STUDIES ON HUMAN ANTIBODIES

Human antibodies against dextran, teichoic acid, blood group A substance, levan, tetanus toxoid, and nuclei were isolated and analyzed for their content of Gm(a), Gm(b), and Inv(a) γ-globulin genetic factors. The majority of these antibodies contained all the genetic factors determined in the donor's whole γ-globulin, but in many antibodies at very different concentrations. In a few instances specific factors could not be detected despite their presence in the individual's whole γ-globulin. Different antibodies isolated from the serum of the same individual showed different relative concentrations of genetic factors. The distribution of genetic factors seen in certain isolated human antibodies appeared to approach the selective occurrence of these factors in myeloma proteins.

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STUDIES ON HUMAN ANTIBODIES

Journal of Experimental Medicine ◽

10.1084/jem.119.3.443 ◽

1964 ◽

Vol 119 (3) ◽

pp. 443-452 ◽

Cited By ~ 25

Author(s):

G. M. Edelman ◽

Elvin A. Kabat

Keyword(s):

Tetanus Toxoid ◽

Gel Electrophoresis ◽

Teichoic Acid ◽

Starch Gel Electrophoresis ◽

Human Antibodies ◽

Blood Group A ◽

Myeloma Proteins ◽

Group A ◽

Starch Gel ◽

Polypeptide Chains

Specific precipitates and purified human antibodies were reduced and alkylated and subjected to starch gel electrophoresis in 8 M urea to dissociate and separate the L and H polypeptide chains. Dissociated antibodies to dextran, levan, teichoic acid, blood group A substance, and tetanus toxoid showed sharp bands corresponding to L polypeptide chains. The patterns differed for antibodies of different specificities. Some differences were also seen among antibodies of the same specificity from different individuals. Purified antidextran antibodies showed particularly simple patterns resembling those of purified human γ myeloma proteins. In some cases only one L chain band was present.

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STUDIES ON HUMAN ANTIBODIES

Journal of Experimental Medicine ◽

10.1084/jem.129.5.871 ◽

1969 ◽

Vol 129 (5) ◽

pp. 871-896 ◽

Cited By ~ 27

Author(s):

Carlos Moreno ◽

Elvin A. Kabat

Keyword(s):

Density Gradient ◽

Blood Group ◽

Density Gradient Centrifugation ◽

Equilibrium Dialysis ◽

Gradient Centrifugation ◽

Light Chains ◽

Human Antibodies ◽

Blood Group A ◽

Group A

Human antibodies to blood group A substance were purified by absorption on columns of insoluble polyleucyl hog blood group A + H substance and eluted first with N-acetylgalactosamine and then with an A active reduced pentasaccharide ARL0.52. The γM and γG antibodies in these eluates were separated by density gradient centrifugation. The antibodies were studied for their relative capacities to be inhibited by various blood group A active oligosaccharides. Antibodies eluted by the N-acetylgalactosamine could be inhibited by N-acetylgalactosamine, as well as by lower concentrations of A active tri- and pentasaccharides, while those eluted by the pentasaccharide ARL0.52 could only be inhibited by the two oligosaccharides, but not by N-acetylgalactosamine, indicating that the N-acetylgalactosamine eluate had more antibodies with smaller size combining sites than the ARL0.52 eluate. Measurements by equilibrium dialysis gave values ranging from 2 x 103 to 1 x 105 M–1 and the values obtained with the ARL0.52 eluate were somewhat higher than those with the GalNAc eluate. Only one of three anti-A sera had γM anti-A in the ARL0.52 eluate, while all three had γM in the N-acetylgalactosamine eluate. Data on the precipitating, hemagglutinating, complement fixing, hemolytic properties of the eluted antibodies, and of their content of κ and λ light chains are given.

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Blood group and Human Leucocyte Antigen sub-type as determinants to keloid formation and Recurrence in Keloid Patients; A prospective longitudinal cohort study

10.21203/rs.3.rs-421812/v1 ◽

2021 ◽

Author(s):

Ferdinand Nangole ◽

Kelsey Ouyang ◽

George Agak ◽

Julius Ogeng'o ◽

Anzala Omu

Keyword(s):

