at 0, 1.5, 3, 4.5, and 6% of the diet for 45 days. In Experiment 2, the surviving shrimp from Experiment 1 were redistributed into six groups: four SDP groups as in Experiment 1 plus the positive and negative controls (four tank/group and 30 shrimp/tank). They were then challenged with V. parahaemolyticus by immersion at 105 colony-forming units (CFU)/mL and were fed with the same diets for another 4 days. In Experiment 1, shrimp fed 4.5% or 6% SDP diets had significantly higher body weight, survival rate, and improved feed conversion ratio. The immune parameters (total hemocyte count and phagocytic, phenoloxidase, and superoxide dismutase activities) of the shrimp fed 3–6% SDP diets also showed significant enhancement compared to the control. In Experiment 2, the survival rates of the 3–6% SDP groups were significantly higher than the positive control at day 4 after the immersion challenge. Likewise, the histopathological study revealed milder signs of bacterial infection in the hepatopancreas of the 3–6% SDP groups compared to the challenged positive control and 1.5% SDP groups. In conclusion, shrimp fed diets with SDP, especially at 4.5–6% of the diet, showed significant improvement in overall health conditions and better resistance to V. parahaemolyticus infection.

scholarly journals Evaluation of the Dietary Black Soldier Fly Larvae Meal (Hermetia illucens) on Growth Performance, Intestinal Health, and Disease Resistance to Vibrio parahaemolyticus of the Pacific White Shrimp (Litopenaeus vannamei)

2021 ◽  
Vol 8 ◽  
Author(s):  
Yongkang Chen ◽  
Shuyan Chi ◽  
Shuang Zhang ◽  
Xiaohui Dong ◽  
Qihui Yang ◽  
...  
Keyword(s):  
Growth Performance ◽  
Intestinal Microbiota ◽  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Mrna Levels ◽  
Black Soldier Fly ◽  
The Pacific ◽  
Black Soldier Fly Larvae

The present study evaluated the effects of the dietary black soldier fly larvae meal (BSF) on growth performance, intestinal health, and susceptibility to Vibrio parahaemolyticus in the Pacific white shrimp Litopenaeus vannamei. The basal diet was formulated to contain 25% fish meal (FM), and then the FM was replaced with BSF for 10, 20, and 30% of the FM protein in the experimental diets, which are referred to as FM, BSF10, BSF20, and BSF30, respectively. Four hundred and eighty healthy and uniform-sized shrimp (~0.88 g) were distributed among four groups of three replicates, each with 40 shrimp in a 300-L tank and they were fed four times daily for 7 weeks. The results showed that the growth performance did not change significantly in shrimp fed with BSF10 and BSF20 diets, but significantly decreased in those fed with BSF30 diet compared to the ones fed with FM diet. After feeding trial and sampling, a V. parahaemolyticus infection challenge trial was conducted on shrimp. The results showed that the survival rate of shrimp fed with BSF10 was significantly higher than those fed with FM. The results of the midgut histology showed that the width and height of intestinal mucosal folds decreased significantly in shrimp fed with BSF20 and BSF30, and the early signs of apoptosis in the intestinal cells were found in shrimp fed with BSF30. The mRNA levels of non-specific immune-related genes dorsal and relish were downregulated in shrimp fed with BSF20 and BSF30 diets. The mRNA levels of antimicrobial peptides–related genes alf (anti-lipopolysaccharide factor) were upregulated in shrimp fed with BSF10 but downregulated in shrimp fed with BSF30. The mRNA level pen3 (penaeidins 3) was upregulated in shrimp fed with BSF10 and BSF20 diets. The intestinal bacterial communities on operational taxonomic unit levels among groups were not significantly differentiated according to the beta diversity analysis. At the genus levels, a decrease in Vibrio, Photobacterium, and Candidatus_Bacilloplasma, as well as the increase in Bacillus and Pseudoalteromonas abundance, indicated the improvement of intestinal microbiota in shrimp fed with dietary BSF. Therefore, the use of BSF in shrimp diet should be controlled at a dosage of 20% of the FM, which can improve the intestinal microbiota without causing any negative effects.

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