scholarly journals Expression of cell-adhesion molecules in embryonic induction. I. Morphogenesis of nestling feathers.

1985 ◽  
Vol 101 (3) ◽  
pp. 1009-1026 ◽  
Author(s):  
C M Chuong ◽  
G M Edelman
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
General Pattern ◽  
Immunohistochemical Analysis ◽  
Dermal Papilla ◽  
Cross Reactivity ◽  
Basement Membranes ◽  
Embryonic Induction ◽  
Feather Follicle

The potential relationship of cell adhesion to embryonic induction during feather formation was examined by immunohistochemical analysis of the spatiotemporal distribution of three cell-adhesion molecules (CAMs), neural CAM (N-CAM), liver CAM (L-CAM), and neuron-glia CAM (Ng-CAM), and of substrate molecules (laminin and fibronectin) in embryonic chicken skin. The N-CAM found at sites of embryonic induction in the feather was found to be similar to brain N-CAM as judged by immuno-cross-reactivity, migratory position in PAGE, and the presence of embryonic to adult conversion. In contrast to the N-CAM found in the brain, however, only one polypeptide of Mr 140,000 was seen. N-CAM-positive dermal condensations were distributed periodically under L-CAM-positive feather placodes at those sites where basement membranes are known to be disrupted. After initiation of induction, L-CAM-positive placode cells became transiently N-CAM-positive. N-CAM was asymmetrically concentrated in the dorsal region of the feather bud, while fibronectin was concentrated in the ventral region. During feather follicle formation, N-CAM was expressed in the dermal papilla and was closely apposed to the L-CAM-positive papillar ectoderm, while the dermal papilla showed no evidence of laminin or fibronectin. The collar epithelium was both N-CAM- and L-CAM-positive. During the formation of the feather filament, N-CAM appeared periodically and asymmetrically on basilar cells located in the valleys between adjacent barb ridges. In contrast to the two primary CAMs, Ng-CAM was found only on nerves supplying the feather and the skin. These studies indicate that at each site of induction during feather morphogenesis, a general pattern is repeated in which an epithelial structure linked by L-CAM is confronted with periodically propagating condensations of cells linked by N-CAM.

Neural cell adhesion molecules during embryonic induction and development of the kidney

Development ◽  
1988 ◽  
Vol 102 (4) ◽  
pp. 749-761 ◽  
Author(s):  
G. Klein ◽  
M. Langegger ◽  
C. Goridis ◽  
P. Ekblom
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Kidney Development ◽  
Neural Cell ◽  
Cell Polarization ◽  
Embryonic Induction ◽  
Neural Cell Adhesion Molecules ◽  
Short Period ◽  
Neural Cell Adhesion

The neural cell adhesion molecules (N-CAM) are a family of related glycoproteins with Mr of 180, 140 and 120 × 10(3) (180K etc.). In the embryo, they are often highly sialylated and migrate as a diffuse band of 170–250K. N-CAM are found in non-neural tissues and we have now studied the expression of N-CAM in the developing mouse kidney. During kidney development, a unique conversion of a mesenchyme to an epithelium occurs and it is thought that this is mediated by an increase in cell adhesivity. By immunofluorescence, we show that N-CAM is present already at onset of kidney development on the cells of the uninduced nephrogenic mesenchyme. After induction, when the cells convert into an epithelium, they lose N-CAM gradually and instead begin to express uvomorulin, another primary CAM. By using an organ culture model, we could rather precisely show that N-CAM and uvomorulin are coexpressed for a short period, but, when epithelial cell polarization is evident, only uvomorulin is present on the epithelium, whereas N-CAM is confined to the surrounding mesenchyme. Immunoblotting for N-CAM revealed that the ‘embryonic’ form of N-CAM, the broad 170–250K band was not present in the embryonic kidney, which instead expressed the three distinct 180K, 140K and 120K bands typical of adult neurones. The 180K and 140K bands were gradually lost during development and were no longer detectable in adult kidneys. By using an N-CAM cDNA, we detected three different mRNAs of 7.4, 6.7 and 4.3 kb in the developing kidney, but this expression was restricted to the embryonic and early postnatal stages. No transcripts were detectable in adult kidneys. The studies do not support the hypothesis that N-CAM expression in the kidney is turned on by embryonic induction. Rather, we suggest that N-CAM are important adhesives for the predetermined, but not yet induced, nephrogenic mesenchyme.

scholarly journals Expression of cell-adhesion molecules in embryonic induction. II. Morphogenesis of adult feathers.

