Molecular characteristics of mitochondrial DNA and phylogenetic analysis of the hybrid loach of Misgurnus anguillicaudatus (female) and Paramisgurnus dabryanus ssp. (male)

Cytochrome b (cyt b) is one of the genes in mitochondrial DNA that is often used as a molecular marker to identify species through DNA Barcoding. The aim of the present study was to investigate the bioinformatic of cyt b that isolated from mackerel fish. PCR amplification showed the length of DNA cyt b from king mackerel was 803 bp within purine 312 bp and pyrimidine 491 bp while Korean mackerel 791 bp within purine 316 bp and pyrimidine 475 bp. Phylogenetic analysis showed all sample join in mackerel groups (Scomberomorus commerson and Scomberomorus koreanus). The Isoelectric point value of cyt b from king mackerel is 6.38 and molecular weight is 29826.23; Korean mackerel are 8.67 and molecular weight is 29372.77. Hydrophaty plot showed cyt b of mackerel more hydrophobic. Based on 3D modelling both of them have eight different sections showing by different colors. Keywords: characteristic molecular, cytochrome b, Mackerel

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Molecular characteristics of mitochondrial DNA and phylogenetic analysis of the hybrid loach of Oxygymnocypris stewartii (female) and Schizopygopsis younghusbandi (male)

Mitochondrial DNA Part B ◽

10.1080/23802359.2020.1742614 ◽

2020 ◽

Vol 5 (2) ◽

pp. 1498-1499

Author(s):

Wanliang Wang ◽

Chi Zhang ◽

Bo Ma

Keyword(s):

Phylogenetic Analysis ◽

Mitochondrial Dna ◽

Molecular Characteristics ◽

Oxygymnocypris Stewartii

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First Insight into Groundwater Copepods of the Polish Lowland

Water ◽

10.3390/w13152086 ◽

2021 ◽

Vol 13 (15) ◽

pp. 2086

Author(s):

Maciej Karpowicz ◽

Sabina Smolska ◽

Magdalena Świsłocka ◽

Joanna Moroz

Keyword(s):

Mitochondrial Dna ◽

Ribosomal Rna ◽

Dna Markers ◽

Coi Gene ◽

Molecular Characteristics ◽

Morphological Description ◽

Single Plate ◽

North Eastern ◽

Insight Into

Our results are the first insight into groundwater copepods of the Polish Lowland. The sampling was conducted in 28 wells in north-eastern Poland, and Copepoda were present in 16 wells. We have identified six Copepoda species and one Cladocera. We have classified four species as stygophiles—Eucyclops serrulatus, Diacyclops bisetosus, Diacyclops crassicaudis, and Cyclops furcifer. These species were frequently found in studied wells of different regions of north-eastern Poland, often in high numbers, and females with egg sacs were observed. We present a detailed morphological description of these species, together with molecular characteristics based on mitochondrial DNA markers (COI gene) for E. serrulatus, D. bisetosus, and D. crassicaudis, and 12S ribosomal RNA for C. furcifer. We also present the development of abnormal structures in one specimen of D. crassicaudis, where the upper part of furcal rami was fused to form a single plate.

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Morphological and Phylogenetic Analysis of Eustrongylides sp. and Gnathostoma spinigerum Parasitizing the Asian Swamp Eel Monopterusalbus in China

Pathogens ◽

10.3390/pathogens10060711 ◽

2021 ◽

Vol 10 (6) ◽

pp. 711

Author(s):

Sixin Zhang ◽

Guangping Huang ◽

Liang Li ◽

Xianyong Liu ◽

Xiaoli Tang ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Coi Gene ◽

Molecular Characteristics ◽

Mean Intensity ◽

Gnathostoma Spinigerum ◽

Zoonotic Parasite ◽

Third Stage ◽

Parasite Infections ◽

Asian Swamp Eel ◽

Swamp Eel

Nematode infections transmitted to humans by the consumption of wild or cultured eels are increasingly being reported. In the present study, 120 Asian swamp eel, Monopterus albus (Zuiew), individuals collected from China were examined for parasite infections, and 78 larval nematodes were isolated. Morphological and molecular characteristics, including sequence and phylogenetic analysis of the internal transcribed spacer (ITS) and cytochrome c oxidase subunit I (COI) gene regions, were employed to identify these nematodes at the lowest taxonomic level possible. Asian swamp eel was infected with two zoonotic parasite taxa: Gnathostoma spinigerum advanced third-stage larvae, with 6.67% prevalence and mean intensity = 1.25, and Eustrongylides sp. fourth-stage larvae, with 26.67% prevalence and mean intensity = 2.13. These findings evidence the need to enhance public hygiene and food safety awareness toward eel consumption

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Species delimitation of colour polymorphic Cladophorus (Coleoptera: Lycidae) from New Guinea

