scholarly journals Localization of phycoerythrin at the lumenal surface of the thylakoid membrane in Rhodomonas lens.

1989 ◽  
Vol 108 (3) ◽  
pp. 875-884 ◽  
Author(s):  
M Ludwig ◽  
S P Gibbs
Keyword(s):  
New York ◽  
Thylakoid Membrane ◽  
Close Association ◽  
New York State ◽  
Protein A ◽  
York State Department ◽  
Gold Particles ◽  
Whole Cells ◽  
Dense Substance

The thylakoids of cryptomonads are unique in that their lumens are filled with an electron-dense substance postulated to be phycobiliprotein. In this study, we used an antiserum against phycoerythrin (PE) 545 of Rhodomonas lens (gift of R. MacColl, New York State Department of Health, Albany, NY) and protein A-gold immunoelectron microscopy to localize this light-harvesting protein in cryptomonad cells. In sections of whole cells of R. lens labeled with anti-PE 545, the gold particles were not uniformly distributed over the dense thylakoid lumens as expected, but instead were preferentially localized either over or adjacent to the thylakoid membranes. A similar pattern of labeling was observed in cell sections labeled with two different antisera against PE 566 from Cryptomonas ovata. To determine whether PE is localized on the outer or inner side of the membrane, chloroplast fragments were isolated from cells fixed in dilute glutaraldehyde and labeled in vitro with anti-PE 545 followed by protein A-small gold. These thylakoid preparations were then fixed in glutaraldehyde followed by osmium tetroxide, embedded in Spurr, and sections were labeled with anti-PE 545 followed by protein A-large gold. Small gold particles were found only at the broken edges of the thylakoids, associated with the dense material on the lumenal surface of the membrane, whereas large gold particles were distributed along the entire length of the thylakoid membrane. We conclude that PE is located inside the thylakoids of R. lens in close association with the lumenal surface of the thylakoid membrane.

New Paradigm for State Department of Transportation Environmental Initiatives: New York State’s Guidelines and Procedures

2000 ◽  
Vol 1702 (1) ◽  
pp. 92-96 ◽  
Author(s):  
Gary McVoy ◽  
Mark Sengenberger ◽  
Elizabeth Novak
Keyword(s):  
New York ◽  
New York State ◽  
Cost Effective ◽  
State Department ◽  
Regulatory Compliance ◽  
Environmental Benefits ◽  
Environmental Damage ◽  
York State Department ◽  
Department Of Transportation ◽  
New Paradigm

Public-works agencies have an obligation to enhance the environment as opportunities arise. The New York State Department of Transportation (NYSDOT) has developed an environmental initiative to make an affirmative contribution to the environment, using the department’s organizational strengths. The environmental initiative is a paradigm shift applicable to all departments of transportation (DOTs). Conventional reactive regulatory compliance can reduce unnecessary environmental damage and sometimes gain grudging regulatory agency cooperation; however, it is not a positive, satisfying way of doing the people’s work. Through proactive steps, NYSDOT has become an important part of the state’s environmental solution (often at little or no additional cost) and has changed its working relationships with environmental agencies and groups. As these agencies and groups have become partners, instead of adversaries, permit-approval times have improved, mitigation costs have declined, morale has improved, and cost-effective environmental benefits are being realized. Procedures are outlined to apply the engineering capabilities of a DOT to the environmental-stewardship responsibilities shared by all governmental organizations.

scholarly journals Response of an Obstetric Unit during the Coronavirus Disease of 2019 (COVID-19) Pandemic: Experiences from a Tertiary Care Center

2020 ◽  
Vol 10 (03) ◽  
pp. e281-e287
Author(s):  
Sangeeta Kumaraswami ◽  
Tana S. Pradhan ◽  
Sorana Vrabie-Wolf ◽  
Sadaf Lodhi ◽  
Geetha P. Rajendran ◽  
...  
Keyword(s):  
New York ◽  
Care Center ◽  
Tertiary Care ◽  
New York State ◽  
York State Department ◽  
Obstetrical Care ◽  
Expert Opinions ◽  
Department Of Health ◽  
Delivery Unit ◽  
Westchester County

Abstract Objective To describe our experiences in preparing our obstetric unit in Westchester County, New York, during the COVID-19 (coronavirus disease of 2019) pandemic. We focus on describing our timeline, continuously evolving actions, observations, and challenges. Methods With guidance from the New York State Department of Health (NYSDOH), our institutional epidemiologist, and key multidisciplinary faculty members, we evaluated emerging national data as well as expert opinions to identify issues and challenges to create action plans. Results We created and modified policies for our patients presenting for obstetrical care on the labor and delivery unit to accommodate their unique needs during this pandemic. Conclusion The COVID-19 pandemic has posed many unique challenges. Balancing communication, risks of infection to providers, patient autonomy and rights, and resources for testing and personal protective equipment were among the valuable lessons learnt. We have shared our experiences and described our observations and challenges in Westchester County, New York.

