scholarly journals PEYER'S PATCHES: AN ENRICHED SOURCE OF PRECURSORS FOR IGA-PRODUCING IMMUNOCYTES IN THE RABBIT

1971 ◽  
Vol 134 (1) ◽  
pp. 188-200 ◽  
Author(s):  
Susan W. Craig ◽  
John J. Cebra
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Peripheral Blood ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Cell Transfer ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Donor Cells ◽  
Lymph Node Cells

The proliferative and differentiative potential of Peyer's patch, peripheral blood, and popliteal lymph node cells was assessed by allogeneic cell transfer followed by quantitation of donor immunocytes by immunofluorescence. It was found that Peyer's patches are a highly enriched source of cells which have the potential to proliferate and differentiate into IgA-producing immunocytes and that the Peyer's patch cells are far more efficient in seeding the gut of irradiated recipient rabbits with donor cells that give rise to immunoglobulin-producing cells than cells from peripheral blood or popliteal lymph nodes.

scholarly journals Peyer's Patch-Deficient Mice Demonstrate That Mycobacterium avium subsp. paratuberculosis Translocates across the Mucosal Barrier via both M Cells and Enterocytes but Has Inefficient Dissemination

2010 ◽  
Vol 78 (8) ◽  
pp. 3570-3577 ◽  
Author(s):  
Luiz E. Bermudez ◽  
Mary Petrofsky ◽  
Sandra Sommer ◽  
Raúl G. Barletta
Keyword(s):  
Mycobacterium Avium ◽  
Intestinal Mucosa ◽  
Peyer's Patches ◽  
Mucosal Barrier ◽  
Peyer’S Patches ◽  
M Cells ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Deficient Mice

ABSTRACT Mycobacterium avium subsp. paratuberculosis, the agent of Johne's disease, infects ruminant hosts by translocation through the intestinal mucosa. A number of studies have suggested that M. avium subsp. paratuberculosis interacts with M cells in the Peyer's patches of the small intestine. The invasion of the intestinal mucosa by M. avium subsp. paratuberculosis and Mycobacterium avium subsp. hominissuis, a pathogen known to interact with intestinal cells, was compared. M. avium subsp. paratuberculosis was capable of invading the mucosa, but it was significantly less efficient at dissemination than M. avium subsp. hominissuis. B-cell knockout (KO) mice, which lack Peyer's patches, were used to demonstrate that M. avium subsp. paratuberculosis enters the intestinal mucosa through enterocytes in the absence of M cells. In addition, the results indicated that M. avium subsp. paratuberculosis had equal abilities to cross the mucosa in both Peyer's patch and non-Peyer's patch segments of normal mice. M. avium subsp. paratuberculosis was also shown to interact with epithelial cells by an α5β1 integrin-independent pathway. Upon translocation, dendritic cells ingest M. avium subsp. paratuberculosis, but this process does not lead to efficient dissemination of the infection. In summary, M. avium subsp. paratuberculosis interacts with the intestinal mucosa by crossing both Peyer's patches and non-Peyer's patch areas but does not translocate or disseminate efficiently.

scholarly journals Secretory Immunoglobulin A Antibodies against the σ1 Outer Capsid Protein of Reovirus Type 1 Lang Prevent Infection of Mouse Peyer's Patches

2004 ◽  
Vol 78 (2) ◽  
pp. 947-957 ◽  
Author(s):  
Amy B. Hutchings ◽  
Anna Helander ◽  
Katherine J. Silvey ◽  
Kartik Chandran ◽  
William T. Lucas ◽  
...  
Keyword(s):  
Peyer's Patches ◽  
Peyer’S Patches ◽  
M Cells ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Adult Mice ◽  
Reovirus Type ◽  
Outer Capsid

