scholarly journals A HIGH SPEED VACUUM CENTRIFUGE SUITABLE FOR THE STUDY OF FILTERABLE VIRUSES

1936 ◽  
Vol 64 (4) ◽  
pp. 503-528 ◽  
Author(s):  
Johannes H. Bauer ◽  
Edward G. Pickels

1. A high speed centrifuge is described in which the speed is limited only by the strength of the material of which the rotor is made. It carries sixteen tubes, each of which conveniently accommodates 7 cc. of fluid. 2. The centrifuge operates in a very high vacuum and therefore requires only a small amount of driving energy. The arrangement has been found to eliminate the possibility of producing injurious frictional heat. 3. The rotating parts are supported by anair-bearing and are driven by compressed air. 4. The centrifuge has been successfully operated at a speed of 30,000 revolutions per minute, representing a maximum centrifugal force in the fluid of 95,000 times gravity. 5 Celluloid tubes used for centrifugation of fluid at high speeds are described. 6. Experiments are described in which good sedimentation of the yellow fever virus was obtained.

2021 ◽  
Vol 6 ◽  
pp. 664-677
Author(s):  
Tara Sadeghieh ◽  
Jan M. Sargeant ◽  
Amy L. Greer ◽  
Olaf Berke ◽  
Guillaume Dueymes ◽  
...  

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1475
Author(s):  
Moussa Moïse Diagne ◽  
Marie Henriette Dior Ndione ◽  
Alioune Gaye ◽  
Mamadou Aliou Barry ◽  
Diawo Diallo ◽  
...  

Yellow fever virus remains a major threat in low resource countries in South America and Africa despite the existence of an effective vaccine. In Senegal and particularly in the eastern part of the country, periodic sylvatic circulation has been demonstrated with varying degrees of impact on populations in perpetual renewal. We report an outbreak that occurred from October 2020 to February 2021 in eastern Senegal, notified and managed through the synergistic effort yellow fever national surveillance implemented by the Senegalese Ministry of Health in collaboration with the World Health Organization, the countrywide 4S network set up by the Ministry of Health, the Institut Pasteur de Dakar, and the surveillance of arboviruses and hemorrhagic fever viruses in human and vector populations implemented since mid 2020 in eastern Senegal. Virological analyses highlighted the implication of sylvatic mosquito species in virus transmission. Genomic analysis showed a close relationship between the circulating strain in eastern Senegal, 2020, and another one from the West African lineage previously detected and sequenced two years ago from an unvaccinated Dutch traveler who visited the Gambia and Senegal before developing signs after returning to Europe. Moreover, genome analysis identified a 6-nucleotide deletion in the variable domain of the 3′UTR with potential impact on the biology of the viral strain that merits further investigations. Integrated surveillance of yellow fever virus but also of other arboviruses of public health interest is crucial in an ecosystem such as eastern Senegal.


1929 ◽  
Vol 92 (7) ◽  
pp. 550 ◽  
Author(s):  
HENRIQUE DE BEAUREPAIRE ARAGÃO

1995 ◽  
Vol 35 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Claudia N. Duarte dos Santos ◽  
Paulo R. Post ◽  
Ricardo Carvalho ◽  
Idevaldo I. Ferreira ◽  
Charles M. Rice ◽  
...  

2002 ◽  
Vol 2 (1) ◽  
pp. 47-50 ◽  
Author(s):  
B. Mondet ◽  
P.F.C. Vasconcelos ◽  
A.P.A. Travassos da Rosa ◽  
E.S. Travassos da Rosa ◽  
S.G. Rodrigues ◽  
...  

2015 ◽  
Vol 9 (4) ◽  
pp. e0003693 ◽  
Author(s):  
Milton Maciel ◽  
Fábia da Silva Pereira Cruz ◽  
Marli Tenório Cordeiro ◽  
Márcia Archer da Motta ◽  
Klécia Marília Soares de Melo Cassemiro ◽  
...  

Virology ◽  
2012 ◽  
Vol 422 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Laura K. McMullan ◽  
Mike Frace ◽  
Scott A. Sammons ◽  
Trevor Shoemaker ◽  
Stephen Balinandi ◽  
...  

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