scholarly journals Charophytes in warm springs on Svalbard (Spitsbergen): DNA barcoding identifies Chara aspera and Chara canescens with unusual morphological traits

2019 ◽  
Vol 167 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Anders Langangen ◽  
Andreas Ballot ◽  
Petra Nowak ◽  
Susanne C. Schneider
Keyword(s):  
Dna Barcoding ◽  
Morphological Traits ◽  
Chara Aspera ◽  
Warm Springs

Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding

2014 ◽  
Vol 59 (2) ◽  
Author(s):  
Bjørn Rukke ◽  
Symira Cholidis ◽  
Arild Johnsen ◽  
Preben Ottesen
Keyword(s):  
Dna Barcoding ◽  
Morphological Traits ◽  
Data Systems ◽  
Morphological Classification ◽  
Northern Norway ◽  
Life Data ◽  
Summer Holiday ◽  
Practical Tool ◽  
Hypoderma Tarandi

AbstractDNA barcoding is a practical tool for species identification, when morphological classification of an organism is difficult. Herein we describe the utilisation of this technique in a case of ophthalmomyiasis interna. A 12-year-old boy was infested during a summer holiday in northern Norway, while visiting an area populated with reindeer. Following medical examination, a Diptera larva was surgically removed from the boy’s eye and tentatively identified from its morphological traits as Hypoderma tarandi (L.) (Diptera: Oestridae). Ultimately, DNA barcoding confirmed this impression. The larval cytochrome c oxidase subunit 1 (COI) DNA sequence was matched with both profiles of five adult H. tarandi from the same region where the boy was infested, and other established profiles of H. tarandi in the Barcode of Life Data Systems (BOLD) identification engine.

DNA barcoding of Greenideinae (Hemiptera : Aphididae) with resolving taxonomy problems

2013 ◽  
Vol 27 (4) ◽  
pp. 428 ◽  
Author(s):  
Qing-Hua Liu ◽  
Li-Yun Jiang ◽  
Ge-Xia Qiao
Keyword(s):  
Invasive Species ◽  
Dna Barcoding ◽  
Morphological Traits ◽  
Mitochondrial Gene ◽  
Morphological Evidence ◽  
South East Asia ◽  
Cytb Gene ◽  
Gene Fragment ◽  
Congeneric Species ◽  
Species Circumscription

Species of the Greenideinae are distributed mainly throughout South-east Asia and include some important agricultural and horticultural pests. Rapid and accurate species circumscription and identification in this subfamily are very difficult because similar morphological traits are shared among congeneric species. Here, we test the efficiency of DNA barcoding in the Greenideinae by analysing 214 samples covering 42 species belonging to nine genera using two mitochondrial gene fragments (COI barcode fragment and Cytb gene fragment). The results show that DNA barcoding is a useful species identification method in this subfamily. Both genes can correctly identify most species using neighbour-joining tree analyses and distance-based analyses. Based on the molecular and morphological evidence, we question the validity of two species, Mollitrichosiphum rhusae Ghosh, 1917 and Schoutedenia emblica (Patel & Kulkarni, 1953). Further analysis of the COI barcode fragment shows that Greenidea psidii van der Goot, 1917, an invasive species in Hawaii, is possibly from China. This is a preliminary DNA barcoding study in Greenideinae, and comprehensive sampling is needed to rigorously test the usefulness of DNA barcoding in this subfamily.

Anelaphus villosus (Fabricius) and A. parallelus (Newman) (Coleoptera: Cerambycidae) can be accurately distinguished using body measurements and DNA barcoding

2021 ◽  
pp. 1-13
Author(s):  
William P. Brown ◽  
Robert Salerno ◽  
Luciana Cursino
Keyword(s):  
Natural History ◽  
Dna Barcoding ◽  
Recent Work ◽  
Morphological Traits ◽  
Body Measurements ◽  
Discriminant Functions ◽  
Ecological Understanding ◽  
Longhorned Beetles ◽  
Discriminant Analyses ◽  
Primary Author

