scholarly journals Localization of vicilin peptidohydrolase in the cotyledons of mung bean seedlings by immunofluorescence microscopy.

1978 ◽  
Vol 79 (1) ◽  
pp. 10-19 ◽  
Author(s):  
B Baumgartner ◽  
K T Tokuyasu ◽  
M J Chrispeels
Keyword(s):  
Vigna Radiata ◽  
Mung Bean ◽  
Immunofluorescence Microscopy ◽  
De Novo ◽  
Protein Bodies ◽  
Vascular Bundles ◽  
Large Protein ◽  
Parenchyma Cells ◽  
Storage Parenchyma ◽  
Monospecific Antibodies

Vicilin peptidohydrolase, the protease that hydrolyzes the reserve proteins in the cotyledons of mung bean (Vigna radiata) seedlings, has been localized intracellularly by immunofluorescence microscopy using monospecific antibodies against the enzyme and rhodamine-coupled goat-anti-rabbit immunoglobulin G's. The enzyme can first be visualized after 3 days of seedling growth and is associated with small foci within the cytoplasm of the storage parenchyma cells farthest from the vascular bundles. On the 4th day of growth, the protease is also present in the numerous large protein bodies within these cells. Vicilin peptidohydrolase is known to be synthesized de novo starting on the 3rd day of growth. Our observations are therefore consistent with the interpretation that the enzyme is synthesized in the cytoplasm and subsequently transported to the protein bodies.

Cellular pathways of source leaf phloem loading and phloem unloading in developing stems of Sorghum bicolor in relation to stem sucrose storage

2015 ◽  
Vol 42 (10) ◽  
pp. 957 ◽  
Author(s):  
Ricky J. Milne ◽  
Christina E. Offler ◽  
John W. Patrick ◽  
Christopher P. L. Grof
Keyword(s):  
Sorghum Bicolor ◽  
Cell Walls ◽  
Phloem Loading ◽  
Vascular Bundles ◽  
Phloem Unloading ◽  
Parenchyma Cells ◽  
Storage Parenchyma ◽  
Sucrose Storage ◽  
Cellular Pathways ◽  
And Storage

Cellular pathways of phloem loading in source leaves and phloem unloading in stems of sweet Sorghum bicolor (L.) Moench were deduced from histochemical determinations of cell wall composition and from the relative radial mobilities of fluorescent tracer dyes exiting vascular pipelines. The cell walls of small vascular bundles in source leaves, the predicted site of phloem loading, contained minimal quantities of lignin and suberin. A phloem-loaded symplasmic tracer, carboxyfluorescein, was retained within the collection phloem, indicating symplasmic isolation. Together, these findings suggested that phloem loading in source leaves occurs apoplasmically. Lignin was restricted to the walls of protoxylem elements located in meristematic, elongating and recently elongated regions of the stem. The apoplasmic tracer, 8-hydroxypyrene-1,3,6-trisulfonic acid, moved radially from the transpiration stream, consistent with phloem and storage parenchyma cells being interconnected by an apoplasmic pathway. The major phase of sucrose accumulation in mature stems coincided with heavy lignification and suberisation of sclerenchyma sheath cell walls restricting apoplasmic tracer movement from the phloem to storage parenchyma apoplasms. Phloem unloading at this stage of stem development followed a symplasmic route linking sieve elements and storage parenchyma cells, as confirmed by the phloem-delivered symplasmic tracer, 8-hydroxypyrene-1,3,6-trisulfonic acid, moving radially from the stem phloem.

Temporal and spatial expression of hexose transporters in developing tomato (Lycopersicon esculentum) fruit

2005 ◽  
Vol 32 (9) ◽  
pp. 777 ◽  
Author(s):  
Stephen J. Dibley ◽  
Michael L. Gear ◽  
Xiao Yang ◽  
Elke G. Rosche ◽  
Christina E. Offler ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Fruit Ripening ◽  
Tomato Fruit ◽  
Vascular Bundles ◽  
Hexose Transporter ◽  
Transporter Protein ◽  
Protein Levels ◽  
Parenchyma Cells ◽  
Storage Parenchyma ◽  
Post Transcriptional Regulation

Correlative physiological evidence suggests that membrane transport into storage parenchyma cells is a key step in determining hexose levels accumulated in tomato (Lycopersicon esculentum Mill.) fruit (Ruan et al. 1997). Expression of three previously identified hexose transporter genes (LeHT1, 2 and 3) demonstrated that LeHT3, and to a lesser extent LeHT1, are the predominant transporters expressed in young fruit (10 d after anthesis; DAA). Expression of both transporters dropped sharply until 24 DAA, after which only LeHT3 expression remained at detectable levels through to fruit ripening. LeHT2 was not expressed substantially until the onset of fruit ripening. For fruit at both 10 and 30 DAA, LeHT3 transcripts were detected in storage parenchyma cells of the outer pericarp tissue, but not in vascular bundles or the first layer of parenchyma cells surrounding these bundles. In contrast to LeHT gene expression, hexose transporter protein levels were maximal between 20 and 30 DAA, which corresponded to the period of highest hexose accumulation. The delayed appearance of transporter protein is consistent with some form of post-transcriptional regulation. Based on these analyses, LeHT3 appears to be responsible for the rapid hexose accumulation in developing tomato fruit.