ABSTRACT Reovirus type 1 Lang (T1L) adheres to M cells in the follicle-associated epithelium of mouse intestine and exploits the transport activity of M cells to enter and infect the Peyer's patch mucosa. Adult mice that have previously cleared a reovirus T1L infection have virus-specific immunoglobulin G (IgG) in serum and IgA in secretions and are protected against reinfection. Our aim in this study was to determine whether secretory IgA is sufficient for protection of Peyer's patches against oral reovirus challenge and, if so, against which reovirus antigen(s) the IgA may be directed. Monoclonal antibodies (MAbs) of the IgA isotype, directed against the σ1 protein of reovirus T1L, the viral adhesin, were produced and tested along with other, existing IgA and IgG MAbs against reovirus T1L outer capsid proteins. Anti-σ1 IgA and IgG MAbs neutralized reovirus T1L in L cell plaque reduction assays and inhibited T1L adherence to L cells and Caco-2BBe intestinal epithelial cells in vitro, but MAbs against other proteins did not. Passive oral administration of anti-σ1 IgA and IgG MAbs prevented Peyer's patch infection in adult mice, but other MAbs did not. When anti-σ1 IgA and IgG MAbs were produced in mice from hybridoma backpack tumors, however, the IgA prevented Peyer's patch infection, but the IgG did not. The results provide evidence that neutralizing IgA antibodies specific for the σ1 protein are protective in vitro and in vivo and that the presence of these antibodies in intestinal secretions is sufficient for protection against entry of reovirus T1L into Peyer's patches.

scholarly journals STUDIES ON THE TRANSFER OF LYMPH NODE CELLS

1954 ◽  
Vol 100 (3) ◽  
pp. 269-287 ◽  
Author(s):  
Susanna Harris ◽  
T. N. Harris
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Cell Suspension ◽  
Characteristic Curve ◽  
Transfer Effect ◽  
Cell Transfer ◽  
Lymph Node Cells ◽  
Hind Foot ◽  
Original Cell ◽  
Antigen Antibody

At various intervals, from 10 minutes to 21 days, after the injection of dysentery bacilli into the hind foot pads of rabbits the popliteal lymph nodes were excised. The cells of the lymph nodes were teased free, washed, and injected intravenously into normal rabbits. In each case aliquots of the same cell suspension were either incubated at 37°C. for 24 hours or heated at 52°C. for 20 minutes and then injected into other normal rabbits, as controls. In the case of lymph node cells obtained 4 or 3 days after the injection of antigen, antibody was found in the serum of recipients on the 1st day after the transfer of untreated cells. The titer increased until the 3rd day and then began to decline after the 5th or 7th day. In the sera of recipients of incubated cells antibody was not found, except on occasion after the 4th day and in low titer. This late appearance of antibody was attributed to the presence of small amounts of antigen in the original cell suspension. As the interval between injection of antigen and collection of cells was increased beyond 4 days the effectiveness of the transfer decreased progressively until at 14 days no transfer effect was obtained. When cells which were obtained 2 days after the injection of antigen were transferred, antibody appeared on the 2nd day after transfer and then followed the characteristic curve, whereas in the case of incubated cells antibody did not appear until the 3rd day after transfer. After the transfer of untreated 1 day cells antibody did not appear in the recipient until the 3rd day, and then followed the type of curve seen with 2, 3, and 4 day cells. Following transfer of incubated 1 day cells antibody also appeared on the 3rd day. To establish the possibility of eliciting the cell transfer effect as early as 1 day after the injection of dysentery bacilli, recipient rabbits were x-irradiated 24 hours prior to the injection of cells. It was found that in the sera of such recipients of untreated cells antibody appeared on the 3rd day following transfer, while irradiated recipients of incubated cells did not develop any measurable amounts of agglutinin for the first 10 days. It was concluded that a total of 3 days was required between the injection of antigen into the donor and the appearance of measurable antibody in the serum of the recipient, regardless of the fraction of that time spent by the cells in each of the animals involved, donor or recipient. Following the transfer of untreated cells removed from lymph node as early as 10 minutes after the injection of antigen distal to them, antibody could be found in the sera of x-irradiated recipients 4 days later, whereas antibody did not appear following the transfer of heated cells to such recipients.

scholarly journals Modulation of lymphocyte subsets in Peyer's patches of mice treated with monoclonal antibody against helper T-cells.

1988 ◽  
Vol 36 (4) ◽  
pp. 417-423 ◽  
Author(s):  
T H Ermak ◽  
H J Steger ◽  
R L Owen ◽  
M F Heyworth
Keyword(s):  
Monoclonal Antibody ◽  
T Cells ◽  
Lymphocyte Subsets ◽  
Germinal Centers ◽  
Peyer's Patches ◽  
Helper T Cells ◽  
Size Difference ◽  
Peyer’S Patches ◽  
Peyer’S Patch ◽  
Peyer's Patch

