Effects of Ionizing Radiation and Long-Term Storage on Hydrated vs. Dried Cell Samples of Extremophilic Microorganisms

A main factor hampering life in space is represented by high atomic number nuclei and energy (HZE) ions that constitute about 1% of the galactic cosmic rays. In the frame of the “STARLIFE” project, we accessed the Heavy Ion Medical Accelerator (HIMAC) facility of the National Institute of Radiological Sciences (NIRS) in Chiba, Japan. By means of this facility, the extremophilic species Haloterrigena hispanica and Parageobacillus thermantarcticus were irradiated with high LET ions (i.e., Fe, Ar, and He ions) at doses corresponding to long permanence in the space environment. The survivability of HZE-treated cells depended upon either the storage time and the hydration state during irradiation; indeed, dry samples were shown to be more resistant than hydrated ones. With particular regard to spores of the species P. thermantarcticus, they were the most resistant to irradiation in a water medium: an analysis of the changes in their biochemical fingerprinting during irradiation showed that, below the survivability threshold, the spores undergo to a germination-like process, while for higher doses, inactivation takes place as a consequence of the concomitant release of the core’s content and a loss of integrity of the main cellular components. Overall, the results reported here suggest that the selected extremophilic microorganisms could serve as biological model for space simulation and/or real space condition exposure, since they showed good resistance to ionizing radiation exposure and were able to resume cellular growth after long-term storage.

Measurement of Energy Spectrum and Elemental Composition of PeV Cosmic Rays: Open Problems and Prospects

Cosmic rays represent one of the most important energy transformation processes of the universe. They bring information about the surrounding universe, our galaxy, and very probably also the extragalactic space, at least at the highest observed energies. More than one century after their discovery, we have no definitive models yet about the origin, acceleration and propagation processes of the radiation. The main reason is that there are still significant discrepancies among the results obtained by different experiments located at ground level, probably due to unknown systematic uncertainties affecting the measurements. In this document, we will focus on the detection of galactic cosmic rays from ground with air shower arrays up to 1018 eV. The aim of this paper is to discuss the conflicting results in the 1015 eV energy range and the perspectives to clarify the origin of the so-called `knee’ in the all-particle energy spectrum, crucial to give a solid basis for models up to the end of the cosmic ray spectrum. We will provide elements useful to understand the basic techniques used in reconstructing primary particle characteristics (energy, mass, and arrival direction) from the ground, and to show why indirect measurements are difficult and results are still conflicting.

Observational Constraints on the Maximum Energies of Accelerated Particles in Supernova Remnants: Low Maximum Energies and a Large Variety

Supernova remnants (SNRs) are thought to be the most promising sources of Galactic cosmic rays. One of the principal questions is whether they are accelerating particles up to the maximum energy of Galactic cosmic rays (∼PeV). In this work, a systematic study of gamma-ray-emitting SNRs is conducted as an advanced study of Suzuki et al. Our purpose is to newly measure the evolution of maximum particle energies with increased statistics and better age estimates. We model their gamma-ray spectra to constrain the particle-acceleration parameters. Two candidates of the maximum energy of freshly accelerated particles, the gamma-ray cutoff and break energies, are found to be well below PeV. We also test a spectral model that includes both the freshly accelerated and escaping particles to estimate the maximum energies more reliably, but no tighter constraints are obtained with current statistics. The average time dependences of the cutoff energy (∝t −0.81±0.24) and break energy (∝t −0.77±0.23) cannot be explained with the simplest acceleration condition (Bohm limit) and require shock–ISM (interstellar medium) interaction. The average maximum energy during lifetime is found to be ≲20 TeV ( t M / 1 kyr ) − 0.8 with t M being the age at the maximum, which reaches PeV if t M ≲ 10 yr. The maximum energies during lifetime are suggested to have a variety of 1.1–1.8 dex from object to object. Although we cannot isolate the cause of this variety, this work provides an important clue to understanding the microphysics of particle acceleration in SNRs.

The Two-step Forbush Decrease: A Tale of Two Substructures Modulating Galactic Cosmic Rays within Coronal Mass Ejections

Interplanetary coronal mass ejections (ICMEs) are known to modify the structure of the solar wind as well as interact with the space environment of planetary systems. Their large magnetic structures have been shown to interact with galactic cosmic rays (GCRs), leading to the Forbush decrease (FD) phenomenon. We revisit in the present article the 17 yr of Advanced Composition Explorer spacecraft ICME detection along with two neutron monitors (McMurdo and Oulu) with a superposed epoch analysis to further analyze the role of the magnetic ejecta in driving FDs. We investigate in the following the role of the sheath and the magnetic ejecta in driving FDs, and we further show that for ICMEs without a sheath, a magnetic ejecta only is able to drive significant FDs of comparable intensities. Furthermore, a comparison of samples with and without a sheath with similar speed profiles enable us to show that the magnetic field intensity, rather than its fluctuations, is the main driver for the FD. Finally, the recovery phase of the FD for isolated magnetic ejecta shows an anisotropy in the level of the GCRs. We relate this finding at 1 au to the gradient of the GCR flux found at different heliospheric distances from several interplanetary missions.