1985 ◽  
Vol 101 (3) ◽  
pp. 1027-1043 ◽  
Author(s):  
C M Chuong ◽  
G M Edelman
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Staining Pattern ◽  
Dermal Papilla ◽  
Morphological Transformation ◽  
Adult Chicken ◽  
Chicken Tissue ◽  
Chicken Skin ◽  
Cyclic Expression

The developmental appearance of cell-adhesion molecules (CAMs) was mapped during the morphogenesis of the adult chicken feather. Neural CAM (N-CAM), liver CAM (L-CAM), and neuron-glia CAM (Ng-CAM), as well as substrate molecules (laminin and fibronectin), were compared in newborn chicken skin by immunohistochemical means. N-CAM was found to be enriched in the dermal papilla, which was closely apposed to L-CAM-positive papillar ectoderm. The two CAMs were then co-expressed in cells of the collar epithelium. Subsequently generated barb epithelia expressed only L-CAM, but N-CAM reappeared periodically on cells between developing barbs and barbules. N-CAM first appeared on a single L-CAM-positive basilar cell located in each valley flanked by two adjacent barb ridges. Subsequently, the expression of N-CAM extended one cell after another to include the whole basilar layer. N-CAM also appeared in the L-CAM-positive axial-plate epithelia, beginning in a single cell located at the ridge base. The two collectives of N-CAM-positive epithelia constituting the marginal and axial plates then disintegrated, leaving interdigitating spaces between keratinized structures that had previously expressed L-CAM. The morphological transformation from an epithelial cylinder to a three-level branched feather pattern is thus achieved by coupling alternating CAM expression in linked cell collectives with specific differentiation events, such as keratinization. During all of these morphogenetic processes, laminin and fibronectin formed a continuous basement membrane separating pulp from feather epithelia, and were excluded from the sites involved in periodic appearances of N-CAM. The same staining pattern described for developing chickens persisted in the feather follicles of adult chicken tissue that have gone through several cycles of molting. Cyclic expression of the two different CAMs underlies each of the different morphological events that are generated epigenetically during feather morphogenesis.

scholarly journals Expression of Three Cell Adhesion Molecules in Bladder Carcinomas: Correlation with Pathological Features

1997 ◽  
Vol 13 (3) ◽  
pp. 125-136 ◽  
Author(s):  
Agnès Mialhe ◽  
Josette Louis ◽  
Dominique Pasquier ◽  
Jean‐Jacques Rambeaud ◽  
Daniel Seigneurin
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Tumour Progression ◽  
Histological Grade ◽  
Immunohistochemical Analysis ◽  
Aberrant Expression ◽  
Bladder Carcinomas ◽  
Bladder Tumours ◽  
E Cadherin

Recently, independent studies have shown that the expression of two integrin chains,β4 andα2, plus the epithelial cadherin are related to tumour progression in human bladder carcinomas. For the first time, we compare the expression of these three cell adhesion molecules using immunohistochemical analysis of consecutive cryosections from a series of 50 bladder tumours. E‐cadherin,β4, andα2 were strongly expressed in normal urothelium. A majority of non‐invasive bladder cancers stained positively for E‐cadherin (62%), whereas only 29% expressed normal positivity forα2, and only 35% forβ4. However, most invasive tumours presented an aberrant expression ofα2 (81%),β4 (100%), and E‐cadherin (75%). We studied the correlation of immunoreactivity with histological grade and stage. Theα2 pattern was not correlated with stage and grade. In contrast, loss of normalβ4 expression was significantly related to increasing tumour grade and deep invasion with a higher correlation for grade. Finally, E‐cadherin expression was highly correlated with stage, but not with grade. Thus our results indicate that, although many invasive bladder tumours presented a disorder in expression of the two integrinsα2 andβ4, E‐cadherin appeared to be a better marker of invasiveness in bladder carcinomas.

Expression of cell adhesion molecules during embryonic induction

1987 ◽  
Vol 119 (1) ◽  
pp. 217-230 ◽  
Author(s):  
Guy P. Richardson ◽  
Kathryn L. Crossin ◽  
Cheng-Ming Chuong ◽  
Gerald M. Edelman
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Embryonic Induction

scholarly journals Carcinosarcoma of the parotid gland: Immunohistochemical analysis with emphasis in cell cycle mitotic activity and cell adhesion molecules expression

2006 ◽  
Vol 42 (4) ◽  
pp. 140-143 ◽  
Author(s):  
Dimitrios Andreadis ◽  
Athanasios Poulopoulos ◽  
Apostolos Epivatianos ◽  
Alexandros Nomikos ◽  
Konstantinos Christidis ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Adhesion ◽  
Parotid Gland ◽  
Mitotic Activity ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Immunohistochemical Analysis
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