Zootaxa ◽

10.11646/zootaxa.4320.3.6 ◽

2017 ◽

Vol 4320 (3) ◽

pp. 505 ◽

Cited By ~ 4

Author(s):

ROMANA KALOUSOVA ◽

LADISLAV BOCAK

Keyword(s):

Phylogenetic Analysis ◽

Mitochondrial Dna ◽

Related Species ◽

Species Delimitation ◽

Morphological Differentiation ◽

Morphological Characters ◽

Molecular Data ◽

Closely Related Species ◽

Dna Fragment ◽

Colour Patterns

Cladophorus Guérin-Méneville, 1830 are endemic Papuan net-winged beetles which take part in highly diverse Müllerian mimicry rings. Available specimens were sequenced for cox1–tRNA-Leu–cox2 mitochondrial DNA fragment and the species delimitations were based on the genetic distance, phylogenetic analysis, and morphology. Three earlier described species were identified in the recently collected material and further 10 species are described: C. pallescens sp. nov., C. bicolor sp. nov., C. craterensis sp. nov., C. motykai sp. nov., C. mindikensis sp. nov., C. kailakiensis sp. nov., C. manokwarensis sp. nov., C. haiaensis sp. nov., C. humeralis sp. nov., and C. boceki sp. nov. DNA-based identifications provided some ambiguous results and closely related species could not be robustly delimited using solely molecular data. Additionally, the species limits were based on clearly defined morphological characters and the morphological differentiation was found unlinked from the genetic divergence. Colour patterns cannot be used for identification because all species available in more specimens were polymorphic and followed various local co-mimics. The Papuan fauna of Cladophorus is very diverse and the closely related species regularly occur in limited regions. Differentiation within restricted ranges is therefore considered as the main speciation mode.

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Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey

Advances in Virology ◽

10.1155/2019/7163747 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

Gözde Erkiş-Güngör ◽

Bayram Çevik

Keyword(s):

Phylogenetic Analysis ◽

Citrus Tristeza Virus ◽

Molecular Characteristics ◽

Cloning And Sequencing ◽

Phylogenetic Groups ◽

The 1960S ◽

Tristeza Virus ◽

Virus Isolates ◽

Stem Pitting ◽

Citrus Tristeza

The presence of Citrus tristeza virus (CTV) in Turkey has been known since the 1960s and the virus was detected in all citrus growing regions of the country. Even though serological and biological characteristics of CTV have been studied since the 1980s, molecular characteristics of CTV isolates have not been studied to date in Turkey. In this study, molecular characteristics of 15 CTV isolates collected from different citrus growing regions of Turkey were determined by amplification, cloning, and sequencing of their major coat protein (CP) genes. The sequence analysis showed that the CP genes were highly conserved among Turkish isolates. However, isolates from different regions showed more genetic variation than isolates from the same region. Turkish isolates were clustered into three phylogenetic groups showing no association with geographical origins, host, or symptoms induced in indicator plants. Phylogenetic analysis of Turkish isolates with isolates from different citrus growing regions of the world including well-characterized type isolates of previously established strain specific groups revealed that some Turkish isolates were closely related to severe quick decline or stem pitting isolates. The results demonstrated that although CTV isolates from Turkey are considered biologically mild, majority of them contain severe components potentially causing quick decline or stem pitting.

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Species delimitation in the leaf beetle genus Macroplea (Coleoptera, Chrysomelidae) based on mitochondrial DNA, and phylogeographic considerations

Insect Systematics & Evolution ◽

10.1163/187631206788831443 ◽

2006 ◽

Vol 37 (4) ◽

pp. 467-479 ◽

Cited By ~ 10

Author(s):

Gregor Kölsch ◽

Bo Vest Pedersen ◽

Olof Biström

Keyword(s):

Phylogenetic Analysis ◽

Mitochondrial Dna ◽

Species Delimitation ◽

Mitochondrial Gene ◽

Leaf Beetle ◽

Genetic Distances ◽

Amino Acid Substitutions ◽

Species Status ◽

Leaf Beetles ◽

Gene Coding

AbstractThe genus Macroplea Samouelle, 1819 is a group of highly specialized aquatic leaf beetles occurring in the Palaearctic. Since the members of this genus are morphologically very similar, we addressed the question of species identification and delimitation by analysing the second half of the mitochondrial gene coding for the cytochrome oxidase I (COI) subunit. Species limits are inferred from the multimodal frequency distribution of genetic distances between specimens: low genetic distances within a species are clearly set apart from distances between species. The species status of the hitherto controversial species M. japana (Jacoby, 1885) is confirmed. The pattern of nucleotide and amino acid substitutions is discussed in the light of functional domains of the COI molecule. Although the data are preliminary, the results provide new data on the distribution of the species. Together with the phylogenetic analysis they allow for a discussion of the phylogeography of the genus.

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