Abstract Anelaphus villosus (Fabricius) and A. parallelus (Newman) are longhorned beetles (Coleoptera: Cerambycidae: Cerambycinae: Elaphidiini) that closely resemble each other in appearance. In practice, if antennomere 3 is distinctly longer than antennomere 4, the specimen is considered to be A. villosus, but the accuracy of this methodology is unknown. Authorities disagree about the ease of separating these two species based on morphological traits, and recent work hypothesises that they should be synonymised due to the difficulty of distinguishing them by either morphology or natural history. Assuming correct initial species determinations of curated specimens, as well as of those determined with DNA barcoding and by the primary author, data from 23 body measurements were collected from 50 A. villosus and 60 A. parallelus specimens. Stepwise discriminant analyses and discriminant functions were used to evaluate the ability to distinguish these species based on morphology. Species assignments from discriminant functions were very accurate and were supported by assignments determined with DNA barcoding. The ability to distinguish A. villosus and A. parallelus based on morphological and molecular differences provides evidence against the taxonomic hypothesis of synonymy under one species. A need for greater ecological understanding of these species remains.

DNA barcoding to confirm morphological traits and determine relative abundance of burrowing mayfly species in western Lake Erie

2012 ◽  
Vol 38 (1) ◽  
pp. 180-186 ◽  
Author(s):  
Curt L. Elderkin ◽  
Lynda D. Corkum ◽  
Claudia Bustos ◽  
Erica L. Cunningham ◽  
David J. Berg
Keyword(s):  
Dna Barcoding ◽  
Relative Abundance ◽  
Morphological Traits ◽  
Lake Erie ◽  
Western Lake ◽  
Western Lake Erie ◽  
Burrowing Mayfly

PROFILING OF GUAVA GENOTYPES GROWN IN VIDARBHA CONDITIONS ON THE BASIS OF MORPHOLOGICAL TRAITS

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
R.A. PATIL ◽  
S.G. BHARAD ◽  
S.N. SAWANT
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Morphological Traits ◽  
Considerable Extent ◽  
Fruit Traits ◽  
Breeding Programmes ◽  
Tree Leaf ◽  
Qualitative Characteristics ◽  
Intrapopulation Diversity

Assessment of genetic diversity in the available germplasm is the prerequisite for development of improved genotypes through planned breeding programmes. In the view of this Forty-eight genotypes of seedling origin guava along with 1 check (L-49/Sardar) collected and conserved at germplasm block, Main Garden, Department of Horticulture, Dr. P. D. A. University, Akola were evaluated for genetic variability and diversity based on the qualitative characteristics. The genotypes were evaluated for sixteen morphological traitsviz. tree, leaf, floral and fruit traits. Results Show considerable extent of variability amongst the 49 genotypes in each traits. A sizeable amount of intrapopulation diversity recorded can be used to identify diverse parents which can be utilized in hybridization programmes.

The Medieval Population of Mountainous Ingushetia According to Odontological Data

2019 ◽  
Vol 48 (4) ◽  
pp. 178-196
Author(s):  
Natalya A. Lejbova ◽  
Umalat B. Gadiev
Keyword(s):  
Morphological Traits ◽  
Dental Anthropology ◽  
Deep Roots ◽  
The Caucasus ◽  
New Material ◽  
The Republic ◽  
The Village ◽  
Medieval Population

Although population of the Caucasus has been studied in a rather detailed way, there are peoples whose anthropological portrait is still incomplete. Among them are the Ingush, one of the oldest autochthonous peoples of the Caucasus. This work presents new material on the dental anthropology of medieval Ingush, collected in 2017 during expeditions to the Jairakh and Sunzhen districts of the Republic of Ingushetia. In the Jairakh district, the investigations were carried out in the crypt complexes of the 15th–18th centuries – Targim, Agikal, Tsori, Salgi, and in Sunzhen region - in crypts near the village of Muzgan. The craniological series of medieval Ingush studied according to the dental anthropology program can be described as belonging to the western range of odontological complexes. Unlike most modern Caucasian groups, it does not belong to gracile forms, but rather to a maturized odontological variant, which has deep roots in the Caucasus. The results once again demonstrate a certain conservatism and stability of the dental system, which preserves morphological traits of ancestral groups longer than other anthropological systems.

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