scholarly journals Robust polarity establishment occurs via an endocytosis-based cortical corralling mechanism

2013 ◽  
Vol 200 (4) ◽  
pp. 407-418 ◽  
Author(s):  
Mini Jose ◽  
Sylvain Tollis ◽  
Deepak Nair ◽  
Jean-Baptiste Sibarita ◽  
Derek McCusker
Keyword(s):  
High Resolution ◽  
Membrane Trafficking ◽  
In Vivo Studies ◽  
Biological Processes ◽  
Constant Frequency ◽  
In Silico Modeling ◽  
Resolution Imaging ◽  
Polarity Establishment ◽  
Endocytic Vesicles

Formation of a stable polarity axis underlies numerous biological processes. Here, using high-resolution imaging and complementary mathematical modeling we find that cell polarity can be established via the spatial coordination of opposing membrane trafficking activities: endocytosis and exocytosis. During polarity establishment in budding yeast, these antagonistic processes become apposed. Endocytic vesicles corral a central exocytic zone, tightening it to a vertex that establishes the polarity axis for the ensuing cell cycle. Concomitantly, the endocytic system reaches an equilibrium where internalization events occur at a constant frequency. Endocytic mutants that failed to initiate periodic internalization events within the corral displayed wide, unstable polarity axes. These results, predicted by in silico modeling and verified by high resolution in vivo studies, identify a requirement for endocytic corralling during robust polarity establishment.

scholarly journals Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Philipp S. Lienemann ◽  
Stéphanie Metzger ◽  
Anna-Sofia Kiveliö ◽  
Alain Blanc ◽  
Panagiota Papageorgiou ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Biological Processes ◽  
Micro Ct ◽  
Pinhole Spect

Abstract Over the last decades, great strides were made in the development of novel implants for the treatment of bone defects. The increasing versatility and complexity of these implant designs request for concurrent advances in means to assess in vivo the course of induced bone formation in preclinical models. Since its discovery, micro-computed tomography (micro-CT) has excelled as powerful high-resolution technique for non-invasive assessment of newly formed bone tissue. However, micro-CT fails to provide spatiotemporal information on biological processes ongoing during bone regeneration. Conversely, due to the versatile applicability and cost-effectiveness, single photon emission computed tomography (SPECT) would be an ideal technique for assessing such biological processes with high sensitivity and for nuclear imaging comparably high resolution (<1 mm). Herein, we employ modular designed poly(ethylene glycol)-based hydrogels that release bone morphogenetic protein to guide the healing of critical sized calvarial bone defects. By combined in vivo longitudinal multi-pinhole SPECT and micro-CT evaluations we determine the spatiotemporal course of bone formation and remodeling within this synthetic hydrogel implant. End point evaluations by high resolution micro-CT and histological evaluation confirm the value of this approach to follow and optimize bone-inducing biomaterials.

Zn-doping enhances the photoluminescence and stability of PbS quantum dots for in vivo high-resolution imaging in the NIR-II window

Nano Research ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 2239-2245 ◽  
Author(s):  
Xiulei Shi ◽  
Song Chen ◽  
Meng-Yao Luo ◽  
Biao Huang ◽  
Guozhen Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
High Resolution ◽  
High Resolution Imaging ◽  
Zn Doping ◽  
Resolution Imaging ◽  
Pbs Quantum Dots

scholarly journals Hemoglobin Video Imaging Provides Novel In Vivo High-Resolution Imaging and Quantification of Human Aqueous Outflow in Patients with Glaucoma

2019 ◽  
Vol 2 (5) ◽  
pp. 327-335 ◽  
Author(s):  
Tasneem Z. Khatib ◽  
Paul A.R. Meyer ◽  
Jed Lusthaus ◽  
Ilya Manyakin ◽  
Yusuf Mushtaq ◽  
...  
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
Video Imaging ◽  
Resolution Imaging ◽  
Aqueous Outflow

scholarly journals Method for high-resolution imaging of creatine in vivo using chemical exchange saturation transfer

2013 ◽  
Vol 71 (1) ◽  
pp. 164-172 ◽  
Author(s):  
Feliks Kogan ◽  
Mohammad Haris ◽  
Anup Singh ◽  
Kejia Cai ◽  
Catherine Debrosse ◽  
...  
Keyword(s):  
High Resolution ◽  
Chemical Exchange ◽  
Chemical Exchange Saturation Transfer ◽  
High Resolution Imaging ◽  
Saturation Transfer ◽  
Resolution Imaging

High-Resolution Imaging of the Human Retina In Vivo after Scatter Photocoagulation Treatment Using a Semiautomated Laser System

Ophthalmology ◽  
2010 ◽  
Vol 117 (3) ◽  
pp. 545-551 ◽  
Author(s):  
Katharina Kriechbaum ◽  
Matthias Bolz ◽  
Gabor G. Deak ◽  
Sonja Prager ◽  
Christoph Scholda ◽  
...  
Keyword(s):  
High Resolution ◽  
Laser System ◽  
High Resolution Imaging ◽  
Human Retina ◽  
Photocoagulation Treatment ◽  
Resolution Imaging

scholarly journals Live-cell vibrational imaging of choline metabolites by stimulated Raman scattering coupled with isotope-based metabolic labeling

The Analyst ◽  
2014 ◽  
Vol 139 (10) ◽  
pp. 2312-2317 ◽  
Author(s):  
Fanghao Hu ◽  
Lu Wei ◽  
Chaogu Zheng ◽  
Yihui Shen ◽  
Wei Min
Keyword(s):  
High Resolution ◽  
Raman Scattering ◽  
Mammalian Cells ◽  
Stimulated Raman Scattering ◽  
Primary Neurons ◽  
High Resolution Imaging ◽  
Metabolic Labeling ◽  
C Elegans ◽  
Resolution Imaging

High-resolution imaging of choline metabolites in living mammalian cells, primary neurons andC. eleganshas been demonstrated with the potential forin vivodisease detection and developmental monitoring.

Long-Term, High-Resolution Imaging in the Neocortex In Vivo

2008 ◽  
Vol 2008 (2) ◽  
pp. pdb.prot4902-pdb.prot4902 ◽  
Author(s):  
B. E. Chen ◽  
J. T. Trachtenberg ◽  
A. J.G.D. Holtmaat ◽  
K. Svoboda
Keyword(s):  
High Resolution ◽  
High Resolution Imaging ◽  
Resolution Imaging
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