scholarly journals Monoclonal antibodies as probes of epithelial membrane polarization.

1985 ◽  
Vol 101 (6) ◽  
pp. 2173-2180 ◽  
Author(s):  
R J Turner ◽  
J Thompson ◽  
S Sariban-Sohraby ◽  
J S Handler
Keyword(s):  
Monoclonal Antibodies ◽  
Plasma Membrane ◽  
Membrane Lipids ◽  
Apical Plasma Membrane ◽  
Epithelial Cell Line ◽  
Lipid Species ◽  
Antigen Complex ◽  
Kidney Epithelial Cell ◽  
Kidney Epithelial Cell Line ◽  
Lipid Antigen

Monoclonal antibodies directed against antigens in the apical plasma membrane of the toad kidney epithelial cell line A6 were produced to probe the phenomena that underlie the genesis and maintenance of epithelial polarity. Two of these antibodies, 17D7 and 18C3, were selected for detailed study here. 17D7 is directed against a 23-kD peptide found on both the apical and basolateral surfaces of the A6 epithelium whereas 18C3 recognizes a lipid localized to the apical membrane only. This novel observation of an apically localized epithelial lipid species indicates the existence of a specific sorting and insertion process for this, and perhaps other, epithelial plasma membrane lipids. The antibody-antigen complexes formed by both these monoclonal antibodies are rapidly internalized by the A6 cells, but only the 18C3-antigen complex is recycled to the plasma membrane. In contrast to the apical localization of the free antigen, however, the 18C3-antigen complex is recycled to both the apical and basolateral surface of the epithelium, which indicates that monoclonal antibody binding interferes in some way with the normal sorting process for this apical lipid antigen.

scholarly journals Interaction of Azole Antifungal Agents with Human P-glycoprotein Expressed in a Kidney Epithelial Cell Line, LLC-PK1.

2001 ◽  
Vol 16 (1) ◽  
pp. 5-11
Author(s):  
Takaaki KODAWARA ◽  
Ikuko YANO ◽  
Satohiro MASUDA ◽  
Tatsuya ITO ◽  
Hiroko WAKASUGI ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Antifungal Agents ◽  
Epithelial Cell Line ◽  
P Glycoprotein ◽  
Kidney Epithelial Cell ◽  
Kidney Epithelial Cell Line

scholarly journals Criticality of plasma membrane lipids reflects activation state of macrophage cells

2020 ◽  
Vol 17 (163) ◽  
pp. 20190803 ◽  
Author(s):  
Eugenia Cammarota ◽  
Chiara Soriani ◽  
Raphaelle Taub ◽  
Fiona Morgan ◽  
Jiro Sakai ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Critical Point ◽  
Membrane Lipids ◽  
Membrane Receptors ◽  
Interleukin 4 ◽  
Critical Conditions ◽  
Membrane Composition ◽  
Macrophage Cells ◽  
Lipid Species ◽  
Anti Inflammatory

Signalling is of particular importance in immune cells, and upstream in the signalling pathway many membrane receptors are functional only as complexes, co-locating with particular lipid species. Work over the last 15 years has shown that plasma membrane lipid composition is close to a critical point of phase separation, with evidence that cells adapt their composition in ways that alter the proximity to this thermodynamic point. Macrophage cells are a key component of the innate immune system, are responsive to infections and regulate the local state of inflammation. We investigate changes in the plasma membrane’s proximity to the critical point as a response to stimulation by various pro- and anti-inflammatory agents. Pro-inflammatory (interferon γ , Kdo 2-Lipid A, lipopolysaccharide) perturbations induce an increase in the transition temperature of giant plasma membrane vesicles; anti-inflammatory interleukin 4 has the opposite effect. These changes recapitulate complex plasma membrane composition changes, and are consistent with lipid criticality playing a master regulatory role: being closer to critical conditions increases membrane protein activity.

Transcellular transport of 4-iodo-L-meta-tyrosine via system L across monolayers of kidney epithelial cell line LLC-PK1

2004 ◽  
Vol 31 (4) ◽  
pp. 477-482 ◽  
Author(s):  
Naoto Shikano ◽  
Keiichi Kawai ◽  
Syuichi Nakajima ◽  
Akiko Kubodera ◽  
Nobuo Kubota ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Transcellular Transport ◽  
System L ◽  
Kidney Epithelial Cell ◽  
Kidney Epithelial Cell Line

Transcellular transport of radioiodinated 3-iodo-α-methyl-L-tyrosine across monolayers of kidney epithelial cell line LLC-PK1

2004 ◽  
Vol 18 (3) ◽  
pp. 227-234 ◽  
Author(s):  
Naoto Shikano ◽  
Keiichi Kawai ◽  
Syuichi Nakajima ◽  
Akiko Kubodera ◽  
Nobuo Kubota ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Transcellular Transport ◽  
Kidney Epithelial Cell ◽  
Kidney Epithelial Cell Line

scholarly journals Synthesis and Cytotoxicity Studies of Titanocene C Analogues

2008 ◽  
Vol 2008 ◽  
pp. 1-7 ◽  
Author(s):  
Megan Hogan ◽  
James Claffey ◽  
Eoin Fitzpatrick ◽  
Thomas Hickey ◽  
Clara Pampillón ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Pig Kidney ◽  
Cytotoxicity Studies ◽  
Kidney Epithelial Cell ◽  
Kidney Epithelial Cell Line

From the carbolithiation of 6-N,N-dimethylamino fulvene (3) and 2,4[bis(N,N-dimethylamino)methyl]-N-methylpyrrolyl lithium (2a), N-(N′,N′-dimethylaminomethyl)benzimidazolyl lithium (2b)' or p-(N,N-dimethylamino)methylphenyl lithium (2c), the corresponding lithium cyclopentadienide intermediate (4a–c) was formed. These three lithiated intermediates underwent a transmetallation reaction with TiCl4' resulting in N,N-dimethylamino-functionalised titanocenes 5a–c. When these titanocenes were tested against a pig kidney epithelial cell line (LLC-PK), the IC50 values obtained were of 23, and 52 μM for titanocenes 5a and 5b, respectively. The most cytotoxic titanocene in this paper, 5c with an IC50 value of 13 μM, was found to be approximately two times less cytotoxic than its analogue Titanocene C (IC50=5.5 μM) and almost four times less cytotoxic than cisplatin, which showed an IC50 value of 3.3 μM when tested on the LLC-PK cell line.

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