Species delimitation of colour polymorphic Cladophorus (Coleoptera: Lycidae) from New Guinea

Zootaxa ◽  
2017 ◽  
Vol 4320 (3) ◽  
pp. 505 ◽  
Author(s):  
ROMANA KALOUSOVA ◽  
LADISLAV BOCAK

Cladophorus Guérin-Méneville, 1830 are endemic Papuan net-winged beetles which take part in highly diverse Müllerian mimicry rings. Available specimens were sequenced for cox1–tRNA-Leu–cox2 mitochondrial DNA fragment and the species delimitations were based on the genetic distance, phylogenetic analysis, and morphology. Three earlier described species were identified in the recently collected material and further 10 species are described: C. pallescens sp. nov., C. bicolor sp. nov., C. craterensis sp. nov., C. motykai sp. nov., C. mindikensis sp. nov., C. kailakiensis sp. nov., C. manokwarensis sp. nov., C. haiaensis sp. nov., C. humeralis sp. nov., and C. boceki sp. nov. DNA-based identifications provided some ambiguous results and closely related species could not be robustly delimited using solely molecular data. Additionally, the species limits were based on clearly defined morphological characters and the morphological differentiation was found unlinked from the genetic divergence. Colour patterns cannot be used for identification because all species available in more specimens were polymorphic and followed various local co-mimics. The Papuan fauna of Cladophorus is very diverse and the closely related species regularly occur in limited regions. Differentiation within restricted ranges is therefore considered as the main speciation mode. 

2018 ◽  
Vol 46 (2) ◽  
pp. 239-253 ◽  
Author(s):  
Shilpi Saha ◽  
Sanjay Chakraborty ◽  
Subrina Sehrin ◽  
Abdullah Al Masud ◽  
Kazi Ahsan Habib ◽  
...  

Investigations on Snapper fish of the genus Lutjanus were conducted from October 2015 to April 2016 in the coral ecosystem of St. Martin’s Island, Bangladesh. Twenty one individuals of 8 species of Lutjanus were collected from local fishermen. Both morphological characters and DNA barcoding approach by mitochondrial cytochrome oxidase I subunit (COI) were used to confirm the identification of all species. Present study recorded the first national occurrence of two reef associated Lutjanus species (L. xanthopinnis and L. indicus). In addition six species (L. rivulatus, L. lutjanus, L. vitta, L. lemniscatus, L. fulviflamma and L. johnii) were re-described. Distinct morphological differentiation were found between more closely related species L. xanthopinnis, L. vitta and L. lutjanus. Three different appearances of L. johnii, two different appearances of L. rivulatus and L. lemniscatus were confirmed as same species. Phylogenetic analysis showed monophylotic clade for conspecific individuals and paraphyletic clade for congeneric individuals. Average genetic divergence for intraspecific level was 1.5% and 12.5% for interspecific level. Current study increased the number of Lutjanus species in Bangladesh from 15 to 17 and extended the distribution range of those new recorded species in northeastern part of the Bay of Bengal. Bangladesh J. Zool. 46(2): 239-253, 2018


Phytotaxa ◽  
2016 ◽  
Vol 280 (1) ◽  
pp. 1 ◽  
Author(s):  
ALFREDO VIZZINI ◽  
MIRCA ZOTTI ◽  
MIDO TRAVERSO ◽  
ENRICO ERCOLE ◽  
PIERRE-ARTHUR MOREAU ◽  
...  

Several collections of Amanita species from section Vaginatae have been reported to be strictly associated with Helianthemum plants growing in grasslands, a still largely under-explored ectomycorrhizal habitat. The main aim of this study was to investigate the taxonomic status and phylogenetic position of strictly Helianthemum-associated Amanita sect. Vaginatae taxa, informally named Amanita “helianthemicola”. Collections from Italy, England and France were included in this study. The morphologically closely related species complex A. lividopallescens was also examined. Analyses were carried out based on both morphological and molecular data (phylogenetic analysis of the nrITS sequences). All investigated Amanita collections, which are strictly associated with Helianthemum nummularium, turned out to be conspecific with A. simulans. Amanita simulans was recently described from Sardinia (Italy) from Populus nigra habitats based on morphological characters only. As the holotype of A. simulans was lost, a neotype is designated here based on a voucher from the original collecting area. Amanita simulans is re-described, and an extensive discussion on the morphological variability, host species range, distribution and related taxa is provided. Amanita lividopallescens was confirmed as a good species, and re-delimited based on our phylogenetic analysis; moreover, it was epitypified with a recent and well-documented collection from Corse (France). Amanita stenospora is a synonym of A. lividopallescens, being a colour form of the latter without taxonomic value.