A Numerical Study of the Solar Modulation of Galactic Protons and Helium from 2006 to 2017

With continuous measurements from space-borne cosmic-ray detectors such as AMS-02 and PAMELA, precise spectra of galactic cosmic rays over the 11 yr solar cycle have become available. For this study, we utilize proton and helium spectra below 10 GV from these missions from 2006 to 2017 to construct a cosmic-ray transport model for a quantitative study of the processes of solar modulation. This numerical model is based on Parker’s transport equation, which includes four major transport processes. The Markov Chain Monte Carlo method is utilized to search the relevant parameter space related to the drift and the diffusion coefficients by reproducing and fitting the mentioned observed spectra. The resulting best-fit normalized χ 2 is mainly less than 1. It is found that (1) when reproducing these observations the parameters required for the drift and diffusion coefficients exhibit a clear time dependence, with the magnitude of the diffusion coefficients anticorrelated with solar activity; (2) the rigidity dependence of the resulting mean free paths varies with time, and their rigidity dependence at lower rigidity can even have a larger slope than at higher rigidity; (3) using a single set of modulation parameters for each pair of observed proton and helium spectra, most spectra are reproduced within observational uncertainty; and (4) the simulated proton-to-helium flux ratio agrees with the observed values in terms of its long-term time dependence, although some discrepancy exists, and the difference is mostly coming from the underestimation of proton flux.

Amifostine (WR-2721) Mitigates Cognitive Injury Induced by Heavy Ion Radiation in Male Mice and Alters Behavior and Brain Connectivity

The deep space environment contains many risks to astronauts during space missions, such as galactic cosmic rays (GCRs) comprised of naturally occurring heavy ions. Heavy ion radiation is increasingly being used in cancer therapy, including novel regimens involving carbon therapy. Previous investigations involving simulated space radiation have indicated a host of detrimental cognitive and behavioral effects. Therefore, there is an increasing need to counteract these deleterious effects of heavy ion radiation. Here, we assessed the ability of amifostine to mitigate cognitive injury induced by simulated GCRs in C57Bl/6J male and female mice. Six-month-old mice received an intraperitoneal injection of saline, 107 mg/kg, or 214 mg/kg of amifostine 1 h prior to exposure to a simplified five-ion radiation (protons, 28Si, 4He, 16O, and 56Fe) at 500 mGy or sham radiation. Mice were behaviorally tested 2–3 months later. Male mice that received saline and radiation exposure failed to show novel object recognition, which was reversed by both doses of amifostine. Conversely, female mice that received saline and radiation exposure displayed intact object recognition, but those that received amifostine prior to radiation did not. Amifostine and radiation also had distinct effects on males and females in the open field, with amifostine affecting distance moved over time in both sexes, and radiation affecting time spent in the center in females only. Whole-brain analysis of cFos immunoreactivity in male mice indicated that amifostine and radiation altered regional connectivity in areas involved in novel object recognition. These data support that amifostine has potential as a countermeasure against cognitive injury following proton and heavy ion irradiation in males.

Direct Numerical Simulations of Cosmic-ray Acceleration at Dense Circumstellar Medium: Magnetic-field Amplification and Maximum Energy

Galactic cosmic rays are believed to be accelerated at supernova remnants. However, whether supernova remnants can be PeV is still very unclear. In this work we argue that PeV cosmic rays can be accelerated during the early phase of a supernova blast-wave expansion in dense red supergiant winds. We solve in spherical geometry a system combining a diffusive–convection equation that treats cosmic-ray dynamics coupled to magnetohydrodynamics to follow gas dynamics. A fast shock expanding in a dense ionized wind is able to trigger fast, non-resonant streaming instability over day timescales and energizes cosmic rays even under the effect of p–p losses. We find that such environments produce PeV blast waves, although the maximum energy depends on various parameters such as the injection rate and mass-loss rate of the winds. Multi-PeV energies can be reached if the progenitor mass-loss rates are of the order of 10−3 M ⊙ yr−1. It has been recently proposed that, prior to the explosion, hydrogen-rich massive stars can produce enhanced mass-loss rates. These enhanced rates would then favor the production of a PeV phase in early times after shock breakout.