scholarly journals The protective effects of dezocine on interleukin-1β-induced inflammation, oxidative stress and apoptosis of human nucleus pulposus cells and the possible mechanisms

Bioengineered ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 1399-1410
Author(s):  
Fang Zhu ◽  
Wei Duan ◽  
Chao Zhong ◽  
Bing Ji ◽  
Xinjun Liu
Keyword(s):  
Oxidative Stress ◽  
Nucleus Pulposus ◽  
Interleukin 1Β ◽  
Protective Effects ◽  
Nucleus Pulposus Cells

Melatonin resists oxidative stress-induced apoptosis in nucleus pulposus cells

Life Sciences ◽  
2018 ◽  
Vol 199 ◽  
pp. 122-130 ◽  
Author(s):  
Ruijun He ◽  
Min Cui ◽  
Hui Lin ◽  
Lei Zhao ◽  
Jiayu Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nucleus Pulposus ◽  
Nucleus Pulposus Cells ◽  
Induced Apoptosis

scholarly journals Liraglutide inhibits the apoptosis of human nucleus pulposus cells induced by high glucose through PI3K/Akt/caspase-3 signaling pathway

2019 ◽  
Vol 39 (8) ◽  
Author(s):  
Yao Ming-yan ◽  
Zhang Jing ◽  
Guo Shu-qin ◽  
Bai Xiao-liang ◽  
Li Zhi-hong ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Nucleus Pulposus ◽  
High Glucose ◽  
Caspase 3 ◽  
Maximum Effect ◽  
Ros Generation ◽  
Protective Effects ◽  
Small Interfering Rnas ◽  
Nucleus Pulposus Cells ◽  
Pi3k Inhibitor Ly294002

Abstract Diabetes mellitus (DM) is a potential etiology of disc degeneration. Glucagon-like peptide-1 (GLP-1) is currently regarded as a powerful treatment option for type 2 diabetes. Apart from the beneficial effects on glycaemic control, GLP-1 has been reported to exert functions in a variety of tissues on modulation of cell proliferation, differentiation, and apoptosis. However, little is known regarding the effects of GLP-1 on nucleus pulposus cells (NPCs). In the present study, we investigated the effects of liraglutide (LIR), a long-lasting GLP-1 analogue, on apoptosis of human NPCs and the underlying mechanisms involved. We confirmed the presence of GLP-1 receptor (GLP-1R) in NPCs. Our data demonstrated that liraglutide inhibited the apoptosis of NPCs induced by high glucose (HG), as detected by Annexin V/Propidium Iodide (PI) and ELISA assays. Moreover, liraglutide down-regulated caspase-3 activity at intermediate concentration (100 nM) for maximum effect. Further analysis suggested that liraglutide suppressed reactive oxygen species (ROS) generation and stimulated the phosphorylation of Akt under HG condition. Pretreatment of cells with the Phosphoinositide 3-kinase (PI3K) inhibitor LY294002 (LY) and small interfering RNAs (siRNAs) GLP-1R abrogated the liraglutide-induced activation of Akt and the protective effects on NPCs’ apoptosis. In conclusion, liraglutide could directly protect NPCs against HG-induced apoptosis by inhibiting oxidative stress and activate the PI3K/Akt/caspase-3 signaling pathway via GLP-1R.

scholarly journals Caveolin-1 regulates oxidative stress-induced senescence in nucleus pulposus cells primarily via the p53/p21 signaling pathway in vitro

2017 ◽  
Vol 16 (6) ◽  
pp. 9521-9527 ◽  
Author(s):  
Lei Ding ◽  
Qingmin Zeng ◽  
Jingping Wu ◽  
Defang Li ◽  
Houlei Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Signaling Pathway ◽  
Nucleus Pulposus ◽  
Nucleus Pulposus Cells ◽  
Caveolin 1

Hyaluronic Acid Based Hydrogels Attenuate Inflammatory Receptors and Neurotrophins in Interleukin-1β Induced Inflammation Model of Nucleus Pulposus Cells

2015 ◽  
Vol 16 (6) ◽  
pp. 1714-1725 ◽  
Author(s):  
Isma Liza Mohd Isa ◽  
Akshay Srivastava ◽  
David Tiernan ◽  
Peter Owens ◽  
Peadar Rooney ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Nucleus Pulposus ◽  
Interleukin 1Β ◽  
Nucleus Pulposus Cells ◽  
Inflammation Model

scholarly journals Icariin Prevents H2O2-Induced Apoptosis via the PI3K/Akt Pathway in Rat Nucleus Pulposus Intervertebral Disc Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Xiangyu Deng ◽  
Sheng Chen ◽  
Dong Zheng ◽  
Zengwu Shao ◽  
Hang Liang ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Caspase 3 ◽  
Chinese Herb ◽  
Akt Pathway ◽  
Control Group ◽  
Protective Effects ◽  
Nucleus Pulposus Cells ◽  
Intracellular Ros ◽  
Induced Apoptosis

Icariin is a prenylated flavonol glycoside derived from the Chinese herb Epimedium sagittatum. This study investigated the mechanism by which icariin prevents H2O2-induced apoptosis in rat nucleus pulposus (NP) cells. NP cells were isolated from the rat intervertebral disc and they were divided into five groups after 3 passages: (A) blank control; (B) 200 μM H2O2; (C) 200 μM H2O2 + 20 μM icariin; (D) 20 μM icariin + 200 μM H2O2 + 25 μM LY294002; (E) 200 μM H2O2 + 25 μM LY294002. LY294002 is a selective inhibitor of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. NP cell viability, apoptosis rate, intracellular reactive oxygen species levels, and the expression of AKT, p-AKT, p53, Bcl-2, Bax, caspase-3 were estimated. The results show that, compared with the control group, H2O2 significantly increased NP cell apoptosis and the level of intracellular ROS. Icariin pretreatment significantly decreased H2O2-induced apoptosis and intracellular ROS and upregulated p-Akt and BCL-2 and downregulated caspase-3 and Bax. LY294002 abolished the protective effects of icariin. Our results show that icariin can attenuate H2O2-induced apoptosis in rat nucleus pulposus cells and PI3K/AKT pathway is at least partly included in this protection effect.

The effects of ferulic acid on nucleus pulposus cells under hydrogen peroxide-induced oxidative stress

2011 ◽  
Vol 46 (8) ◽  
pp. 1670-1677 ◽  
Author(s):  
Yung-Hsin Cheng ◽  
Shu-Hua Yang ◽  
Kai-Chiang Yang ◽  
Moon-Pei Chen ◽  
Feng-Huei Lin
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Ferulic Acid ◽  
Nucleus Pulposus ◽  
Nucleus Pulposus Cells
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