A long-term load test performed for 470 days on two two-span prestressed concrete (PC) continuous box girders is reported in this paper. Load types were selected as the test variates, and structural responses such as support reactions, deflections, and concrete strains were monitored. Simultaneously, affiliated experiments such as material strength, creep, and shrinkage tests were conducted to investigate the time-dependent performances of the materials. Data obtained from these tests showed that deflections, strains, and support reactions develop rapidly in the beginning and stabilize afterward; the reactions of mid- and end-supports decline and rise over time, respectively. Time-dependent patterns of deflections and support reactions were analyzed on the basis of an effective modulus method, and a practical calculation method for long-term deflections considering reaction redistributions was proposed. The effects of the service environment on the performance of PC girders were evaluated through an incremental analysis method.