Blood Group ◽

Genetic Factors ◽

Control Group ◽

Prospective Longitudinal Study ◽

Female Ratio ◽

Blood Group A ◽

Significant Difference ◽

Group A ◽

Surgical Conditions ◽

Prospective Longitudinal

Abstract The role of genetic factors in keloid is a firmed by the fact that keloids have been shown to occur among members of the same family.. We undertook this study to determine whether there is any association between patients’ bloodgroup and HLA sub-types to keloids and keloid recurrence. This was a prospective longitudinal study of patients with keloids and a control of patients managed for other surgical conditions with no keloids. Blood was taken from each patient and analysed for blood group and HLA sub-types using the sequence specific primer geno-typing. Data captured were summarized and analysed using students T-test to compare means. Probability values significance was at 0.05. A total of 90 patients with keloids and 59 in a control group were followed up in the study. The male to female ratio of the patients was 2:1. The most common blood group for both groups was blood group O at 51.3% and 49.2%, followed by blood group A and B respectively. Patients with keloids had a significantly higher positive alleles with DQA*01, DQB1*05, DQB1*06 and DRB1*15. There was an association between blood group A and DQB1*06 and recurrence. In conclusion, this study demonstrates that there is significant difference in HLA sub-types among patients who form keloids and the non-keloid forming patients among our study cohorts. Salient differences were also noted in patients with keloid recurrence based on their blood group, a pointer to the significance of genetic factors in keloid pathogenesis and severity.

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609: Reduced Expression of the Blood Group a Antigen is Caused by Allelic Loss and/or Hypermethylation of the ABO Gene on 9Q34.1 in Transitional Cell Carcinoma of the Bladder

The Journal of Urology ◽

10.1016/s0022-5347(18)34849-3 ◽

2005 ◽

Vol 173 (4S) ◽

pp. 166-166

Author(s):

Yoshitomo Chihara ◽

Kiyohide Fujimoto ◽

Yoshihiko Hirao ◽

Kokichi Sugano ◽

Yae Kanai ◽

...

Keyword(s):

Transitional Cell Carcinoma ◽

Cell Carcinoma ◽

Blood Group ◽

Transitional Cell ◽

Allelic Loss ◽

Blood Group A ◽

Group A ◽

Carcinoma Of The Bladder

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Faculty Opinions recommendation of Serum antibodies to blood group A predict survival on PROSTVAC-VF.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717987121.793472395 ◽

2013 ◽

Author(s):

Kevin Yarema

Keyword(s):

Blood Group ◽

Serum Antibodies ◽

Blood Group A ◽

Group A

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Isolation and characterization of novel glycolipids with blood group A-related structures: galactosyl-A and sialosylgalactosyl-A.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)47927-6 ◽

1987 ◽

Vol 262 (29) ◽

pp. 14228-14234

Author(s):

H Clausen ◽

S B Levery ◽

E D Nudelman ◽

M Stroud ◽

M E Salyan ◽

...

Keyword(s):

Blood Group ◽

Blood Group A ◽

Isolation And Characterization ◽

Group A

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Immunochemical studies on blood groups. Purification and characterization of radioactive 3H-reduced di- to hexasaccharides produced by alkaline beta-elimination-borohydride 3H reduction of Smith degraded blood group A active glycoproteins.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)39854-x ◽

1984 ◽

Vol 259 (11) ◽

pp. 7178-7186 ◽

Cited By ~ 6

Author(s):

A M Wu ◽

E A Kabat ◽

B Nilsson ◽

D A Zopf ◽

F G Gruezo ◽

...

Keyword(s):

Blood Group ◽

Blood Groups ◽

Purification And Characterization ◽

Blood Group A ◽

Group A ◽

Beta Elimination

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ABO groups can play a role in susceptibility and severity of COVID-19

Egyptian Journal of Bronchology ◽

10.1186/s43168-020-00051-w ◽

2021 ◽

Vol 15 (1) ◽

Author(s):

S. Samra ◽

M. Habeb ◽

R. Nafae

Keyword(s):

Blood Group ◽

Abo Blood Group ◽

Infection Group ◽

Mechanically Ventilated ◽

Blood Group A ◽

Group A ◽

Study Population ◽

Group B ◽

The Relationship ◽

Seriously Ill

Abstract Background A few people infected by the coronavirus become seriously ill, while others show little to no signs of the symptoms, or are asymptomatic. Recent researches are pointing to the fact that the ABO blood group might play an important role in a person’s susceptibility and severity of COVID-19 infection. Aim of the study: try to understand the relationship between ABO groups and COVID-19 (susceptibility and severity). Results A total of (507) patients were included in this study. The study population was divided based on the ABO blood group into types A+, A−, B+, AB, O+, and O−. Blood group A was associated with high susceptibility of infection: group A, 381 (75.1%); and less common in group O, 97 (19.2%), group B, 18 (3.5%), and group AB, 11 (2.2%). The severity of COVID-19 infection was common in non-blood group O where (20 (7.1%), 4 (26.7%), 2 (11%), and 1 (9%) in type A+, A−, B+, and AB, respectively), while in type O 3.1%. And mechanically ventilated patients were 22 (5.9%), 2 (13.4%), 2 (11.1%), and 1 (1%). Mortality was high in blood groups A and B, 16 (4.37%) and 1 (5.5%), respectively, while in blood group O, it was 1%. Conclusion The incidence, severity, and mortality of COVID-19 were common in non-blood group O. While blood group O was protected against COVID-19.

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