Zootaxa ◽  
2019 ◽  
Vol 4691 (4) ◽  
pp. 301-332 ◽  
Author(s):  
ANDREW G. CANNIZZARO ◽  
THOMAS R. SAWICKI

Crangonyx ephemerus n. sp. and Crangonyx pseudoephemerus n. sp. are described from the headwaters of the St. Marks River in Leon County, Florida, based on detailed morphological and molecular comparisons with the closely related species Crangonyx floridanus Bousfield, 1963. The morphological and molecular data, including three species delimitation models, lend support to the hypothesis that the taxon C. floridanus sensu lato represents a species complex. Diagnostic morphological characteristics are highlighted and discussed within this group to assist in future morphological analyses. 


Zootaxa ◽  
2009 ◽  
Vol 2106 (1) ◽  
pp. 51-60 ◽  
Author(s):  
HSI-TE SHIH ◽  
JHY-YUN SHY

A new species of potamid freshwater crab, Geothelphusa makatao, is described from southwestern Taiwan, based on morphological characters and mitochondrial DNA evidence. The new species differs from close congeners, G. pingtung Tan & Liu, 1998, G. shernshan Chen, Cheng & Shy, 2005, and G. ancylophallus Shy, Ng & Yu, 1994 and a superficially similar species, G. albogilva Shy, Ng & Yu, 1994, in the structure of its ambulatory legs, thoracic sternum, male abdomen, and male first pleopods. The unique gene sequences of 16S rRNA and cytochrome oxidase I also support the recognition of this coastal population, which is isolated from other closely related species inhabiting montane areas. Except for G. makatao, the phylogenetic analysis showed that there are three additional hill subclades within the G. pingtung clade, G. shernshan, G. pingtung and the Liangshan subclade, situated in different watersheds of rivers or streams near the Central Range in the southwestern Taiwan.


Phytotaxa ◽  
2015 ◽  
Vol 205 (3) ◽  
pp. 168 ◽  
Author(s):  
Hyun Lee ◽  
Myung Soo Park ◽  
Paul Eunil Jung ◽  
Jonathan J. Fong ◽  
Seung-Yoon Oh ◽  
...  

A new species belonging to Lactarius subg. Plinthogalus was discovered during a long-term project on the diversity of Korean Lactarius. This species is proposed here as Lactarius cucurbitoides. The status of L. cucurbitoides as a new species is supported by molecular data and morphological features. Phylogenetic analysis based on internal transcribed spacer (ITS) sequences shows that L. cucurbitoides is closely related to L. subplinthogalus, L. friabilis, and L. oomsisiensis, with pairwise distances of 2.8–4.3%. Morphological characters of L. cucurbitoides that distinguish it from these closely related species are a pale yellow to pale orange colored pileus and non-discoloration of white latex. The new species is described and illustrated in the present paper.


Zootaxa ◽  
2008 ◽  
Vol 1801 (1) ◽  
pp. 1 ◽  
Author(s):  
LU SUN ◽  
W. P. MCCAFFERTY

The mayfly subfamily Brachycercinae (Pannota: Caenidae) is redefined and shown to be an apotypic, monophyletic group based on numerous synapomorphies, including, for example in the larvae, the presence of ocellar tubercles, an anterior row of long setae on the larval head capsule, a patch of long setae posterior to the base of the glossae, a broad prosternum, and the absence of toothlike claw denticles. Over 100 morphological characters of larvae, adults, and eggs discovered to be useful for phylogenetic analysis and diagnoses are detailed and illustrated. Thirty-eight species are recognized among the genera Brachycercus Curtis, Caenoculis Soldán, Cercobrachys Soldán, Insulibrachys Soldán, Sparbarus, n. gen., Oriobrachys, n. gen., Latineosus, n. gen., Susperatus, n. gen., and Alloretochus, n. gen., by adopting a strictly phylogenetic classification, including a first tribal classification within the subfamily. Brachycercus ojibwe, n. sp., Cercobrachys fox, n. sp., C. lilliei, n. sp., C. pomeiok, n. sp., C. winnebago, n. sp., Latineosus cayo, n. sp., L. cibola, n. sp., Oriobrachys mahakam, n. sp., Sparbarus choctaw, n. sp., S. coushatta, n. sp., S. miccosukee, n. sp., and Susperatus tonkawa, n. sp. are newly described. The Nearctic Brachycercus articus Soldán, n. syn., and B. edmundsi Soldán, n. syn., are shown to be equivalent to B. harrisella Curtis, proving the latter to be a widespread Holarctic species. Sparbarus capnicus (Zhou, Sun and McCafferty), n. comb., S. corniger (Kluge), n. comb., S. europaeus (Kluge), n. comb., S. gilliesi (Soldán and Landa), n. comb., S. japonicus (Gose), n. comb., S. kabyliensis (Soldán), n. comb., S. lacustris (Needham), n. comb., S. maculatus (Berner), n. comb., S. nasutus (Soldán), n. comb., S. tubulatus (Tshernova), n. comb., Susperatus prudens (McDunnough), n. comb., and S. tuberculatus (Soldán), n. comb., are transferred from Brachycercus. Alloretochus peruanicus (Soldán), n. comb., and Latineosus colombianus (Soldán), n. comb., are transferred from Cercobrachys. Caenis dangi (Soldán), n. comb., is transferred from Caenoculis and Brachycercinae to Caeninae, and provisionally placed in Caenis Stephens. Sparbarus flavus (Traver), n. comb., is transferred from Brachycercus and considered to be a nomen dubium. Nomenclatural history, new or revised descriptions as appropriate, diagnoses, illustrations, and keys are provided for known stage of species. Origins and evolutionary relationships of the Brachycercinae are hypothesized based on cladistic results. Brachycercinae is considered to have originated from a Caenis-like ancestor. The genera Cae-WORLD BRACHYCERCINAEnoculis and Insulibrachys represent more ancestral lineages, whereas the genera Sparbarus, n. gen., Brachycercus, Oriobrachys, n. gen., Latineosus, n. gen., Susperatus, n. gen., Alloretochus, n. gen., and Cercobrachys appear more derived. Cercobrachys pomeiok and other closely related species are most apotypic in terms of numbers of accumulated apomorphies.