Treatment of mice with anti-L3T4, a monoclonal antibody directed against helper T-cells, impairs clearance of intestinal Giardia muris infection. The present study examined the effect of anti-L3T4 treatment on mouse Peyer's patch cytoarchitecture and on the distribution of T-cell subsets within microenvironments of the follicle. Female BALB/c mice, aged 8 weeks, were given 4-7 weekly injections of either anti-L3T4 (1 mg/wk) or PBS (control group), and Peyer's patches were examined by immunohistochemistry or flow cytometry. In anti-L3T4-treated mice, Peyer's patch follicles (B-cell areas) were about two thirds the size of follicles in controls, and virtually all the size difference occurred in germinal centers. Peyer's patches were depleted of L3T4+ cells, yet the proportion of Thy-1.2+ (all T) cells was not decreased correspondingly, and the distribution of Thy-1.2+ cells in the patches was similar to that in control mice. In anti-L3T4-treated mice, Thy-1.2+ cells consisted of (a) Ly-2+ (cytotoxic/suppressor T) cells, and (b) a population of Thy-1.2+ cells that were neither L3T4+ nor Ly-2+. After treatment, Ly-2+ cells accounted for most of the T-cells in interfollicular areas and were also scattered in follicles, in germinal centers, and below the dome epithelium--in areas where L3T4+ cells predominated in control mice. Thy-1.2+ cells that were L3T4- and Ly-2- were mainly localized below the dome epithelium. These shifts indicate complex interrelationships among different lymphocyte subsets in Peyer's patches.

scholarly journals THE DISTRIBUTION OF LARGE DIVIDING LYMPH NODE CELLS IN SYNGENEIC RECIPIENT RATS AFTER INTRAVENOUS INJECTION

1969 ◽  
Vol 130 (6) ◽  
pp. 1427-1451 ◽  
Author(s):  
Claude Griscelli ◽  
Pierre Vassalli ◽  
Robert T. McCluskey
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Thoracic Duct ◽  
Lymphoid Tissue ◽  
Thoracic Duct Lymph ◽  
Donor Cells ◽  
Duct Lymph ◽  
Lymph Node Cells ◽  
Syngeneic Recipient

The distribution of large dividing lymph node or thoracic duct lymph cells, labeled in vitro with 3H-thymidine, was studied in syngeneic recipient rats after intravenous injection. In most experiments the donor rats had been immunized with Bacillus pertussis 4 days earlier, but in some instances cells from nonimmunized donors were used. In smears, the labeled donor cells had the appearance of large lymphocytes or large pyroninophilic cells. By electronmicroscopy, the majority of labeled donor cells were seen to have only scanty endoplasmic reticulum. It was found that the labeled cells rapidly "homed" to lymphoid tissue and recirculated in the recipient, in a fashion resembling that of small lymphocytes. However, the distribution of labeled cells was found to depend upon the source of the donor cells. Cells from mesenteric lymph nodes or thoracic duct lymph showed a marked preferential accumulation in lymphoid tissue within or adjacent to the intestine, whereas cells from peripheral nodes accumulated preferentially in peripheral lymph nodes. Cells from any of these sources showed an equal tendency to accumulate in the white pulp of the spleen. Suspensions of small lymphocytes, labeled in vitro with 3H-uridine, did not display a similar tendency to localize preferentially in lymphoid tissue in certain regions. It was also found that large dividing lymph node cells from donors immunized with an antigen (2,4-dinitrophenyl-bovine gamma globulin (DNP-BGG) or B. pertussis) showed a greater tendency to accumulate in a recipient lymph node containing that antigen than in the contralateral node. It was not determined whether the selective accumulation of large dividing lymphoid cells from different sources in lymphoid tissue of different regions in recipients was due to an antigen recognition mechansim or was the result of two different populations of cells with different "homing" mechanisms.

scholarly journals Imunotoksisitas pewarna makanan terhadap histopatologi Peyer’s patch goblet mencit (The immunotoxicity of food additive on histopatology of mice Peyer’s patch goblet)

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
Muhammad HF Amin ◽  
Ida BR Pidada ◽  
Cicik SB Utami
Keyword(s):  
Food Additives ◽  
Relative Weight ◽  
Treated Group ◽  
Peyer's Patches ◽  
Spleen Weight ◽  
Peyer’S Patches ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Food Colorant ◽  
Metanil Yellow