Alcohol Related Birth Defects: Implications for Education

1982 ◽  
Vol 12 (2) ◽  
pp. 113-123 ◽  
Author(s):  
Michael Lamanna
Keyword(s):  
New York ◽  
Birth Defects ◽  
School Adjustment ◽  
New York State ◽  
Department Of Education ◽  
York State Department ◽  
Preventive Action ◽  
Fetal Alcohol ◽  
Behavioral Learning ◽  
Community Agencies

This article emerges from a larger study of preventive approaches for Alcohol Related Birth Defects (ARBD) prepared for the New York State Department of Education. The author contends that in addition to clearly identifiable Fetal Alcohol Syndrome children, there is a continuum of impairment to the offspring of drinking mothers that is dose related and produces serious behavioral/learning deficits. The continuum includes young people of normal intelligence who perform below expected levels and find school adjustment difficult. School and community agencies need to conduct studies to determine the nature and extent of impairment and the kind of interventive and preventive action that should be instituted. To that end, this article gives information on the background and nature of ARBD and some suggestions to guide development of programs.

Simple Model of Forward Visibility for Snowplow Operators Through Snow and Fog at Night

1997 ◽  
Vol 1585 (1) ◽  
pp. 19-24 ◽  
Author(s):  
John Bullough ◽  
Mark S. Rea
Keyword(s):  
New York ◽  
New York State ◽  
Field Tests ◽  
Line Of Sight ◽  
York State Department ◽  
Light Sources ◽  
Department Of Transportation ◽  
Narrow Beam ◽  
York State ◽  
Beam Type

Backscattered light reflected from falling snow, rain, and fog impairs visibility and can hinder performance of snowplow operators. New York State Department of Transportation snowplow operators cite backscattered glare as an important problem, and many drivers use their own “solutions” to counteract it. A literature review yielded two important conclusions: backscattered light from a lamp in fog and falling snow is reduced, and visibility is improved ( a) as the lamp is moved away from the operator’s line of sight and ( b) with a narrow-beam lamp. Field tests of lighting configurations during inclement weather were conducted to determine the effects of beam type and displacement from the operator’s line of sight on visibility, glare, and satisfaction. On the basis of these data and the literature, a simple mathematical model was developed that predicts the forward-visibility ratings of snowplow operators. This model can be used to guide specifiers in selecting light sources and mounting locations for snowplow operations.

scholarly journals Impatiens necrotic spot virus in Greenhouse-Grown Potatoes in New York State

Plant Disease ◽  
2005 ◽  
Vol 89 (3) ◽  
pp. 340-340 ◽  
Author(s):  
K. L. Perry ◽  
L. Miller ◽  
L. Williams
Keyword(s):  
New York ◽  
Tomato Spotted Wilt Virus ◽  
New York State ◽  
Impatiens Necrotic Spot Virus ◽  
Spot Virus ◽  
York State ◽  
Tomato Spotted Wilt ◽  
In Vitro Plantlets ◽  
Wilt Virus

Impatiens necrotic spot virus (INSV; genus Tospovirus) was detected in experimental greenhouse-grown potatoes (Solanum tuberosum) and Nicotiana benthamiana in New York State in July and August of 2003 and 2004. Potato leaves exhibiting necrotic lesions with a concentric pattern similar to those induced by Tomato spotted wilt virus (1) were observed on cvs. Atlantic, Huckleberry, NY115, and Pentland Ivory. The presence of INSV was confirmed using double-antibody sandwich enzyme-linked immunosorbent assay and a rapid ‘ImmunoStrip’ assay (Agdia, Inc., Elkhart, IN). INSV-specific sequences were amplified from total RNA extracts using reverse transcription-polymerase chain reaction with ‘Tospovirus Group’ primers (Agdia, Inc.) and two independently amplified DNAs were sequenced. A common sequence of 355 nucleotides (GenBank Accession No. AY775324) showed 98% identity to coding sequences in an INSV L RNA. The virus was mechanically transmitted to potato and N. benthamiana and could be detected in asymptomatic, systemically infected potato leaves. Stems nodes and leaves were removed from infected potato plants, and sterile in vitro plantlets were established (2). None of the regenerated in vitro plantlets of cvs. Pentland Ivory (6 plantlets) or NY115 (5 plantlets) were infected with INSV. Two of ten regenerated cv. Atlantic plantlets initially tested positive, but INSV could not be detected after 6 months in tissue culture. In vitro tissue culture plantlets could not be established from infected cv. Huckleberry plants, even though they were consistently obtained from uninfected plants. Infected greenhouse plants were grown to maturity and the tubers harvested, stored for 6 months at 4°C, and replanted in the greenhouse. INSV could not be detected in plants from 26 cv. Huckleberry, 4 cv. NY115, or 4 cv. Atlantic tubers. Although this isolate of INSV was able to systemically infect potato, it was not efficiently maintained or transmitted to progeny tubers. This might explain why INSV has not been reported as a problem in potato production. Lastly, in both years, dying N. benthamiana provided the first sign of a widespread greenhouse infestation of INSV in a university facility housing ornamental and crop plants. INSV induced a systemic necrosis in N. benthamiana, and this host may be useful as a sensitive ‘trap’ plant indicator for natural infections in greenhouse production. References: (1) T. L. German. Tomato spotted wilt virus. Pages 72–73 in: Compendium of Potato Diseases. W. R. Stevenson et al., eds. The American Phytopathological Society, St. Paul, 2001. (2) S. A. Slack and L. A. Tufford. Meristem culture for virus elimination. Pages 117–128 in: Fundamental Methods of Plant Cell, Tissue and Organ Culture and Laboratory Operations. O. L. Gamborg and G. C. Philips, eds. Springer-Velag, Berlin, 1995.

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