Zootaxa ◽  
2021 ◽  
Vol 4926 (3) ◽  
pp. 401-416
Author(s):  
MOHAMMAD HUSSAIN FALAHZADAH ◽  
EBRAHIM SHOKOOHI ◽  
GHOLAM HOSSEIN MORAVEJ ◽  
PHATU WILLIAM MASHELA ◽  
ABDUL KHALID MADADI ◽  
...  

Several soil samples from different habitats in Badakhshan province of Afghanistan were collected to isolate and characterize bacteria feeding nematodes. The Galleria mellonella-baiting method was used for the isolation of the Afghan insect-associated nematodes. The nematodes were studied using morphological and morphometric data. The Oscheius specimen was characterized by a longer body (630–820 µm) and shorter pharynx (125–145 µm), whereas other morphological characters were not unusual. The Diploscapter specimen had an annulated cuticle, with lip region width 1.5 times shorter than the stoma, and had separated pharyngeal corpus from the isthmus and vulva located in the middle of the body. The molecular data were derived using three loci; 18S, 28S (D2/D3 segment), and ITS rRNA region, which were utilized to measure the genetic distance. The phylogenetic analysis was conducted to reconstruct the relationship tree. Both morphological and molecular approaches confirmed the identity of nematode isolates as Oscheius tipulae and Diploscapter coronatus. This is the first report of insect-associated nematodes from the soil of Afghanistan. Both species were capable of infecting and killing G. mellonella larvae in less than 96 h. 


Phytotaxa ◽  
2018 ◽  
Vol 350 (1) ◽  
pp. 1
Author(s):  
RUI-HONG WANG ◽  
MAO-QIN XIA ◽  
JIN-BO TAN ◽  
CHUAN CHEN ◽  
XIN-JIE JIN ◽  
...  

A new species, Scrophularia jinii (Scrophulariaceae), from Central China is described and illustrated. This new species was formerly misidentified as S. fargesii, from which it differs in many morphological characters. Moreover, it is distinct with all known Scrophularia species in its unique deeply double serrate leaf margin with 3–7 big teeth on each side. Molecular phylogenetic analysis further supports its species delimitation and suggests a close relationship with several Japanese and North American species.


2006 ◽  
Vol 37 (4) ◽  
pp. 467-479 ◽  
Author(s):  
Gregor Kölsch ◽  
Bo Vest Pedersen ◽  
Olof Biström

AbstractThe genus Macroplea Samouelle, 1819 is a group of highly specialized aquatic leaf beetles occurring in the Palaearctic. Since the members of this genus are morphologically very similar, we addressed the question of species identification and delimitation by analysing the second half of the mitochondrial gene coding for the cytochrome oxidase I (COI) subunit. Species limits are inferred from the multimodal frequency distribution of genetic distances between specimens: low genetic distances within a species are clearly set apart from distances between species. The species status of the hitherto controversial species M. japana (Jacoby, 1885) is confirmed. The pattern of nucleotide and amino acid substitutions is discussed in the light of functional domains of the COI molecule. Although the data are preliminary, the results provide new data on the distribution of the species. Together with the phylogenetic analysis they allow for a discussion of the phylogeography of the genus.


Zootaxa ◽  
2012 ◽  
Vol 3303 (1) ◽  
pp. 59 ◽  
Author(s):  
CARLOS PRIETO ◽  
GABRIEL RODRIGUEZ

A new butterfly species, Podanotum pulsar Prieto & Rodríguez n. sp. is described from the high Andes of Colombia. Wepresent a brief discussion on its systematic placement and on adult ecology. Adult specimens and the genitalia of the male andfemale are illustrated and compared with the most closely related species Podanotum glorissimum Bálint & Wojtusiak, according to morphological characters.


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