Abstrak Zat aditif dalam makanan digunakan untuk memperbaiki karakter pangan agar mutunya meningkat. Pewarna makanan merupakan suatu substansi yang ditambahkan ke dalam makanan untuk merubah warna, aroma dan estetika. Penelitian ini bertujuan untuk mengetahui histopatologi Peyer’s patch mencit akibat paparan pewarna sintetik dan alami. Ada tiga pewarna yang digunakan dalam penelitian ini, yaitu curcumin, tartrazine, dan metanil yellow. Penelitian ini bersifat eksperimental laboratoris menggunakan mencit sebagai hewan coba yang diperlakukan selama empat minggu dengan pemberian ketiga jenis pewarna dengan dosis 90 mg/kg BB. Di akhir perlakuan, intestinum bagian ileum diambil dan dibuat sediaan histologi untuk pengamatan histologi Peyer’s patch. Hasil pengukuran dimensi Peyer’s patch, menunjukkan bahwa pemberian pewarna makanan mampu menginduksi penurunan dimensi Peyer’s patch dan berkorelasi positif dengan berat relatif limpa. Dimensi Peyer’s patch yang paling kecil adalah kelompok yang diberi perlakuan tartrazine, linear dengan penurunan berat relatif limpa yang signifikan. Hal ini memperkuat indikasi efek imunosupresor akibat pemberian tartrazine. Pemberian curcumin dan metanil yellow terlihat tidak mempengaruhi komponen sistem imun secara signifikan berdasarkan penurunan berat relatif limpa dan dimensi Peyer’s patch, meskipun terdapat tren penurunan. Kata kunci : berat relatif limpa, pewarna makanan, Peyer’s patch Abstract Food additives used to improve quality of food in order to increase the character. Food colorant is a substance added to food to change the color, flavor and aesthetics. This study aims to determine the histopathology Peyer's patches of mice caused by exposure of synthetic and natural dyes. There are three dyes used in this study, e.g curcumin, tartrazine, and metanil yellow. This research is an experimental laboratory using mice as experimental animals were treated for four weeks with three types of dye administration at a dose of 90 mg / kg b.wt. At the end of treatment, ileum were taken and histological preparations were made for histological observation of Peyer's patches. The results of dimensions measurements of Peyer's patches, suggesting that administration of food colorings able to induce dimension reduction Peyer's patches and positively correlated with relative weight of the spleen. The smallest dimension of Peyer's patches are tartrazine treated group, a linear decrease in relative spleen weight significantly. This strengthens the indication of the imunosupresor effects caused by administration tartrazine. Curcumin and Metanil yellow not affect significantly the immune system components based on relative spleen weight and dimensions of the Peyer's patches, despite the downward trend. Keywords: food colorant, Peyer's patches, relative spleen weight

Kinetics of particle uptake in the domes of Peyer’s patches

1998 ◽  
Vol 275 (1) ◽  
pp. G130-G137 ◽  
Author(s):  
Rita Beier ◽  
Andreas Gebert
Keyword(s):  
Basal Lamina ◽  
Peyer's Patches ◽  
Yeast Cells ◽  
Lymphoid Tissues ◽  
Peyer’S Patches ◽  
M Cells ◽  
Peyer’S Patch ◽  
Peyer's Patch ◽  
Particle Uptake ◽  
Kinetics Of

Uptake of particulate antigenic matter, including microorganisms and vaccine-bearing microspheres, by the intestinal mucosa takes place in the domes of the gut-associated lymphoid tissues and is achieved by membranous (M) cells, which continuously transport particles from the lumen to the underlying tissue where some particle components initiate immune reactions. Using yeast as tracer, we investigated the kinetics of particle uptake in the Peyer’s patches of pigs. A suspension of baker’s yeast ( Saccharomyces cerevisiae) was injected into the gut lumen of anesthetized minipigs; the position of yeast cells in the tissue was determined after 1, 2.5, 4, and 24 h using fluorescence light- and thin-section electron microscopy. After 1 h, 18.5% of all M cells had taken up or were in close contact with yeast cells. The intercellular space of the epithelium contained a maximum of 60.8% of all yeast cells found in the tissue after 2.5 h, but only 1.3% had been phagocytosed by macrophages. After 4 h most yeast cells (77.8%) were found beneath the basal lamina, and most of these (89%) were found in macrophages. No yeast cells were detected in the Peyer’s patch domes 24 h after application. The data show that transcytosis of yeast particles (3.4 ± 0.8 μm in diameter) by M cells takes <1 h. Without significant phagocytosis by intraepithelial macrophages, the particles migrate down to and across the basal lamina within 2.5–4 h, where they quickly get phagocytosed and transported out of the Peyer’s patch domes.

Sign in / Sign up

Export Citation Format

Share Document