Localization of amylase activity in cotyledons of germinated mung bean seeds

1991 ◽  
Vol 69 (7) ◽  
pp. 1501-1506 ◽  
Author(s):  
Yasuko Kaneko ◽  
Hisashi Matsushima ◽  
Yukio Morohashi
Keyword(s):  
Key Words ◽  
Seed Coat ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Amylase Activity ◽  
The Other ◽  
Vascular Bundles ◽  
Starch Granules ◽  
Developmental Patterns ◽  
Cell Layers

Developmental patterns of amylase activity in mung bean (Vigna radiata) cotyledons were observed by histochemical methods. In axis-attached cotyledons, the development of amylase activity and the decompostion of starch grains occurred first in cells located farthest from vascular bundles, and 3 days after imbibition, starch granules appeared to remain intact only in two to three cell layers around the vascular bundles. In axis-detached cotyledons, on the other hand, no conspicuous change in amylolytic activity was detected. When cotyledons were allowed to imbibe water through the surface not covered by the seed coat, amylase activity developed, even if they were detached from the axis. However, the developmental patterns of the activity were quite different from those with attached cotyledons; high amylase activity was detected in the cell layers beneath the epidermis of the surface not covered by the seed coat. These histochemical observations are related to previous results of biochemical experiments. Key words: amylase development, cotyledons, germination, histochemistry, mung bean, Vigna radiata.

Identification of the Cellular Location of Internalized Escherichia coli O157:H7 in Mung Bean, Vigna radiata, by Immunocytochemical Techniques

2011 ◽  
Vol 74 (8) ◽  
pp. 1224-1230 ◽  
Author(s):  
AMANDA J. DEERING ◽  
ROBERT E. PRUITT ◽  
LISA J. MAUER ◽  
BRADLEY L. REUHS
Keyword(s):  
Escherichia Coli ◽  
Plant Tissue ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Fluorescent Protein ◽  
Vascular Bundles ◽  
Escherichia Coli O157 ◽  
Cortical Cells ◽  
E Coli ◽  
Immunocytochemical Techniques

Escherichia coli O157:H7 has been associated with numerous outbreaks involving fresh produce. Previous studies have shown that bacteria can be internalized within plant tissue and that this can be a source of protection from antimicrobial chemicals and environmental conditions. However, the types of tissue and cellular locations the bacteria occupy in the plant following internalization have not been addressed. In this study, immunocytochemical techniques were used to localize internalized E. coli O157:H7 expressing green fluorescent protein in germinated mung bean (Vigna radiata) hypocotyl tissue following contamination of intact seeds. An average of 13 bacteria per mm3 were localized within the sampled tissue. The bacteria were found to be associated with every major tissue and corresponding cell type (cortex, phloem, xylem, epidermis, and pith). The cortical cells located on the outside of the vascular bundles contained the majority of the internalized bacteria (61%). In addition, the bacteria were localized primarily to the spaces between the cells (apoplast) and not within the cells. Growth experiments were also performed and demonstrated that mung bean plants could support the replication of bacteria to high levels (107 CFU per plant) following seed contamination and that these levels could be sustained over a 12-day period. Therefore, E. coli O157:H7 can be internalized in many different plant tissue types after a brief seed contamination event, and the bacteria are able to grow and persist within the plant.

Linking changes in chlorophyll a fluorescence with drought stress susceptibility in mung bean [ Vigna radiata (L.) Wilczek]

2021 ◽  
Author(s):  
Hussan Bano ◽  
Habib‐ur‐Rehman Athar ◽  
Zafar Ullah Zafar ◽  
Hazem M. Kalaji ◽  
Muhammad Ashraf
Keyword(s):  
Drought Stress ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Stress Susceptibility

Nanoplastic ingestion induces behavioral disorders in terrestrial snails: trophic transfer effects via vascular plants

2020 ◽  
Vol 7 (3) ◽  
pp. 975-983 ◽  
Author(s):  
Yooeun Chae ◽  
Youn-Joo An
Keyword(s):  
Vigna Radiata ◽  
Mung Bean ◽  
Vascular Plants ◽  
Trophic Transfer ◽  
Achatina Fulica ◽  
Transfer Effects ◽  
Terrestrial Environment ◽  
Plastic Debris ◽  
Terrestrial Snails ◽  
African Giant Snail

This study investigated the transfer of plastic debris in a terrestrial environment from the soil to a plant (the mung bean, Vigna radiata), and then to a consumer (the African giant snail, Achatina fulica).

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